Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   MN086_RS02125 Genome accession   NZ_CP093312
Coordinates   418364..419422 (-) Length   352 a.a.
NCBI ID   WP_248576415.1    Uniprot ID   -
Organism   Sulfurovum sp. XGS-02     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 413364..424422
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MN086_RS02100 (MN086_02100) - 413613..413765 (-) 153 WP_248576410.1 hypothetical protein -
  MN086_RS11055 - 413925..414107 (-) 183 Protein_408 DUF1882 domain-containing protein -
  MN086_RS02105 (MN086_02105) - 414117..415367 (-) 1251 WP_248576411.1 serine hydroxymethyltransferase -
  MN086_RS02110 (MN086_02110) lysS 415412..417067 (-) 1656 WP_248576412.1 lysine--tRNA ligase -
  MN086_RS02115 (MN086_02115) - 417197..417634 (-) 438 WP_248576413.1 Fur family transcriptional regulator -
  MN086_RS02120 (MN086_02120) - 417643..418269 (-) 627 WP_248576414.1 CvpA family protein -
  MN086_RS02125 (MN086_02125) pilT 418364..419422 (-) 1059 WP_248576415.1 type IV pilus twitching motility protein PilT Machinery gene
  MN086_RS02130 (MN086_02130) - 419436..419777 (-) 342 WP_248576416.1 arsenate reductase family protein -
  MN086_RS02135 (MN086_02135) gatC 419787..420077 (-) 291 WP_248576417.1 Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatC -
  MN086_RS02140 (MN086_02140) - 420179..420520 (+) 342 WP_248576418.1 ferredoxin-thioredoxin reductase catalytic domain-containing protein -
  MN086_RS02145 (MN086_02145) - 420603..420947 (+) 345 WP_248576419.1 rhodanese-like domain-containing protein -
  MN086_RS02150 (MN086_02150) - 420949..421497 (+) 549 WP_248576420.1 YqiA/YcfP family alpha/beta fold hydrolase -
  MN086_RS02155 (MN086_02155) dapE 421504..422613 (+) 1110 WP_248576421.1 succinyl-diaminopimelate desuccinylase -
  MN086_RS02160 (MN086_02160) - 422610..422993 (+) 384 WP_248576422.1 thioredoxin family protein -
  MN086_RS02165 (MN086_02165) - 423000..423497 (+) 498 WP_248576423.1 thioredoxin family protein -

Sequence

Protein


Download         Length: 352 a.a.        Molecular weight: 38488.14 Da        Isoelectric Point: 6.1304

>NTDB_id=663479 MN086_RS02125 WP_248576415.1 418364..419422(-) (pilT) [Sulfurovum sp. XGS-02]
MAAFDIKKLLQSVVTHGASDLHLVSRTEPQIRLDGVLKPVNLPALTGEDIEEMCYSLITEKQKQQFEEHDELDFALLLPG
IGRFRANYYRTLGDTAAAFRIIPIDVPSLDDLGAPEVYKSLIKREKGLILVTGPTGSGKSTTLAAMLNEINLHEQKHIVT
VEDPVEFIHQNKKSVFSHRGVGEDTASFATALKYAMRQDPDIILIGEMRDKETIEAGLTAAETGHLVFGTLHTSSAPGTI
NRIIDVFTGDEQPQIRAMIASSLVAVIAQALLPKLGGGRVAASEILVTNHAISNLIREDKVHQIYSQMQLGQGDTGMQTQ
TQALLKFLKDGKISRDVAMQYANRPAELSGKI

Nucleotide


Download         Length: 1059 bp        

>NTDB_id=663479 MN086_RS02125 WP_248576415.1 418364..419422(-) (pilT) [Sulfurovum sp. XGS-02]
ATGGCTGCTTTTGATATCAAAAAACTGCTTCAGAGTGTTGTTACACATGGTGCGTCTGACCTCCACCTCGTAAGTCGGAC
AGAACCGCAGATAAGACTCGACGGTGTACTTAAACCTGTTAATCTTCCTGCACTCACAGGGGAGGATATCGAGGAGATGT
GTTACTCTCTTATCACAGAGAAACAGAAACAACAATTTGAAGAGCATGATGAACTGGACTTCGCACTCTTGCTTCCTGGT
ATCGGGCGATTCAGGGCAAACTACTATCGTACATTAGGGGACACTGCCGCTGCATTCAGGATCATTCCTATTGATGTACC
TTCTTTGGATGATCTGGGTGCGCCTGAAGTCTATAAAAGTCTTATCAAGAGAGAAAAAGGGCTTATCTTGGTCACAGGGC
CGACAGGTTCTGGTAAATCAACCACACTGGCTGCCATGCTCAATGAGATCAACCTTCATGAACAAAAACACATCGTTACC
GTGGAAGATCCAGTGGAATTTATCCACCAGAACAAAAAATCTGTCTTTTCTCACAGAGGTGTAGGTGAAGATACTGCTTC
TTTTGCTACAGCACTGAAATATGCCATGCGTCAAGACCCCGACATCATACTGATCGGGGAGATGAGGGATAAGGAAACCA
TTGAAGCCGGGCTTACTGCGGCTGAAACCGGTCACCTTGTATTCGGTACCCTGCATACCTCTTCGGCACCGGGAACCATT
AACCGTATCATCGATGTATTTACAGGGGATGAACAACCGCAGATTAGAGCCATGATTGCCTCTTCACTGGTGGCTGTTAT
CGCACAGGCGCTTCTCCCTAAGTTGGGTGGAGGCCGTGTCGCTGCTTCTGAGATCTTGGTAACCAATCACGCGATTTCAA
ACCTCATTCGTGAAGATAAGGTACACCAGATCTATTCACAAATGCAACTGGGACAAGGTGATACAGGTATGCAGACGCAG
ACACAGGCACTGCTTAAATTCCTCAAAGATGGGAAGATAAGCCGTGACGTTGCTATGCAGTATGCCAACAGACCGGCTGA
ACTTTCTGGAAAAATATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-274)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

51.754

97.159

0.503
  pilT Acinetobacter baumannii D1279779

51.17

97.159

0.497
  pilT Acinetobacter nosocomialis M2

51.17

97.159

0.497
  pilT Acinetobacter baumannii strain A118

51.17

97.159

0.497
  pilT Pseudomonas aeruginosa PAK

50.292

97.159

0.489
  pilT Acinetobacter baylyi ADP1

50.292

97.159

0.489
  pilT Legionella pneumophila strain ERS1305867

50.296

96.023

0.483
  pilT Legionella pneumophila strain Lp02

50.296

96.023

0.483
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

51.515

93.75

0.483
  pilT Vibrio cholerae strain A1552

51.515

93.75

0.483
  pilT Neisseria meningitidis 8013

47.839

98.58

0.472
  pilT Neisseria gonorrhoeae MS11

47.839

98.58

0.472
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.845

99.148

0.455
  pilU Acinetobacter baylyi ADP1

39.429

99.432

0.392
  pilU Pseudomonas stutzeri DSM 10701

38.15

98.295

0.375
  pilU Vibrio cholerae strain A1552

37.572

98.295

0.369