Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   LIW09_RS11210 Genome accession   NZ_CP093311
Coordinates   2390535..2391641 (+) Length   368 a.a.
NCBI ID   WP_256645701.1    Uniprot ID   -
Organism   Thermomonas paludicola strain IMCC34681     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2385535..2396641
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LIW09_RS11185 - 2385678..2387000 (-) 1323 WP_256645697.1 ABC transporter ATP-binding protein -
  LIW09_RS11190 - 2386990..2387802 (-) 813 WP_256645698.1 ABC transporter permease -
  LIW09_RS11195 - 2387834..2388232 (-) 399 Protein_2184 CBS domain-containing protein -
  LIW09_RS11200 - 2388546..2388761 (-) 216 WP_256645699.1 YdcH family protein -
  LIW09_RS11205 - 2389182..2390444 (-) 1263 WP_256645700.1 OmpA family protein -
  LIW09_RS11210 pilU 2390535..2391641 (+) 1107 WP_256645701.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  LIW09_RS11215 maiA 2391664..2392362 (-) 699 WP_256645702.1 maleylacetoacetate isomerase -
  LIW09_RS11220 - 2392442..2393431 (-) 990 WP_256645703.1 fumarylacetoacetate hydrolase family protein -
  LIW09_RS11225 - 2393494..2394237 (-) 744 WP_256645704.1 2OG-Fe dioxygenase family protein -
  LIW09_RS11235 rpsI 2394548..2394940 (-) 393 WP_256645705.1 30S ribosomal protein S9 -
  LIW09_RS11240 rplM 2394943..2395371 (-) 429 WP_256645706.1 50S ribosomal protein L13 -
  LIW09_RS11245 coq7 2395552..2396196 (+) 645 WP_256645707.1 2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinone monooxygenase -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 40729.92 Da        Isoelectric Point: 6.0155

>NTDB_id=663474 LIW09_RS11210 WP_256645701.1 2390535..2391641(+) (pilU) [Thermomonas paludicola strain IMCC34681]
MDITHFFKLMADKDASDMFLSTGAPVNIKIEGVLQPMSDKPLTAGECRDIAYGLMDTAQVAEFEREKELNMAISVPGSGR
FRVNVFQQRGEVGMVIRTIRSIIPSIEELQLPLVLKDIIMAPRGLVLIVGSTGSGKSTTLASMIDHRNSTSTGHILTIED
PIEYLHRHKLSVVNQREVGLDTHSFHAALKNAMREAPDVILIGEIRDAQTMEAAIAFAETGHICLATLHSNNADQTIERI
LNFFPEGAHKNVLMNLALNLKAVISQRLVLGMDGRRMPATEVLINTPMIRDLLRRGQVHEIKMAMEDSLQEGMQSFDQCL
FRLHKDGRIELEEALKAADSRDGLALKFRLSEGAAGDHDPYADVFATH

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=663474 LIW09_RS11210 WP_256645701.1 2390535..2391641(+) (pilU) [Thermomonas paludicola strain IMCC34681]
ATGGATATCACCCACTTCTTCAAGCTGATGGCGGACAAGGACGCCTCGGACATGTTCCTGTCCACCGGTGCGCCGGTGAA
CATCAAGATCGAAGGCGTGCTGCAGCCGATGAGCGACAAGCCGCTCACCGCCGGCGAATGCCGGGACATCGCCTACGGGC
TGATGGACACGGCGCAGGTGGCCGAGTTCGAGCGCGAGAAAGAGCTGAACATGGCGATCTCGGTGCCCGGCAGCGGCCGC
TTCCGCGTCAACGTGTTCCAGCAGCGCGGCGAAGTCGGCATGGTGATCCGTACCATCCGCAGCATCATCCCCAGCATCGA
GGAACTGCAGCTGCCGCTGGTGCTGAAGGACATCATCATGGCGCCGCGCGGGCTGGTGCTGATCGTCGGTTCAACCGGCT
CCGGCAAGTCCACCACGCTGGCGTCGATGATCGACCATCGCAACAGCACCAGCACCGGTCATATCCTGACCATCGAAGAC
CCGATCGAATACCTGCACCGGCACAAGCTGTCGGTGGTCAACCAGCGCGAGGTCGGGCTGGACACGCACAGCTTCCACGC
CGCCCTGAAGAACGCGATGCGCGAGGCGCCGGACGTGATCCTGATCGGCGAAATCCGCGACGCGCAGACGATGGAGGCGG
CGATCGCGTTCGCCGAAACCGGCCACATCTGCCTGGCCACGCTGCACTCCAACAACGCCGACCAGACCATCGAGCGCATT
CTCAATTTCTTCCCCGAAGGTGCGCACAAGAACGTGCTGATGAACCTCGCGCTGAACCTCAAGGCGGTGATCAGCCAGCG
CCTGGTGCTGGGCATGGACGGCCGCCGCATGCCGGCCACCGAGGTGCTGATCAACACGCCGATGATCCGCGACCTGCTGC
GTCGCGGGCAGGTGCACGAAATCAAGATGGCCATGGAAGACTCGCTGCAGGAAGGCATGCAGAGTTTCGACCAGTGCCTG
TTCCGGCTGCACAAGGACGGCAGGATCGAGCTCGAGGAGGCATTGAAGGCGGCCGACTCGCGCGATGGCCTGGCGCTCAA
ATTCCGCCTGTCGGAAGGCGCCGCCGGCGATCACGACCCTTACGCGGACGTGTTCGCCACGCACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(47-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

50.696

97.554

0.495
  pilU Acinetobacter baylyi ADP1

49.719

96.739

0.481
  pilU Vibrio cholerae strain A1552

48.87

96.196

0.47
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.251

97.283

0.44
  pilT Pseudomonas aeruginosa PAK

43.491

91.848

0.399
  pilT Pseudomonas stutzeri DSM 10701

42.308

91.848

0.389
  pilT Acinetobacter baylyi ADP1

42.059

92.391

0.389
  pilT Acinetobacter baumannii D1279779

41.42

91.848

0.38
  pilT Acinetobacter nosocomialis M2

41.42

91.848

0.38
  pilT Acinetobacter baumannii strain A118

41.42

91.848

0.38
  pilT Legionella pneumophila strain Lp02

41.124

91.848

0.378
  pilT Legionella pneumophila strain ERS1305867

41.124

91.848

0.378
  pilT Neisseria gonorrhoeae MS11

39.769

94.293

0.375
  pilT Neisseria meningitidis 8013

39.769

94.293

0.375
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

39.82

90.761

0.361
  pilT Vibrio cholerae strain A1552

39.82

90.761

0.361