Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   N023_RS02330 Genome accession   NZ_CP093321
Coordinates   513620..514654 (+) Length   344 a.a.
NCBI ID   WP_003345166.1    Uniprot ID   A0A3M4E3M7
Organism   Pseudomonas syringae CC440     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 508620..519654
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N023_RS02300 (N023_02300) rdgB 508761..509354 (-) 594 WP_003366128.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -
  N023_RS02305 (N023_02305) metW 509388..510008 (-) 621 WP_024650313.1 methionine biosynthesis protein MetW -
  N023_RS02310 (N023_02310) - 510016..511155 (-) 1140 WP_003316725.1 homoserine O-acetyltransferase -
  N023_RS02315 (N023_02315) - 511422..512012 (-) 591 WP_003345162.1 YggT family protein -
  N023_RS02320 (N023_02320) proC 512038..512856 (-) 819 WP_003422450.1 pyrroline-5-carboxylate reductase -
  N023_RS02325 (N023_02325) - 512878..513564 (-) 687 WP_003422448.1 YggS family pyridoxal phosphate-dependent enzyme -
  N023_RS02330 (N023_02330) pilT 513620..514654 (+) 1035 WP_003345166.1 type IV pilus twitching motility protein PilT Machinery gene
  N023_RS02335 (N023_02335) - 514749..515426 (-) 678 WP_050586328.1 C40 family peptidase -
  N023_RS02340 (N023_02340) - 515677..516081 (+) 405 WP_003345171.1 TM2 domain-containing protein -
  N023_RS02345 (N023_02345) - 516359..517630 (-) 1272 WP_024665455.1 dihydroorotase -
  N023_RS02350 (N023_02350) - 517627..518631 (-) 1005 WP_003316734.1 aspartate carbamoyltransferase catalytic subunit -
  N023_RS02355 (N023_02355) pyrR 518644..519156 (-) 513 WP_003316735.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37965.82 Da        Isoelectric Point: 7.0226

>NTDB_id=663521 N023_RS02330 WP_003345166.1 513620..514654(+) (pilT) [Pseudomonas syringae CC440]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPPLDAKEVKALIYDIMNDKQRQDFEERLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKILSMEDLGMGNVFRKITDVARGLILVTGPTGSGKSTTLAAMIDYLNCNKHHHILTIED
PIEFVHESKKCLVNQREVHRDTLGFSEALRSALREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRI
VDVFPAQEKSMIRSMLSESLHAVVSQALLKKVGGGRVAAHEIMMGTPAIRNLIREDKVAQMYSSIQTGGSMGMQTLDMCL
ADLVKKGLITRESARERAKVPDNF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=663521 N023_RS02330 WP_003345166.1 513620..514654(+) (pilT) [Pseudomonas syringae CC440]
ATGGATATTACCGAGCTGCTGGCCTTCAGTGCCAAACAGGGCGCGTCGGACTTGCACCTCTCTGCAGGTCTGCCGCCGAT
GATCCGCGTCGACGGCGATGTACGGCGTATCAACCTGCCCCCGCTGGACGCGAAGGAGGTCAAGGCGCTGATCTACGACA
TCATGAACGACAAGCAACGGCAGGATTTCGAAGAGCGGCTGGAAACCGACTTTTCCTTCGAAGTGCCGGGCGTGGCGCGG
TTTCGGGTCAACGCCTTCAACCAGAATCGCGGTGCCGGCGCGGTATTCCGGACCATTCCCTCGAAAATCCTGAGCATGGA
AGACTTGGGCATGGGCAATGTGTTTCGCAAGATTACCGACGTGGCGCGAGGCCTGATCCTGGTCACCGGTCCGACCGGCT
CAGGCAAGTCGACCACTCTGGCGGCGATGATCGACTACCTCAATTGCAACAAGCATCACCATATCCTGACCATCGAGGAC
CCGATCGAATTCGTCCACGAGTCGAAGAAGTGCCTGGTCAACCAGCGCGAGGTGCACCGCGACACGCTGGGCTTCTCGGA
GGCGCTGCGCTCGGCGTTGCGTGAAGACCCGGACGTGATTCTGGTCGGTGAAATGCGTGACCTGGAGACCATCCGCCTGG
CGCTGACCGCTGCTGAGACCGGCCATCTGGTATTCGGCACGCTGCACACCACGTCAGCGGCCAAGACCATCGACCGGATC
GTCGACGTGTTTCCTGCTCAGGAAAAATCGATGATTCGCTCGATGCTCTCCGAATCGCTGCACGCCGTGGTCTCGCAGGC
GTTGCTCAAGAAGGTCGGCGGCGGACGCGTGGCGGCGCACGAAATCATGATGGGCACCCCGGCGATCCGTAACCTGATCC
GTGAGGACAAGGTGGCGCAGATGTATTCGTCGATCCAGACGGGCGGCTCGATGGGCATGCAGACGCTGGACATGTGCCTG
GCCGATCTGGTGAAGAAGGGGCTGATCACCCGCGAAAGCGCACGCGAACGCGCCAAGGTGCCGGATAACTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3M4E3M7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

89.244

100

0.892
  pilT Pseudomonas aeruginosa PAK

87.791

100

0.878
  pilT Acinetobacter nosocomialis M2

78.779

100

0.788
  pilT Acinetobacter baumannii D1279779

78.779

100

0.788
  pilT Acinetobacter baumannii strain A118

78.779

100

0.788
  pilT Acinetobacter baylyi ADP1

78.198

100

0.782
  pilT Legionella pneumophila strain Lp02

74.26

98.256

0.73
  pilT Legionella pneumophila strain ERS1305867

74.26

98.256

0.73
  pilT Neisseria meningitidis 8013

67.941

98.837

0.672
  pilT Neisseria gonorrhoeae MS11

67.647

98.837

0.669
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

66.766

97.965

0.654
  pilT Vibrio cholerae strain A1552

66.766

97.965

0.654
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.333

100

0.535
  pilU Acinetobacter baylyi ADP1

41.04

100

0.413
  pilU Pseudomonas stutzeri DSM 10701

42.09

97.384

0.41
  pilU Vibrio cholerae strain A1552

40.299

97.384

0.392