Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   MNO12_RS18875 Genome accession   NZ_CP093334
Coordinates   3911384..3912364 (+) Length   326 a.a.
NCBI ID   WP_032690764.1    Uniprot ID   A0A8G2E9D1
Organism   Raoultella planticola strain A2-F21     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3906384..3917364
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MNO12_RS18845 (MNO12_18845) - 3906696..3907742 (-) 1047 WP_241950300.1 sugar ABC transporter substrate-binding protein -
  MNO12_RS18850 (MNO12_18850) hemW 3908054..3909193 (-) 1140 WP_064793592.1 radical SAM family heme chaperone HemW -
  MNO12_RS18855 (MNO12_18855) - 3909186..3909779 (-) 594 WP_032690759.1 XTP/dITP diphosphatase -
  MNO12_RS18860 (MNO12_18860) yggU 3909792..3910082 (-) 291 WP_032690761.1 DUF167 family protein YggU -
  MNO12_RS18865 (MNO12_18865) - 3910079..3910645 (-) 567 WP_241950301.1 YggT family protein -
  MNO12_RS18870 (MNO12_18870) - 3910663..3911367 (-) 705 WP_032697613.1 YggS family pyridoxal phosphate-dependent enzyme -
  MNO12_RS18875 (MNO12_18875) pilT 3911384..3912364 (+) 981 WP_032690764.1 type IV pilus twitching motility protein PilT Machinery gene
  MNO12_RS18880 (MNO12_18880) - 3912699..3913352 (+) 654 WP_032697615.1 LuxR C-terminal-related transcriptional regulator -
  MNO12_RS18885 (MNO12_18885) ruvX 3913422..3913838 (-) 417 WP_032698803.1 Holliday junction resolvase RuvX -
  MNO12_RS18890 (MNO12_18890) - 3913838..3914401 (-) 564 WP_015585751.1 YqgE/AlgH family protein -
  MNO12_RS18895 (MNO12_18895) gshB 3915019..3915966 (-) 948 WP_032697616.1 glutathione synthase -
  MNO12_RS18900 (MNO12_18900) rsmE 3915987..3916718 (-) 732 WP_032697617.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35596.78 Da        Isoelectric Point: 7.0928

>NTDB_id=663744 MNO12_RS18875 WP_032690764.1 3911384..3912364(+) (pilT) [Raoultella planticola strain A2-F21]
MELEEIVALSVKHNVSDLHLCNTSAARWRRQGKLEPAPFHTPDIGKWLDGWLDEKQRAHWQANGQVDFALTPDGGPRLRA
SAFSHARGISLALRLLPESCPRIEALRVPAALTELLNEESGLILVTGATGSGKSTTLAAMVNHLNQQREGHILTLEDPVE
FVHHSKRCLIQQREIGRDCPSFSTALRGALRQDPDVILLGELRDSETIRLALTAAETGHLVMATLHTRGAAQAIERLVDV
FPAQEKEQIRSQLAGSLCAVLAQKLLPTASEGRVALYELLVNTPAVANLIREGKTHQLPGVMQTGQQAGMLTFTQSFQQR
VAAGLL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=663744 MNO12_RS18875 WP_032690764.1 3911384..3912364(+) (pilT) [Raoultella planticola strain A2-F21]
ATGGAGCTGGAAGAAATCGTGGCCCTTAGTGTAAAGCATAATGTCTCCGATCTACACCTGTGCAATACCTCGGCAGCGCG
CTGGCGTCGACAGGGAAAGCTTGAGCCGGCGCCATTTCACACGCCGGATATCGGGAAATGGCTTGACGGGTGGCTCGATG
AGAAACAGCGCGCGCACTGGCAGGCCAACGGACAGGTGGATTTTGCCTTAACCCCCGACGGCGGACCGCGCCTTCGCGCC
AGCGCATTTTCTCACGCTCGGGGTATTTCATTAGCGCTGCGCCTGTTGCCGGAAAGCTGTCCCCGGATTGAGGCGCTGCG
TGTTCCTGCGGCGCTGACGGAACTGCTGAATGAAGAAAGCGGTTTGATTCTGGTGACCGGAGCCACCGGCAGCGGTAAGT
CGACCACCCTCGCGGCGATGGTCAATCATCTCAACCAGCAGCGTGAAGGGCATATTCTGACGCTTGAAGATCCGGTGGAG
TTTGTCCATCACAGCAAGCGATGTCTGATTCAACAACGTGAAATCGGACGCGACTGTCCCTCTTTTTCAACGGCGCTGCG
GGGGGCGCTGCGCCAGGATCCCGATGTGATTCTGCTGGGTGAGCTGCGCGACAGCGAAACGATCCGCCTGGCGCTGACCG
CTGCGGAGACCGGCCATCTGGTGATGGCCACGCTGCATACCCGCGGCGCGGCTCAGGCGATTGAGCGTCTGGTGGATGTC
TTCCCTGCGCAGGAAAAAGAACAAATACGCAGCCAGCTAGCGGGAAGTTTATGTGCGGTGCTGGCACAAAAATTACTGCC
GACAGCGTCAGAGGGAAGAGTTGCGCTATACGAGCTTTTGGTGAATACCCCGGCGGTAGCAAATTTGATTCGCGAGGGGA
AAACTCATCAATTGCCGGGGGTCATGCAAACCGGTCAACAAGCCGGAATGCTGACTTTTACGCAAAGTTTTCAACAGCGT
GTGGCAGCGGGTCTGTTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(72-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

49.544

100

0.5
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.544

100

0.5
  pilT Neisseria meningitidis 8013

47.576

100

0.482
  pilT Neisseria gonorrhoeae MS11

47.273

100

0.479
  pilT Pseudomonas stutzeri DSM 10701

46.667

100

0.472
  pilT Acinetobacter baumannii D1279779

46.364

100

0.469
  pilT Acinetobacter baumannii strain A118

46.364

100

0.469
  pilT Acinetobacter nosocomialis M2

46.061

100

0.466
  pilT Acinetobacter baylyi ADP1

45.758

100

0.463
  pilT Legionella pneumophila strain ERS1305867

45.897

100

0.463
  pilT Legionella pneumophila strain Lp02

45.897

100

0.463
  pilT Pseudomonas aeruginosa PAK

45.897

100

0.463
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.11

97.239

0.439
  pilU Vibrio cholerae strain A1552

41.667

99.387

0.414
  pilU Pseudomonas stutzeri DSM 10701

40.244

100

0.405
  pilU Acinetobacter baylyi ADP1

38.509

98.773

0.38