Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   MM271_RS19260 Genome accession   NZ_CP093300
Coordinates   3888818..3889858 (-) Length   346 a.a.
NCBI ID   WP_279390769.1    Uniprot ID   -
Organism   Alkalihalobacillus sp. LMS39     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3883818..3894858
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MM271_RS19225 (MM271_19225) - 3884071..3884655 (-) 585 WP_243529052.1 PilN domain-containing protein -
  MM271_RS19230 (MM271_19230) pilM 3884656..3885648 (-) 993 WP_243529054.1 pilus assembly protein PilM -
  MM271_RS19235 (MM271_19235) - 3885682..3886434 (-) 753 WP_243529055.1 A24 family peptidase -
  MM271_RS19240 (MM271_19240) - 3886466..3886786 (-) 321 WP_243529057.1 hypothetical protein -
  MM271_RS23930 - 3886883..3886987 (-) 105 Protein_3742 type II secretion system protein -
  MM271_RS19250 (MM271_19250) - 3887030..3887470 (-) 441 WP_243529059.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  MM271_RS19255 (MM271_19255) - 3887607..3888818 (-) 1212 WP_243529061.1 type II secretion system F family protein -
  MM271_RS19260 (MM271_19260) pilT 3888818..3889858 (-) 1041 WP_279390769.1 type IV pilus twitching motility protein PilT Machinery gene
  MM271_RS19265 (MM271_19265) gspE 3889878..3891533 (-) 1656 WP_243529063.1 type II secretion system ATPase GspE -
  MM271_RS19270 (MM271_19270) - 3891551..3892879 (-) 1329 WP_243529065.1 VanW family protein -
  MM271_RS19275 (MM271_19275) - 3892908..3893249 (-) 342 WP_243529067.1 hypothetical protein -
  MM271_RS19280 (MM271_19280) - 3893540..3894049 (+) 510 WP_243529068.1 type II secretion system protein -
  MM271_RS19285 (MM271_19285) - 3894000..3894557 (+) 558 WP_243529070.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -

Sequence

Protein


Download         Length: 346 a.a.        Molecular weight: 38555.27 Da        Isoelectric Point: 6.6202

>NTDB_id=663292 MM271_RS19260 WP_279390769.1 3888818..3889858(-) (pilT) [Alkalihalobacillus sp. LMS39]
MSYDINELLTMACEANASDLHVTIGAPPIVRLHGSLKQYGSEPLRPEHVHEMAKTLIPESQWNLFIEKGELDFSYGIPNV
SRFRLNVYHQRSHIGIVARVIPSTIPSLEQLQMPSLLKDLVEKPQGLILVTGPTGSGKSTTLAAMIDYVNQRDSKHIVTL
EDPIEYLHKHSRSIINQREIGFDTQSFSTGLRAALRQDPDIILVGEMRDLETISTAITAAETGHLVLATLHTSSAPQTID
RIIDVFPPHQQPQIRIQLASVLVGIISQRLFPTQSGKGRVAATEILINQPSVANLIRSEKNHQIKSIMQTSKAQGMHTLE
MSMKQLYQQGEIDMDIVKPYLEAGEW

Nucleotide


Download         Length: 1041 bp        

>NTDB_id=663292 MM271_RS19260 WP_279390769.1 3888818..3889858(-) (pilT) [Alkalihalobacillus sp. LMS39]
GTGAGCTACGATATCAATGAACTTCTTACGATGGCGTGTGAAGCAAATGCGTCTGACTTACATGTAACGATAGGGGCACC
ACCGATTGTTCGTCTTCATGGATCATTAAAGCAATATGGGAGTGAACCGCTGCGTCCTGAACATGTTCATGAGATGGCGA
AAACATTAATCCCTGAAAGTCAGTGGAATCTTTTTATAGAAAAAGGCGAATTAGACTTTTCATATGGCATTCCAAATGTG
TCTAGGTTTCGTTTAAATGTCTATCATCAGCGCAGTCATATTGGCATTGTCGCTCGTGTCATTCCATCGACAATTCCAAG
CTTAGAACAATTGCAAATGCCGTCTTTATTAAAAGATTTAGTAGAGAAACCACAAGGGTTAATTTTAGTAACTGGACCAA
CAGGCTCAGGGAAATCTACGACTCTAGCAGCGATGATAGATTATGTGAATCAACGAGATTCAAAACATATTGTGACATTA
GAAGACCCGATTGAGTATTTGCATAAACATAGTCGCTCGATTATTAATCAACGAGAAATTGGATTTGATACACAAAGCTT
TTCAACCGGATTGCGTGCAGCGTTACGACAAGACCCTGACATTATATTAGTTGGAGAAATGCGAGATTTAGAAACGATTT
CTACGGCAATCACTGCAGCGGAAACAGGTCATCTCGTGTTAGCAACCCTTCATACAAGCAGTGCACCACAAACAATTGAC
CGGATTATTGATGTGTTTCCTCCGCATCAACAGCCACAAATTCGAATTCAATTAGCATCTGTGTTAGTTGGTATCATTTC
TCAGCGGTTATTTCCAACGCAGTCGGGAAAAGGACGAGTGGCTGCGACAGAAATTTTAATTAATCAACCTTCTGTTGCGA
ATTTGATCCGAAGTGAAAAAAATCATCAAATTAAAAGCATTATGCAAACGAGCAAAGCACAAGGCATGCATACGCTTGAA
ATGTCGATGAAGCAATTGTATCAACAAGGCGAAATCGATATGGACATCGTCAAACCGTATCTAGAAGCAGGTGAGTGGTA
A

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(108-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.147

97.977

0.491
  pilT Vibrio cholerae strain A1552

50.147

97.977

0.491
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.301

95.954

0.483
  pilT Acinetobacter baylyi ADP1

49.849

95.665

0.477
  pilT Acinetobacter nosocomialis M2

49.544

95.087

0.471
  pilT Acinetobacter baumannii D1279779

49.544

95.087

0.471
  pilT Acinetobacter baumannii strain A118

49.544

95.087

0.471
  pilT Pseudomonas aeruginosa PAK

49.544

95.087

0.471
  pilT Neisseria meningitidis 8013

47.507

98.555

0.468
  pilT Pseudomonas stutzeri DSM 10701

48.06

96.821

0.465
  pilT Neisseria gonorrhoeae MS11

48.78

94.798

0.462
  pilT Legionella pneumophila strain Lp02

47.112

95.087

0.448
  pilT Legionella pneumophila strain ERS1305867

47.112

95.087

0.448
  pilU Pseudomonas stutzeri DSM 10701

43.465

95.087

0.413
  pilU Acinetobacter baylyi ADP1

40.303

95.376

0.384
  pilU Vibrio cholerae strain A1552

41.176

93.353

0.384