Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   MNR01_RS06985 Genome accession   NZ_CP093341
Coordinates   1533483..1534520 (-) Length   345 a.a.
NCBI ID   WP_241920201.1    Uniprot ID   -
Organism   Lysobacter sp. S4-A87     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1528483..1539520
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MNR01_RS06960 (MNR01_06960) ruvX 1528627..1529124 (-) 498 WP_241920196.1 Holliday junction resolvase RuvX -
  MNR01_RS06965 (MNR01_06965) - 1529124..1529690 (-) 567 WP_241920197.1 YqgE/AlgH family protein -
  MNR01_RS06970 (MNR01_06970) - 1529781..1531508 (+) 1728 WP_241920198.1 Zn-dependent hydrolase -
  MNR01_RS06975 (MNR01_06975) - 1531512..1532075 (+) 564 WP_241920199.1 DNA-3-methyladenine glycosylase I -
  MNR01_RS06980 (MNR01_06980) pilU 1532216..1533361 (-) 1146 WP_241920200.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  MNR01_RS06985 (MNR01_06985) pilT 1533483..1534520 (-) 1038 WP_241920201.1 type IV pilus twitching motility protein PilT Machinery gene
  MNR01_RS06990 (MNR01_06990) - 1534705..1535361 (+) 657 WP_241920551.1 YggS family pyridoxal phosphate-dependent enzyme -
  MNR01_RS06995 (MNR01_06995) proC 1535416..1536261 (+) 846 WP_241920202.1 pyrroline-5-carboxylate reductase -
  MNR01_RS07000 (MNR01_07000) - 1536294..1536767 (+) 474 WP_241920203.1 DUF4426 domain-containing protein -
  MNR01_RS07005 (MNR01_07005) - 1536770..1537564 (-) 795 WP_241920552.1 cobalamin-binding protein -
  MNR01_RS07010 (MNR01_07010) pdxH 1537582..1538184 (-) 603 WP_241920204.1 pyridoxamine 5'-phosphate oxidase -
  MNR01_RS07015 (MNR01_07015) - 1538202..1539071 (+) 870 WP_241920205.1 kinase -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38544.31 Da        Isoelectric Point: 6.5777

>NTDB_id=663823 MNR01_RS06985 WP_241920201.1 1533483..1534520(-) (pilT) [Lysobacter sp. S4-A87]
MDIAELLAFSVKNKASDLHLSAGLPPMIRVDGDVRRINIPALDHKQVHALVYDIMSDKQRRDFEEFLEVDFSFEIPGLAR
FRVNAFNQNRGAGAVFRTIPSEVLALDDLGCPPIFRELIDQPQGLILVTGPTGSGKSTTLAAMIDHINKNEYGHILSVED
PIEFVHTSQKCLINQREVHRDTHGFNEALRSALREDPDYILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTVDRI
IDVFPAGEKPMVRSMLSESLRAVISQALLKKVGGGRTAAWEIMVGIPAIRNLIREDKVAQMYSSIQTGQQYGMMTLDQHL
QDLVKRGLILRPQAREYAKDKRLFE

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=663823 MNR01_RS06985 WP_241920201.1 1533483..1534520(-) (pilT) [Lysobacter sp. S4-A87]
ATGGATATCGCCGAACTTCTGGCGTTTTCGGTAAAGAACAAAGCATCCGACCTGCACCTGTCTGCGGGCTTGCCTCCGAT
GATCCGGGTCGACGGCGACGTCCGCCGCATCAACATCCCGGCCCTGGACCACAAGCAGGTGCACGCGCTGGTCTACGACA
TCATGTCGGACAAGCAGCGTCGCGACTTCGAAGAATTCCTCGAGGTCGACTTCTCCTTCGAGATTCCCGGCCTGGCGCGT
TTCCGCGTCAACGCCTTCAACCAGAACCGCGGCGCCGGTGCGGTGTTCCGTACCATTCCCTCGGAAGTGCTGGCCCTGGA
CGACCTGGGTTGCCCGCCGATCTTCCGCGAGCTGATCGACCAGCCGCAGGGCCTGATCCTGGTGACCGGCCCGACCGGCT
CGGGCAAGTCGACCACGCTGGCGGCGATGATCGACCACATCAACAAGAACGAATACGGCCACATCCTCTCGGTCGAGGAT
CCGATCGAGTTCGTCCACACCTCGCAGAAGTGCCTGATCAACCAGCGCGAAGTGCACCGCGACACGCACGGATTCAACGA
AGCGCTGCGCTCGGCCCTGCGCGAAGACCCCGACTACATCCTGGTCGGCGAGTTGCGCGACCTGGAGACCATCCGCCTGG
CGCTGACCGCCGCGGAAACCGGCCACCTGGTGTTCGGCACCCTGCACACCTCCTCGGCGGCCAAGACGGTCGACCGAATC
ATCGACGTGTTCCCCGCCGGCGAAAAGCCGATGGTGCGCTCGATGCTGTCGGAGTCGCTGCGCGCGGTGATCTCGCAGGC
GCTGCTGAAGAAGGTCGGCGGCGGTCGTACCGCGGCCTGGGAAATCATGGTCGGCATCCCGGCGATCCGTAACCTGATCC
GCGAGGACAAGGTCGCGCAGATGTACTCGTCGATCCAGACCGGCCAGCAGTACGGAATGATGACGCTCGACCAGCATCTG
CAAGACCTGGTCAAGCGCGGCCTGATCCTGCGTCCGCAGGCGCGCGAGTACGCCAAGGACAAGCGTCTTTTCGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

78.779

99.71

0.786
  pilT Acinetobacter baumannii D1279779

78.779

99.71

0.786
  pilT Acinetobacter baumannii strain A118

78.779

99.71

0.786
  pilT Pseudomonas stutzeri DSM 10701

78.198

99.71

0.78
  pilT Acinetobacter baylyi ADP1

77.907

99.71

0.777
  pilT Pseudomonas aeruginosa PAK

77.876

98.261

0.765
  pilT Legionella pneumophila strain Lp02

73.837

99.71

0.736
  pilT Legionella pneumophila strain ERS1305867

73.837

99.71

0.736
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.301

95.362

0.661
  pilT Vibrio cholerae strain A1552

69.301

95.362

0.661
  pilT Neisseria meningitidis 8013

65.396

98.841

0.646
  pilT Neisseria gonorrhoeae MS11

64.912

99.13

0.643
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.275

100

0.493
  pilU Acinetobacter baylyi ADP1

40.588

98.551

0.4
  pilU Pseudomonas stutzeri DSM 10701

40.413

98.261

0.397
  pilU Vibrio cholerae strain A1552

40.308

94.203

0.38