Detailed information NTG0663814

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: MNO14_RS13275
• Genome accession: NZ_CP093340
• Coordinates: 2933252..2934364 (+)
• Length: 370 a.a.
• NCBI ID: WP_241944191.1
• Uniprot ID: -
• Organism: Luteimonas sp. S4-F44
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
Genomic island	2916449..2939792	2933252..2934364	within	0

Gene organization within MGE regions

Location: 2916449..2939792

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
MNO14_RS13215 (MNO14_13215)	-	2916488..2917477 (+)	990	WP_241944180.1	glycosyltransferase	-
MNO14_RS13220 (MNO14_13220)	-	2917582..2920224 (-)	2643	WP_241944181.1	hypothetical protein	-
MNO14_RS13225 (MNO14_13225)	-	2920357..2922621 (-)	2265	WP_241944182.1	glycosyltransferase family 2 protein	-
MNO14_RS13230 (MNO14_13230)	-	2922625..2924826 (-)	2202	WP_241944183.1	glycosyltransferase family 2 protein	-
MNO14_RS13235 (MNO14_13235)	-	2924830..2926467 (-)	1638	WP_241944184.1	class I SAM-dependent methyltransferase	-
MNO14_RS13240 (MNO14_13240)	-	2926464..2927813 (-)	1350	WP_241944185.1	ABC transporter ATP-binding protein	-
MNO14_RS13245 (MNO14_13245)	-	2927803..2928555 (-)	753	WP_241944186.1	ABC transporter permease	-
MNO14_RS13250 (MNO14_13250)	-	2928638..2929819 (-)	1182	WP_241944187.1	cystathionine gamma-synthase	-
MNO14_RS13255 (MNO14_13255)	-	2929993..2931369 (-)	1377	WP_241944188.1	pyridoxal-phosphate dependent enzyme	-
MNO14_RS13260 (MNO14_13260)	-	2931498..2931719 (-)	222	WP_047137910.1	YdcH family protein	-
MNO14_RS13265 (MNO14_13265)	-	2932199..2932774 (-)	576	WP_241944189.1	hypothetical protein	-
MNO14_RS13270 (MNO14_13270)	-	2932777..2933157 (-)	381	WP_241944190.1	DUF4398 domain-containing protein	-
MNO14_RS13275 (MNO14_13275)	pilU	2933252..2934364 (+)	1113	WP_241944191.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
MNO14_RS13280 (MNO14_13280)	maiA	2934375..2935034 (-)	660	WP_241944192.1	maleylacetoacetate isomerase	-
MNO14_RS13285 (MNO14_13285)	-	2935031..2936035 (-)	1005	WP_241944193.1	fumarylacetoacetate hydrolase family protein	-
MNO14_RS13290 (MNO14_13290)	-	2936094..2936828 (-)	735	WP_241944194.1	2OG-Fe dioxygenase family protein	-
MNO14_RS13300 (MNO14_13300)	rpsI	2937100..2937492 (-)	393	WP_183427676.1	30S ribosomal protein S9	-
MNO14_RS13305 (MNO14_13305)	rplM	2937496..2937924 (-)	429	WP_183427677.1	50S ribosomal protein L13	-
MNO14_RS13310 (MNO14_13310)	-	2938073..2938681 (-)	609	WP_241944195.1	methyltransferase domain-containing protein	-
MNO14_RS13315 (MNO14_13315)	-	2938761..2939792 (-)	1032	WP_241944196.1	hypothetical protein	-

Sequence

Protein

• Length: 370 a.a.
• Molecular weight: 41239.30 Da
• Isoelectric Point: 6.4434

>NTDB_id=663814 MNO14_RS13275 WP_241944191.1 2933252..2934364(+) (pilU) [Luteimonas sp. S4-F44]
MDIGPFLKLMTEKSASDMFLSTGAPIHIKIEGRLQPLEGTHLAPGVVRQIAYSMMDEQQIRRFERDLEMNMAYSLPDVGR
FRVNVFRQRGEAGMVVRAISSKIPDIAQLQLPQVLKDVITAPRGLVLIVGSTGSGKSTTLASMIDHRNTCSAGHILTIED
PIEYLHRHKRSLVNQREVGIDTQTFHNALKNAMREAPDVILIGEILDATTMEAAIAFAETGHLCLATLHSNNADQTIERI
LNFFPEAAHKNVLMNLSLNLRAVISQRLVEGVDGRRLPATEVLLNTPTIRDLLRRGHVHEVKQAMEESLEEGMETFDQCL
YRLYKSGRIERETALRAADSRDGLDLKFRLSEGAADAHDPYADVFAPQSL

Nucleotide

• Length: 1113 bp

>NTDB_id=663814 MNO14_RS13275 WP_241944191.1 2933252..2934364(+) (pilU) [Luteimonas sp. S4-F44]
ATGGACATCGGCCCGTTTCTGAAGCTGATGACGGAAAAAAGCGCGTCGGACATGTTCCTGAGCACCGGCGCGCCGATTCA
CATCAAGATCGAGGGCCGGCTGCAGCCGCTCGAAGGCACGCATCTGGCACCCGGGGTCGTCCGCCAGATCGCCTACTCGA
TGATGGACGAGCAGCAAATCCGGCGTTTCGAGCGCGATCTTGAGATGAACATGGCCTACTCGCTGCCCGACGTCGGCCGC
TTCCGCGTCAACGTCTTTCGCCAGCGCGGCGAGGCTGGCATGGTCGTGCGCGCGATCAGCAGCAAGATTCCCGACATCGC
GCAGTTGCAGTTGCCGCAGGTGCTCAAGGACGTGATCACCGCCCCACGCGGGCTGGTGCTGATCGTAGGCTCCACCGGCT
CGGGCAAGTCGACGACGCTGGCCTCGATGATCGACCACCGCAACACCTGCTCGGCCGGCCACATTCTCACGATCGAGGAC
CCGATCGAGTACCTGCACCGTCACAAGCGCTCGCTGGTCAACCAGCGCGAGGTCGGGATCGACACCCAGACCTTCCACAA
TGCGCTGAAGAATGCGATGCGCGAAGCGCCCGACGTGATCCTGATCGGCGAGATCCTCGACGCGACCACGATGGAGGCGG
CGATCGCGTTCGCCGAGACCGGGCACCTGTGTCTGGCCACCCTGCACTCCAACAACGCCGACCAGACGATCGAGCGCATC
CTCAATTTCTTCCCCGAGGCTGCGCACAAGAACGTGCTGATGAACCTGTCGCTGAACCTGCGCGCGGTGATCTCCCAACG
CCTGGTCGAGGGCGTGGACGGCCGCCGGCTGCCGGCGACCGAAGTGCTGCTCAACACGCCGACGATCCGCGACCTGCTGC
GCCGAGGCCATGTCCACGAGGTCAAGCAGGCGATGGAGGAATCGCTCGAGGAAGGCATGGAGACCTTCGACCAGTGCCTC
TATCGCCTCTACAAAAGCGGACGCATCGAGCGGGAGACCGCCCTGCGCGCGGCCGACTCGCGCGACGGCCTGGACCTCAA
GTTCCGCCTCTCCGAAGGCGCGGCCGACGCCCATGACCCGTACGCGGACGTGTTCGCCCCGCAATCGCTGTAG

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (12-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	51.253	97.027	0.497
pilU	Acinetobacter baylyi ADP1	48.315	96.216	0.465
pilU	Vibrio cholerae strain A1552	48.305	95.676	0.462
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	45.93	92.973	0.427
pilT	Pseudomonas aeruginosa PAK	43.491	91.351	0.397
pilT	Acinetobacter baumannii strain A118	42.899	91.351	0.392
pilT	Acinetobacter nosocomialis M2	42.899	91.351	0.392
pilT	Acinetobacter baumannii D1279779	42.899	91.351	0.392
pilT	Pseudomonas stutzeri DSM 10701	42.604	91.351	0.389
pilT	Acinetobacter baylyi ADP1	42.059	91.892	0.386
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	40.634	93.784	0.381
pilT	Vibrio cholerae strain A1552	40.634	93.784	0.381
pilT	Legionella pneumophila strain Lp02	40.533	91.351	0.37
pilT	Legionella pneumophila strain ERS1305867	40.533	91.351	0.37
pilT	Neisseria meningitidis 8013	39.368	94.054	0.37
pilT	Neisseria gonorrhoeae MS11	39.368	94.054	0.37