Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   MNO11_RS04020 Genome accession   NZ_CP093323
Coordinates   899389..900396 (+) Length   335 a.a.
NCBI ID   WP_241921700.1    Uniprot ID   -
Organism   Serratia plymuthica strain B37/06     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 894389..905396
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MNO11_RS03985 (MNO11_03985) - 894733..895167 (+) 435 WP_241921699.1 DUF29 domain-containing protein -
  MNO11_RS03990 (MNO11_03990) hemW 895207..896346 (-) 1140 WP_062868634.1 radical SAM family heme chaperone HemW -
  MNO11_RS03995 (MNO11_03995) - 896339..896932 (-) 594 WP_062792737.1 XTP/dITP diphosphatase -
  MNO11_RS04000 (MNO11_04000) yggU 896950..897240 (-) 291 WP_004948014.1 DUF167 family protein YggU -
  MNO11_RS04005 (MNO11_04005) - 897237..897791 (-) 555 WP_004948012.1 YggT family protein -
  MNO11_RS04010 (MNO11_04010) proC 897823..898641 (-) 819 WP_062868632.1 pyrroline-5-carboxylate reductase -
  MNO11_RS04015 (MNO11_04015) - 898661..899368 (-) 708 WP_166729338.1 YggS family pyridoxal phosphate-dependent enzyme -
  MNO11_RS04020 (MNO11_04020) pilT 899389..900396 (+) 1008 WP_241921700.1 type IV pilus twitching motility protein PilT Machinery gene
  MNO11_RS04025 (MNO11_04025) ruvX 900405..900827 (-) 423 WP_013814482.1 Holliday junction resolvase RuvX -
  MNO11_RS04030 (MNO11_04030) - 900827..901390 (-) 564 WP_004948000.1 YqgE/AlgH family protein -
  MNO11_RS04035 (MNO11_04035) gshB 901662..902612 (-) 951 WP_063200461.1 glutathione synthase -
  MNO11_RS04040 (MNO11_04040) rsmE 902623..903354 (-) 732 WP_013814479.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  MNO11_RS04045 (MNO11_04045) endA 903539..904234 (-) 696 WP_063200464.1 deoxyribonuclease I -
  MNO11_RS04050 (MNO11_04050) - 904336..904848 (-) 513 WP_004947987.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 335 a.a.        Molecular weight: 36317.72 Da        Isoelectric Point: 6.3658

>NTDB_id=663569 MNO11_RS04020 WP_241921700.1 899389..900396(+) (pilT) [Serratia plymuthica strain B37/06]
MDIDEFVALSVKHNASDLHLCAGHQPMLRIDGELQPLEGAEILTQERMQQLCGTLLQPPQRQLLQQHGQLDLALTLAGDI
RLRANFFLQSLGISIALRLIAGHCPTLAALASPAIVPALLRREDGLILVTGATGSGKSTTLAAMIDDINQHQRRHVITLE
DPIEFMHRSRKSLIQQRELGRDSHSFDEALRAALREDPDVILLGELRDTTTIRLALTAAETGHLVLATLHTRSAPQAVDR
LVDVFPAEEKPYVRAQLAASLQAVIAQKLLSRPGGGRVAIFEVLTATAAVSSMIREGKTHQLASVLQTGAQSGMQTFEQG
LQQRIDAGLLGGEEA

Nucleotide


Download         Length: 1008 bp        

>NTDB_id=663569 MNO11_RS04020 WP_241921700.1 899389..900396(+) (pilT) [Serratia plymuthica strain B37/06]
ATGGATATCGACGAATTCGTGGCCCTTAGTGTAAAGCATAATGCTTCCGATCTGCACCTTTGTGCGGGCCATCAGCCCAT
GTTGCGCATTGATGGTGAACTGCAACCGCTGGAAGGGGCGGAAATACTGACTCAGGAGCGAATGCAGCAGCTGTGCGGCA
CGCTGCTGCAACCGCCGCAGCGACAGCTTTTGCAGCAGCATGGGCAGCTCGATCTGGCGTTGACGTTGGCGGGCGATATC
CGGCTGCGCGCCAATTTCTTTTTGCAAAGCCTGGGGATATCCATCGCGCTGCGGTTGATCGCCGGCCACTGCCCGACGCT
GGCGGCGTTAGCGTCACCGGCCATTGTCCCCGCGCTGCTGCGGCGTGAGGATGGCCTGATATTGGTTACCGGCGCGACCG
GCAGCGGTAAGTCCACCACGCTGGCGGCGATGATTGACGACATCAATCAGCATCAACGCCGGCATGTCATCACGCTGGAA
GACCCGATCGAATTTATGCACCGCAGCCGAAAGTCGCTGATCCAACAGCGTGAGCTGGGCCGCGATAGCCACAGTTTTGA
CGAAGCGCTACGCGCCGCGCTGCGTGAAGATCCGGATGTGATCCTGTTGGGAGAACTGCGTGACACCACCACCATCCGTC
TGGCGCTCACTGCGGCGGAAACCGGCCATTTGGTGCTGGCGACGCTGCACACCCGCAGCGCACCGCAGGCGGTGGATCGT
TTGGTAGACGTTTTCCCGGCGGAAGAAAAGCCTTATGTGCGCGCACAGCTGGCTGCCAGCCTGCAGGCGGTGATTGCGCA
AAAGCTGCTAAGCAGGCCCGGCGGCGGGCGAGTGGCGATCTTCGAGGTGCTGACGGCAACGGCGGCGGTCAGCAGCATGA
TCCGCGAAGGCAAAACCCATCAGCTTGCCAGCGTGCTGCAAACCGGCGCGCAGTCCGGCATGCAGACCTTTGAACAGGGA
TTGCAGCAACGGATCGACGCCGGTTTGTTGGGGGGAGAAGAAGCGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

52.994

99.701

0.528
  pilT Vibrio cholerae strain A1552

52.994

99.701

0.528
  pilT Neisseria meningitidis 8013

49.405

100

0.496
  pilT Neisseria gonorrhoeae MS11

49.107

100

0.493
  pilT Acinetobacter baumannii strain A118

48.955

100

0.49
  pilT Acinetobacter nosocomialis M2

48.955

100

0.49
  pilT Acinetobacter baumannii D1279779

48.955

100

0.49
  pilT Acinetobacter baylyi ADP1

49.245

98.806

0.487
  pilT Pseudomonas aeruginosa PAK

47.941

100

0.487
  pilT Legionella pneumophila strain ERS1305867

47.059

100

0.478
  pilT Legionella pneumophila strain Lp02

47.059

100

0.478
  pilT Pseudomonas stutzeri DSM 10701

47.463

100

0.475
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.322

100

0.463
  pilU Acinetobacter baylyi ADP1

39.009

96.418

0.376
  pilU Pseudomonas stutzeri DSM 10701

37.202

100

0.373
  pilU Vibrio cholerae strain A1552

37.346

96.716

0.361