Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   MON40_RS13115 Genome accession   NZ_CP094241
Coordinates   2741748..2742794 (+) Length   348 a.a.
NCBI ID   WP_003779408.1    Uniprot ID   A0AA36UIS5
Organism   Neisseria macacae ATCC 33926     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2736748..2747794
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MON40_RS13090 (MON40_13090) dksA 2738414..2738830 (-) 417 WP_003767066.1 RNA polymerase-binding protein DksA -
  MON40_RS13095 (MON40_13095) - 2739024..2739578 (-) 555 WP_003779401.1 YggT family protein -
  MON40_RS13100 (MON40_13100) proC 2739581..2740378 (-) 798 WP_039863169.1 pyrroline-5-carboxylate reductase -
  MON40_RS13105 (MON40_13105) - 2740469..2740864 (-) 396 WP_003779405.1 hypothetical protein -
  MON40_RS13110 (MON40_13110) - 2740861..2741568 (-) 708 WP_003779407.1 YggS family pyridoxal phosphate-dependent enzyme -
  MON40_RS13115 (MON40_13115) pilT 2741748..2742794 (+) 1047 WP_003779408.1 type IV pilus twitching motility protein PilT Machinery gene
  MON40_RS13120 (MON40_13120) - 2742963..2744195 (+) 1233 WP_003779409.1 PilT/PilU family type 4a pilus ATPase -
  MON40_RS13125 (MON40_13125) - 2744290..2744640 (+) 351 WP_242925942.1 helix-turn-helix domain-containing protein -
  MON40_RS13130 (MON40_13130) - 2744544..2745440 (+) 897 WP_242925943.1 IS3 family transposase -
  MON40_RS13135 (MON40_13135) - 2745841..2746377 (+) 537 WP_003767081.1 porin family protein -

Sequence

Protein


Download         Length: 348 a.a.        Molecular weight: 38142.59 Da        Isoelectric Point: 7.1310

>NTDB_id=668324 MON40_RS13115 WP_003779408.1 2741748..2742794(+) (pilT) [Neisseria macacae ATCC 33926]
MQITDLLAFGVKNKASDLHLSAGMSPMIRVHGDIRRINLPEMSAEEVGNMVTSVMNDHQRKLFQQDLEVDFSFELPNVAR
FRVNAFTSNRGPAAVFRTIPSDVLTLEDLRAPRIFQKIADNPRGLVLVTGPTGSGKSTTLAAMINYINETQPAHILTIED
PIEFVHQSKKALVNQRELHQHTHSFANALRSALREDPDVILVGEMRDPETIGLALTAAETGHLVFGTLHTTGAAKTVDRI
VDVFPAGEKEMVRSMLSESLRAVISQTLLKTRDGNGRVAAHEILISTPAVRNLIRENKIAQINSALQTGQAHGMQTLDQA
LQTLVRQGTISPDVARSKAQNSDSMTIV

Nucleotide


Download         Length: 1047 bp        

>NTDB_id=668324 MON40_RS13115 WP_003779408.1 2741748..2742794(+) (pilT) [Neisseria macacae ATCC 33926]
ATGCAGATTACCGACTTACTCGCTTTCGGCGTCAAAAACAAAGCTTCCGACCTTCACTTAAGCGCCGGAATGTCCCCCAT
GATCCGTGTTCACGGCGACATCCGCCGCATCAACCTGCCCGAAATGTCCGCCGAAGAAGTCGGCAACATGGTTACTTCGG
TCATGAACGACCATCAGCGCAAACTGTTCCAACAAGATTTGGAAGTGGACTTCTCCTTCGAGCTGCCCAACGTCGCCCGT
TTCCGTGTCAACGCATTTACCTCCAACCGCGGTCCTGCCGCCGTCTTCCGTACCATTCCGAGCGACGTATTGACGCTGGA
AGACCTGCGCGCCCCACGTATTTTCCAAAAAATCGCCGATAACCCGCGCGGCTTGGTACTGGTAACCGGTCCGACCGGTT
CCGGTAAATCCACCACGCTGGCGGCGATGATCAACTACATCAACGAAACCCAACCCGCACACATCCTCACCATCGAAGAC
CCGATCGAGTTCGTCCACCAAAGTAAAAAAGCCCTCGTCAACCAGCGCGAGTTGCACCAACACACCCACAGCTTTGCCAA
CGCCCTGCGTTCCGCATTGCGTGAAGACCCGGACGTGATTCTCGTGGGCGAGATGCGCGACCCCGAAACCATCGGTCTTG
CGCTGACCGCCGCCGAAACCGGCCACTTGGTATTCGGTACGCTGCACACCACCGGCGCAGCCAAAACCGTAGACCGTATC
GTCGACGTATTCCCTGCCGGCGAAAAAGAAATGGTGCGTTCGATGCTGTCCGAGTCCCTGCGCGCGGTCATTTCGCAAAC
CCTGCTGAAAACCCGCGACGGCAACGGCCGTGTTGCCGCACACGAAATTCTCATCTCCACGCCCGCAGTGCGCAACCTGA
TCCGCGAAAACAAAATCGCCCAAATCAACTCCGCCCTGCAAACCGGTCAAGCACACGGCATGCAAACCCTCGACCAAGCG
CTGCAAACCCTCGTCCGCCAAGGCACCATCAGCCCCGACGTAGCCCGCAGTAAAGCGCAAAACAGCGACAGCATGACCAT
CGTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Neisseria meningitidis 8013

88.184

99.713

0.879
  pilT Neisseria gonorrhoeae MS11

87.608

99.713

0.874
  pilT Acinetobacter baumannii D1279779

71.014

99.138

0.704
  pilT Acinetobacter baumannii strain A118

71.014

99.138

0.704
  pilT Acinetobacter nosocomialis M2

71.014

99.138

0.704
  pilT Pseudomonas stutzeri DSM 10701

69.855

99.138

0.693
  pilT Pseudomonas aeruginosa PAK

70

97.701

0.684
  pilT Acinetobacter baylyi ADP1

68.406

99.138

0.678
  pilT Legionella pneumophila strain Lp02

66.862

97.989

0.655
  pilT Legionella pneumophila strain ERS1305867

66.862

97.989

0.655
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

65

97.701

0.635
  pilT Vibrio cholerae strain A1552

65

97.701

0.635
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.622

97.414

0.503
  pilU Pseudomonas stutzeri DSM 10701

41.954

100

0.42
  pilU Vibrio cholerae strain A1552

39.368

100

0.394
  pilU Acinetobacter baylyi ADP1

40.361

95.402

0.385

Multiple sequence alignment