Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   PST29_RS28490 Genome accession   NZ_AP014628
Coordinates   6382150..6383184 (-) Length   344 a.a.
NCBI ID   WP_060844874.1    Uniprot ID   A0AAD1BFE4
Organism   Pseudomonas sp. St29     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6377150..6388184
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PST29_RS28460 (PST29_5783) ruvX 6377282..6377719 (+) 438 WP_060841532.1 Holliday junction resolvase RuvX -
  PST29_RS28465 (PST29_5784) pyrR 6377837..6378343 (+) 507 WP_060841533.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  PST29_RS28470 (PST29_5785) - 6378369..6379373 (+) 1005 WP_011064007.1 aspartate carbamoyltransferase catalytic subunit -
  PST29_RS28475 (PST29_5786) - 6379370..6380641 (+) 1272 WP_060844873.1 dihydroorotase -
  PST29_RS28480 (PST29_5787) - 6380769..6381188 (-) 420 WP_060841535.1 TM2 domain-containing protein -
  PST29_RS28485 (PST29_5788) - 6381440..6382063 (+) 624 WP_060841536.1 C40 family peptidase -
  PST29_RS28490 (PST29_5789) pilT 6382150..6383184 (-) 1035 WP_060844874.1 type IV pilus twitching motility protein PilT Machinery gene
  PST29_RS28495 (PST29_5790) - 6383378..6384076 (+) 699 WP_060844875.1 YggS family pyridoxal phosphate-dependent enzyme -
  PST29_RS28500 (PST29_5791) proC 6384111..6384929 (+) 819 WP_060844876.1 pyrroline-5-carboxylate reductase -
  PST29_RS28505 (PST29_5792) - 6384940..6385530 (+) 591 WP_060844877.1 YggT family protein -
  PST29_RS28510 (PST29_5793) - 6385545..6385838 (+) 294 WP_060841541.1 DUF167 domain-containing protein -
  PST29_RS28515 (PST29_5794) - 6386012..6387151 (+) 1140 WP_060841542.1 homoserine O-acetyltransferase -
  PST29_RS28520 (PST29_5795) metW 6387159..6387779 (+) 621 WP_060841543.1 methionine biosynthesis protein MetW -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37233.96 Da        Isoelectric Point: 6.8738

>NTDB_id=66343 PST29_RS28490 WP_060844874.1 6382150..6383184(-) (pilT) [Pseudomonas sp. St29]
MDITELLLLSVARGASDLHLSAGLAPMLRIDGDIGALEGPALDGQQTLALIHSLMSESQRQAFAVTRDLDFAYELPGVAR
FRVNAFQHARGVGAVLRLIPARVQSLEALGLGEVFRHIAEVPRGLVLVTGPTGSGKSTTLAAMIDHLNHHRRQHILTIED
PIEFVHPQHSCLVHQREVQRDTQSFASALRAALREDPDVILLGELRDLETIRLALTAAETGHLVLATLHTASAAKTIDRL
VDVFPGEEKALVRSMLAESLQAVVAQVLLKKVGGGRVAAHEIMLGSSAIRNLIREDKVAQMYSAIQTGGAQGMRTLDMSL
KALLGQGLISREQAREQARMPESF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=66343 PST29_RS28490 WP_060844874.1 6382150..6383184(-) (pilT) [Pseudomonas sp. St29]
ATGGACATCACCGAACTCTTGCTCTTGAGCGTCGCCCGAGGTGCTTCCGACCTGCATCTGTCCGCCGGACTGGCGCCGAT
GTTGCGTATCGACGGCGATATCGGCGCGCTGGAGGGGCCGGCCCTGGACGGGCAGCAGACGCTGGCCTTGATCCACAGCC
TGATGAGCGAATCGCAGCGCCAAGCCTTCGCCGTCACCCGGGACCTGGACTTCGCCTACGAGCTGCCGGGCGTGGCGCGC
TTTCGGGTCAACGCCTTCCAGCATGCCCGGGGCGTCGGCGCGGTGCTGCGCCTGATTCCTGCGCGGGTACAGAGCCTGGA
AGCGTTGGGGCTGGGGGAAGTGTTTCGGCACATTGCCGAGGTGCCCCGGGGGCTGGTGCTGGTGACCGGGCCCACCGGCA
GCGGCAAGTCCACCACCCTGGCGGCGATGATCGATCATCTGAACCACCATCGGCGCCAGCACATCCTCACCATCGAAGAT
CCCATCGAGTTCGTGCATCCGCAGCACAGCTGCCTGGTGCACCAGCGCGAGGTGCAGCGTGATACCCAGAGCTTCGCCAG
CGCCTTGCGCGCCGCGCTGCGGGAAGATCCGGACGTGATCCTGCTGGGAGAGCTGCGGGACCTGGAAACCATCCGCCTGG
CGCTGACCGCCGCAGAAACCGGGCATCTGGTACTGGCCACCCTGCACACCGCTTCGGCGGCCAAGACCATCGATCGGTTG
GTGGACGTGTTCCCGGGGGAGGAAAAGGCCCTGGTGCGCTCGATGCTGGCCGAGTCGCTGCAGGCGGTGGTTGCCCAGGT
GCTGCTGAAAAAGGTCGGTGGTGGCCGGGTGGCGGCGCACGAGATCATGCTGGGCAGCTCGGCGATTCGTAACCTGATTC
GCGAGGACAAGGTGGCGCAGATGTATTCGGCGATCCAGACTGGCGGCGCCCAGGGCATGCGCACTCTGGACATGAGCCTC
AAGGCGTTGCTCGGGCAGGGGTTGATCAGTCGCGAGCAGGCCCGGGAACAGGCGCGGATGCCGGAGAGTTTTTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

69.767

100

0.698
  pilT Pseudomonas aeruginosa PAK

69.477

100

0.695
  pilT Acinetobacter baylyi ADP1

65.407

100

0.654
  pilT Acinetobacter nosocomialis M2

65.116

100

0.651
  pilT Acinetobacter baumannii D1279779

65.116

100

0.651
  pilT Acinetobacter baumannii strain A118

65.116

100

0.651
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

62.315

97.965

0.61
  pilT Vibrio cholerae strain A1552

62.315

97.965

0.61
  pilT Legionella pneumophila strain Lp02

61.538

98.256

0.605
  pilT Legionella pneumophila strain ERS1305867

61.538

98.256

0.605
  pilT Neisseria meningitidis 8013

57.681

100

0.578
  pilT Neisseria gonorrhoeae MS11

57.391

100

0.576
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.464

100

0.526
  pilU Vibrio cholerae strain A1552

41.493

97.384

0.404
  pilU Pseudomonas stutzeri DSM 10701

40.896

97.384

0.398
  pilU Acinetobacter baylyi ADP1

39.13

100

0.392

Multiple sequence alignment