Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilE   Type   Machinery gene
Locus tag   MNR01_RS14770 Genome accession   NZ_CP093341
Coordinates   3288993..3289421 (-) Length   142 a.a.
NCBI ID   WP_241918516.1    Uniprot ID   -
Organism   Lysobacter sp. S4-A87     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 3279747..3297388 3288993..3289421 within 0


Gene organization within MGE regions


Location: 3279747..3297388
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MNR01_RS14730 (MNR01_14730) - 3279747..3280604 (+) 858 WP_241918508.1 glycosyltransferase family 2 protein -
  MNR01_RS14735 (MNR01_14735) - 3280604..3281332 (+) 729 WP_241918509.1 class I SAM-dependent methyltransferase -
  MNR01_RS14740 (MNR01_14740) - 3281272..3282156 (+) 885 WP_241918510.1 class I SAM-dependent methyltransferase -
  MNR01_RS14745 (MNR01_14745) - 3282171..3283124 (+) 954 WP_241918511.1 hypothetical protein -
  MNR01_RS14750 (MNR01_14750) - 3283152..3284297 (-) 1146 WP_241918512.1 glycosyltransferase family 4 protein -
  MNR01_RS14755 (MNR01_14755) pilE 3284365..3284784 (-) 420 WP_241918513.1 pilin Machinery gene
  MNR01_RS14760 (MNR01_14760) - 3284971..3286491 (-) 1521 WP_241918514.1 hypothetical protein -
  MNR01_RS14765 (MNR01_14765) - 3286973..3288919 (-) 1947 WP_241918515.1 hypothetical protein -
  MNR01_RS14770 (MNR01_14770) pilE 3288993..3289421 (-) 429 WP_241918516.1 pilin Machinery gene
  MNR01_RS14775 (MNR01_14775) pilR 3289836..3291284 (-) 1449 WP_241918517.1 sigma-54 dependent transcriptional regulator Regulator
  MNR01_RS14780 (MNR01_14780) - 3291416..3293038 (-) 1623 WP_241918518.1 ATP-binding protein -
  MNR01_RS14785 (MNR01_14785) sucC 3293271..3294437 (+) 1167 WP_241918519.1 ADP-forming succinate--CoA ligase subunit beta -
  MNR01_RS14790 (MNR01_14790) sucD 3294450..3295325 (+) 876 WP_241918520.1 succinate--CoA ligase subunit alpha -
  MNR01_RS14795 (MNR01_14795) - 3295451..3297388 (+) 1938 WP_256451847.1 acyltransferase family protein -

Sequence

Protein


Download         Length: 142 a.a.        Molecular weight: 14466.61 Da        Isoelectric Point: 9.0171

>NTDB_id=663833 MNR01_RS14770 WP_241918516.1 3288993..3289421(-) (pilE) [Lysobacter sp. S4-A87]
MKNNQQGFTLIELMIVVAIIAILAAIALPAYQDYVARSQVSEAMSLASGAKTAVAEQYANVGSFASITNATAGLAVAGSI
KGDYVSQVTVAGGVVTATMGGKASKKLVPGTLVMSPSDQGGSISWTCTNSTVNNKYLPSSCR

Nucleotide


Download         Length: 429 bp        

>NTDB_id=663833 MNR01_RS14770 WP_241918516.1 3288993..3289421(-) (pilE) [Lysobacter sp. S4-A87]
ATGAAGAACAATCAGCAAGGCTTCACCCTGATCGAACTGATGATCGTCGTGGCGATCATCGCCATCCTGGCCGCGATCGC
GCTGCCGGCTTACCAGGACTACGTCGCTCGTAGCCAGGTGTCGGAAGCGATGAGCCTGGCTTCGGGCGCCAAGACCGCCG
TGGCCGAGCAGTACGCCAACGTCGGCTCCTTCGCCTCGATCACCAATGCCACCGCCGGCCTGGCCGTTGCTGGCTCGATC
AAGGGCGACTACGTCTCCCAGGTCACCGTCGCCGGTGGCGTCGTCACCGCCACGATGGGCGGCAAGGCCAGCAAGAAGCT
CGTCCCGGGCACGCTCGTCATGTCGCCGAGCGACCAGGGCGGCAGCATCTCCTGGACCTGCACCAACAGCACCGTCAACA
ACAAGTACCTGCCGAGCAGCTGCCGCTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(36-142)

Prokaryotic N-terminal methylation motif (N_methyl)

(2-28)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Neisseria gonorrhoeae strain FA1090

47.799

100

0.535
  pilE Neisseria gonorrhoeae MS11

45.625

100

0.514
  pilA Ralstonia pseudosolanacearum GMI1000

40.964

100

0.479
  pilA2 Legionella pneumophila str. Paris

46.099

99.296

0.458
  pilA2 Legionella pneumophila strain ERS1305867

45.39

99.296

0.451
  pilA/pilA1 Eikenella corrodens VA1

40

100

0.437
  comP Acinetobacter baylyi ADP1

40.94

100

0.43
  pilA Pseudomonas aeruginosa PAK

39.474

100

0.423
  pilA Acinetobacter baumannii strain A118

38.514

100

0.401
  pilA/pilAI Pseudomonas stutzeri DSM 10701

41.304

97.183

0.401
  pilA Vibrio cholerae C6706

37.241

100

0.38
  pilA Vibrio cholerae strain A1552

37.241

100

0.38
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

37.241

100

0.38
  pilA/pilAII Pseudomonas stutzeri DSM 10701

36.62

100

0.366