Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   PSCI_RS10145 Genome accession   NZ_AP014637
Coordinates   2200210..2201244 (+) Length   344 a.a.
NCBI ID   WP_045485932.1    Uniprot ID   A0A077LFA7
Organism   Pseudomonas sp. StFLB209     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2195210..2206244
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PSCI_RS10115 (PSCI_1999) - 2195577..2196020 (-) 444 WP_045485914.1 DUF4426 domain-containing protein -
  PSCI_RS10120 (PSCI_2000) metW 2196040..2196660 (-) 621 WP_045485917.1 methionine biosynthesis protein MetW -
  PSCI_RS10125 (PSCI_2001) - 2196668..2197807 (-) 1140 WP_045485920.1 homoserine O-acetyltransferase -
  PSCI_RS10130 (PSCI_2002) - 2197991..2198581 (-) 591 WP_045485923.1 YggT family protein -
  PSCI_RS10135 (PSCI_2003) proC 2198610..2199431 (-) 822 WP_045485926.1 pyrroline-5-carboxylate reductase -
  PSCI_RS10140 (PSCI_2004) - 2199456..2200154 (-) 699 WP_045485929.1 YggS family pyridoxal phosphate-dependent enzyme -
  PSCI_RS10145 (PSCI_2005) pilT 2200210..2201244 (+) 1035 WP_045485932.1 type IV pilus twitching motility protein PilT Machinery gene
  PSCI_RS10150 (PSCI_2006) - 2201358..2202038 (-) 681 WP_045485935.1 C40 family peptidase -
  PSCI_RS10155 (PSCI_2007) - 2202287..2202694 (+) 408 WP_045485937.1 TM2 domain-containing protein -
  PSCI_RS10160 (PSCI_2008) - 2202919..2204190 (-) 1272 WP_045485939.1 dihydroorotase -
  PSCI_RS10165 (PSCI_2009) - 2204187..2205191 (-) 1005 WP_045485942.1 aspartate carbamoyltransferase catalytic subunit -
  PSCI_RS10170 (PSCI_2010) pyrR 2205204..2205722 (-) 519 WP_045485945.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37954.71 Da        Isoelectric Point: 7.0292

>NTDB_id=66441 PSCI_RS10145 WP_045485932.1 2200210..2201244(+) (pilT) [Pseudomonas sp. StFLB209]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPALDAKQVKALIYDIMNDKQRQDYEERLETDFSFEVPGVAR
FRVNAFNQNRGSGAVFRTIPSKILTLDDLGMGENFRRITDVPRGLILVTGPTGSGKSTTLAAMIDYLNSNKHHHILTIED
PIEFVHESKKCLVNQREVHRDTLGFSEALRSALREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRI
VDVFPAAEKSMIRSMLSESLHAVISQALLKKVGGGRVAAHEIMMGTPAIRNLIREDKVAQMYSAIQTGGALGMQTLDMCL
AELVKRGLITRDNAREKAKVPDNF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=66441 PSCI_RS10145 WP_045485932.1 2200210..2201244(+) (pilT) [Pseudomonas sp. StFLB209]
ATGGATATTACCGAACTGCTGGCCTTCAGTGCCAAACAGGGCGCCTCCGATCTGCACCTTTCCGCAGGCTTGCCACCGAT
GATTCGTGTCGACGGTGACGTGCGGCGGATCAACCTGCCGGCGCTGGATGCCAAGCAGGTCAAGGCGCTGATTTACGACA
TCATGAACGACAAGCAGCGCCAGGATTATGAAGAACGCCTGGAAACCGACTTTTCTTTCGAAGTCCCCGGCGTGGCGCGC
TTTCGGGTCAACGCTTTCAACCAGAACCGTGGCAGTGGCGCGGTGTTTCGCACCATTCCGTCGAAGATCCTCACCCTTGA
TGACCTCGGCATGGGCGAGAACTTTCGGCGCATCACTGACGTGCCACGCGGGCTGATTCTGGTCACCGGGCCTACCGGGT
CAGGCAAGTCGACCACCCTGGCGGCAATGATCGATTACCTCAACAGCAACAAGCATCACCATATCCTGACCATCGAAGAC
CCGATCGAATTCGTCCACGAGTCCAAGAAGTGCCTGGTCAACCAGCGTGAAGTGCACCGCGACACGCTGGGCTTTTCCGA
AGCCTTGCGCTCGGCGCTGCGTGAAGACCCGGACGTGATTCTGGTCGGCGAGATGCGCGACCTTGAAACCATCCGCCTGG
CGCTGACGGCGGCTGAAACCGGCCACCTGGTGTTCGGCACCCTGCACACCACCTCGGCGGCCAAGACCATCGACCGGATT
GTCGATGTGTTTCCGGCTGCGGAAAAGTCGATGATCCGCTCGATGCTCTCAGAGTCGCTGCACGCGGTGATTTCGCAGGC
GTTGTTGAAGAAGGTGGGCGGTGGCCGGGTGGCGGCCCATGAAATCATGATGGGCACGCCGGCAATCCGTAACCTGATTC
GTGAAGACAAGGTCGCGCAGATGTATTCCGCGATCCAGACCGGTGGCGCGCTGGGCATGCAGACCCTGGATATGTGCCTG
GCCGAGCTGGTCAAGCGTGGCCTGATTACCCGCGACAACGCGCGGGAGAAGGCCAAGGTGCCGGATAACTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A077LFA7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

88.953

100

0.89
  pilT Pseudomonas aeruginosa PAK

88.081

100

0.881
  pilT Acinetobacter nosocomialis M2

79.942

100

0.799
  pilT Acinetobacter baumannii D1279779

79.942

100

0.799
  pilT Acinetobacter baumannii strain A118

79.942

100

0.799
  pilT Acinetobacter baylyi ADP1

78.488

100

0.785
  pilT Legionella pneumophila strain Lp02

73.077

98.256

0.718
  pilT Legionella pneumophila strain ERS1305867

73.077

98.256

0.718
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.843

97.965

0.674
  pilT Vibrio cholerae strain A1552

68.843

97.965

0.674
  pilT Neisseria meningitidis 8013

67.647

98.837

0.669
  pilT Neisseria gonorrhoeae MS11

67.353

98.837

0.666
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.623

100

0.538
  pilU Acinetobacter baylyi ADP1

41.04

100

0.413
  pilU Vibrio cholerae strain A1552

39.71

100

0.398
  pilU Pseudomonas stutzeri DSM 10701

40.299

97.384

0.392

Multiple sequence alignment