Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   MOQ58_RS27740 Genome accession   NZ_CP093428
Coordinates   6075279..6076313 (-) Length   344 a.a.
NCBI ID   WP_008036678.1    Uniprot ID   -
Organism   Pseudomonas migulae strain 8R6     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6070279..6081313
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MOQ58_RS27710 (MOQ58_27670) ruvX 6070412..6070849 (+) 438 WP_007945492.1 Holliday junction resolvase RuvX -
  MOQ58_RS27715 (MOQ58_27675) pyrR 6070932..6071435 (+) 504 WP_008026190.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  MOQ58_RS27720 (MOQ58_27680) - 6071463..6072467 (+) 1005 WP_008026189.1 aspartate carbamoyltransferase catalytic subunit -
  MOQ58_RS27725 (MOQ58_27685) - 6072464..6073735 (+) 1272 WP_008026186.1 dihydroorotase -
  MOQ58_RS27730 (MOQ58_27690) - 6073830..6074261 (-) 432 WP_008026184.1 TM2 domain-containing protein -
  MOQ58_RS27735 (MOQ58_27695) - 6074512..6075132 (+) 621 WP_008026181.1 C40 family peptidase -
  MOQ58_RS27740 (MOQ58_27700) pilT 6075279..6076313 (-) 1035 WP_008036678.1 type IV pilus twitching motility protein PilT Machinery gene
  MOQ58_RS27745 (MOQ58_27705) - 6076510..6077196 (+) 687 WP_008026178.1 YggS family pyridoxal phosphate-dependent enzyme -
  MOQ58_RS27750 (MOQ58_27710) proC 6077233..6078051 (+) 819 WP_207284875.1 pyrroline-5-carboxylate reductase -
  MOQ58_RS27755 (MOQ58_27715) - 6078076..6078663 (+) 588 WP_192227547.1 YggT family protein -
  MOQ58_RS27760 (MOQ58_27720) - 6078874..6080013 (+) 1140 WP_280162423.1 homoserine O-acetyltransferase -
  MOQ58_RS27765 (MOQ58_27725) metW 6080021..6080641 (+) 621 WP_008026169.1 methionine biosynthesis protein MetW -
  MOQ58_RS27770 (MOQ58_27730) - 6080668..6081102 (+) 435 WP_192227546.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38094.68 Da        Isoelectric Point: 6.8287

>NTDB_id=664669 MOQ58_RS27740 WP_008036678.1 6075279..6076313(-) (pilT) [Pseudomonas migulae strain 8R6]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPALDHKEVQELVYDIMNDTQRVDFEKHLETDFSFEVPGVAR
FRVNAFNQSRGAGAVFRTIPSKVLSMEDLGMGEVFRKITDSPRGLVLVTGPTGSGKSTTLAAMIDYLNNHRHHHILTIED
PIEFVHESRKCLINQREVHRDTRSFATALRSALREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRI
VDVFPGDEKSMVRSMLSESLLAVVSQTLVKKIGGGRIAAHEIMLGTSAIRNLIREDKVAQMYSSIQTGGNLGMQTLDMCL
KELVTKGLISREHARDKARSPDNF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=664669 MOQ58_RS27740 WP_008036678.1 6075279..6076313(-) (pilT) [Pseudomonas migulae strain 8R6]
ATGGATATCACTGAGCTGCTGGCGTTCAGCGCCAAACAAGGAGCGTCCGACTTGCACCTGTCGGCAGGCCTGCCACCGAT
GATTCGCGTCGACGGCGATGTGCGGCGCATCAATCTGCCAGCCCTGGATCACAAGGAAGTTCAGGAGCTGGTCTACGACA
TCATGAACGACACCCAGCGTGTGGATTTCGAGAAGCACCTGGAAACCGACTTTTCCTTTGAGGTGCCCGGCGTTGCGCGT
TTCCGGGTCAATGCGTTCAACCAGAGCCGTGGCGCCGGCGCGGTGTTCCGAACGATTCCGTCGAAGGTCCTGAGCATGGA
AGACCTCGGGATGGGGGAAGTGTTTCGCAAGATTACCGACTCCCCCCGTGGCCTGGTTTTGGTCACCGGCCCGACCGGTT
CCGGCAAGTCCACCACCCTGGCGGCGATGATCGACTACCTGAACAACCATCGTCATCACCACATCCTGACCATTGAAGAC
CCGATCGAATTCGTCCACGAATCACGCAAATGCCTGATCAATCAGCGCGAAGTCCATCGCGATACCCGCAGCTTCGCCAC
CGCCTTGCGCTCGGCGTTGCGGGAAGACCCGGATGTGATTCTGGTGGGCGAGATGCGTGATCTGGAGACTATTCGCCTGG
CGCTGACGGCTGCCGAGACTGGCCATCTGGTGTTCGGCACGTTGCACACCACGTCGGCGGCGAAAACCATCGACCGGATC
GTGGATGTGTTTCCGGGGGATGAGAAGTCGATGGTGCGTTCGATGTTGTCCGAGTCGCTGCTGGCGGTGGTATCGCAGAC
GCTGGTGAAGAAAATCGGCGGCGGGCGGATCGCGGCGCACGAAATCATGCTGGGAACGTCGGCGATCCGTAACCTGATCC
GCGAGGACAAGGTGGCGCAGATGTACTCGTCGATTCAGACCGGCGGGAATCTGGGGATGCAGACGCTGGATATGTGCTTG
AAGGAGTTGGTGACCAAAGGCTTGATCAGCCGCGAGCATGCGCGGGATAAGGCGCGGTCGCCGGATAATTTTTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

86.628

100

0.866
  pilT Pseudomonas aeruginosa PAK

85.756

100

0.858
  pilT Acinetobacter nosocomialis M2

79.07

100

0.791
  pilT Acinetobacter baumannii D1279779

79.07

100

0.791
  pilT Acinetobacter baumannii strain A118

79.07

100

0.791
  pilT Acinetobacter baylyi ADP1

78.198

100

0.782
  pilT Legionella pneumophila strain Lp02

72.093

100

0.721
  pilT Legionella pneumophila strain ERS1305867

72.093

100

0.721
  pilT Neisseria meningitidis 8013

67.246

100

0.674
  pilT Neisseria gonorrhoeae MS11

66.957

100

0.672
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.249

97.965

0.669
  pilT Vibrio cholerae strain A1552

68.249

97.965

0.669
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.014

100

0.512
  pilU Pseudomonas stutzeri DSM 10701

41.449

100

0.416
  pilU Acinetobacter baylyi ADP1

40.462

100

0.407
  pilU Vibrio cholerae strain A1552

40.29

100

0.404