Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   MNR06_RS12185 Genome accession   NZ_CP093442
Coordinates   2601309..2602403 (-) Length   364 a.a.
NCBI ID   WP_243536418.1    Uniprot ID   -
Organism   Bdellovibrio reynosensis strain LBG001     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2596309..2607403
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MNR06_RS12160 (MNR06_12160) - 2596629..2597861 (-) 1233 WP_243536408.1 S8 family peptidase -
  MNR06_RS12165 (MNR06_12165) - 2598070..2598885 (-) 816 WP_243536410.1 purine-nucleoside phosphorylase -
  MNR06_RS12170 (MNR06_12170) - 2598896..2600215 (-) 1320 WP_243536412.1 thymidine phosphorylase -
  MNR06_RS12175 (MNR06_12175) deoC 2600215..2600877 (-) 663 WP_243536414.1 deoxyribose-phosphate aldolase -
  MNR06_RS12180 (MNR06_12180) - 2600937..2601308 (+) 372 WP_243536416.1 hypothetical protein -
  MNR06_RS12185 (MNR06_12185) pilT 2601309..2602403 (-) 1095 WP_243536418.1 type IV pilus twitching motility protein PilT Machinery gene
  MNR06_RS12190 (MNR06_12190) - 2602532..2604049 (+) 1518 WP_243536420.1 Rne/Rng family ribonuclease -
  MNR06_RS12195 (MNR06_12195) - 2604085..2604381 (-) 297 WP_243536422.1 helix-turn-helix domain-containing protein -
  MNR06_RS12200 (MNR06_12200) - 2604854..2606314 (+) 1461 WP_243536424.1 hypothetical protein -
  MNR06_RS12205 (MNR06_12205) rplU 2606522..2607079 (+) 558 WP_243536425.1 50S ribosomal protein L21 -
  MNR06_RS12210 (MNR06_12210) rpmA 2607110..2607370 (+) 261 WP_243536427.1 50S ribosomal protein L27 -

Sequence

Protein


Download         Length: 364 a.a.        Molecular weight: 39692.81 Da        Isoelectric Point: 7.9844

>NTDB_id=664679 MNR06_RS12185 WP_243536418.1 2601309..2602403(-) (pilT) [Bdellovibrio reynosensis strain LBG001]
MATIDELFKLMVEQGASDLHITSGAPPYLRLHGNMVPLNYRELTNQDVQGLLFEILSEKQKKAFVEKWELDFAYTLSGIG
RFRCNIFMQRKGLGAVMRIIPEKIKTAQELGLPPAVMDMIDCDRGLILVTGPTGSGKSTTLAAMIHQINMTREAHIITVE
DPIEFVHPNLKALVNQREVGSHTKSFANALKAALREDPDILLVGELRDLETISLALTAAETGHIVFGTLHTNSAAKTVDR
IIDVFPAGQQQQIRTMLAESLRGVVAQTLFSRADGQGRVAAYEIMRNTKAISNLIREGKVHQLASAMQTGSSQGMVLFEK
YIEDLVRKGKVSAADAKTFLGQAGGGDTAIQPNAATQATRTKVG

Nucleotide


Download         Length: 1095 bp        

>NTDB_id=664679 MNR06_RS12185 WP_243536418.1 2601309..2602403(-) (pilT) [Bdellovibrio reynosensis strain LBG001]
ATGGCAACAATTGATGAACTGTTTAAATTGATGGTGGAACAAGGAGCTTCCGACTTGCATATCACAAGCGGCGCGCCTCC
TTACCTTCGACTGCATGGAAACATGGTGCCTTTGAATTACCGTGAATTGACGAACCAAGACGTACAAGGCTTGCTCTTCG
AAATTCTTTCTGAAAAACAAAAGAAAGCCTTCGTTGAAAAATGGGAACTTGATTTCGCTTACACTCTTTCTGGAATTGGT
CGCTTCCGTTGCAATATCTTTATGCAGCGTAAAGGACTTGGCGCAGTAATGCGTATTATCCCTGAGAAAATTAAAACAGC
GCAAGAGTTAGGTTTACCTCCTGCGGTGATGGATATGATTGATTGTGATCGTGGTTTGATTCTTGTTACGGGTCCCACAG
GTTCTGGTAAATCTACGACCTTGGCTGCCATGATTCATCAAATCAATATGACTCGCGAAGCCCACATCATCACGGTTGAA
GACCCGATCGAGTTTGTTCATCCGAATTTGAAAGCGCTTGTGAATCAACGCGAAGTGGGAAGTCATACAAAAAGTTTCGC
TAACGCTTTAAAAGCAGCCTTACGTGAAGATCCAGATATTTTGCTTGTGGGTGAGTTGCGTGACCTTGAAACTATTTCTT
TAGCATTAACGGCTGCAGAAACGGGTCACATTGTGTTTGGTACTCTTCATACGAACAGTGCTGCAAAAACAGTCGATCGT
ATCATTGACGTTTTCCCCGCGGGACAACAACAACAAATCCGTACGATGCTGGCAGAAAGCTTGCGTGGCGTAGTGGCGCA
AACTCTTTTCTCGCGCGCCGATGGACAAGGTCGTGTCGCGGCTTACGAAATCATGCGTAATACAAAAGCCATCTCGAATC
TTATTCGCGAAGGAAAAGTGCATCAATTGGCTTCGGCAATGCAAACAGGTTCAAGCCAAGGCATGGTGCTATTTGAAAAA
TACATTGAGGATCTGGTTCGCAAAGGGAAGGTTTCTGCTGCTGATGCAAAAACCTTCTTGGGCCAGGCTGGTGGTGGCGA
CACAGCGATTCAACCGAATGCTGCGACCCAAGCGACCCGCACCAAAGTGGGCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(13-258)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.689

99.725

0.505
  pilT Pseudomonas aeruginosa PAK

55.09

91.758

0.505
  pilT Acinetobacter baumannii D1279779

54.627

92.033

0.503
  pilT Acinetobacter baumannii strain A118

54.627

92.033

0.503
  pilT Acinetobacter nosocomialis M2

54.627

92.033

0.503
  pilT Pseudomonas stutzeri DSM 10701

54.491

91.758

0.5
  pilT Acinetobacter baylyi ADP1

54.192

91.758

0.497
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

54.03

92.033

0.497
  pilT Vibrio cholerae strain A1552

54.03

92.033

0.497
  pilT Legionella pneumophila strain Lp02

54.711

90.385

0.495
  pilT Legionella pneumophila strain ERS1305867

54.711

90.385

0.495
  pilT Neisseria meningitidis 8013

50.599

91.758

0.464
  pilT Neisseria gonorrhoeae MS11

50.599

91.758

0.464
  pilU Pseudomonas stutzeri DSM 10701

42.73

92.582

0.396
  pilU Vibrio cholerae strain A1552

42.025

89.56

0.376
  pilU Acinetobacter baylyi ADP1

41.018

91.758

0.376