Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   POS17_RS28590 Genome accession   NZ_AP014627
Coordinates   6449601..6450635 (-) Length   344 a.a.
NCBI ID   WP_060841537.1    Uniprot ID   -
Organism   Pseudomonas sp. Os17     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6444601..6455635
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  POS17_RS28560 (POS17_5777) ruvX 6444733..6445170 (+) 438 WP_060841532.1 Holliday junction resolvase RuvX -
  POS17_RS28565 (POS17_5778) pyrR 6445288..6445794 (+) 507 WP_060841533.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  POS17_RS28570 (POS17_5779) - 6445820..6446824 (+) 1005 WP_011064007.1 aspartate carbamoyltransferase catalytic subunit -
  POS17_RS28575 (POS17_5780) - 6446821..6448092 (+) 1272 WP_060841534.1 dihydroorotase -
  POS17_RS28580 (POS17_5781) - 6448220..6448639 (-) 420 WP_060841535.1 TM2 domain-containing protein -
  POS17_RS28585 (POS17_5782) - 6448891..6449514 (+) 624 WP_060841536.1 C40 family peptidase -
  POS17_RS28590 (POS17_5783) pilT 6449601..6450635 (-) 1035 WP_060841537.1 type IV pilus twitching motility protein PilT Machinery gene
  POS17_RS28595 (POS17_5784) - 6450829..6451527 (+) 699 WP_060841538.1 YggS family pyridoxal phosphate-dependent enzyme -
  POS17_RS28600 (POS17_5785) proC 6451562..6452380 (+) 819 WP_060841539.1 pyrroline-5-carboxylate reductase -
  POS17_RS28605 (POS17_5786) - 6452391..6452981 (+) 591 WP_060841540.1 YggT family protein -
  POS17_RS28610 (POS17_5787) - 6452996..6453289 (+) 294 WP_060841541.1 DUF167 domain-containing protein -
  POS17_RS28615 (POS17_5788) - 6453464..6454603 (+) 1140 WP_060841542.1 homoserine O-acetyltransferase -
  POS17_RS28620 (POS17_5789) metW 6454611..6455231 (+) 621 WP_060841543.1 methionine biosynthesis protein MetW -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37235.00 Da        Isoelectric Point: 6.9822

>NTDB_id=66319 POS17_RS28590 WP_060841537.1 6449601..6450635(-) (pilT) [Pseudomonas sp. Os17]
MDITELLLLSVARGASDLHLSAGLAPMLRIDGDIGALEGPALDGQQTLALIHSLMSESQRQAFAVTRDLDFAYELPGVAR
FRVNAFQHARGAGAVLRLIPARVQSLEALGLGEVFRHIAEVPRGLVLVTGPTGSGKSTTLAAMIDHLNRHRRQHILTIED
PIEFVHPQHSCLVHQREVQRDTQSFASALRAALREDPDVILLGELRDLETIRLALTAAETGHLVLATLHTASAAKTIDRL
VDVFPGEEKALVRSMLAESLQAVVAQVLLKKVGGGRVAAHEIMLGSPAIRNLIREDKVAQMYSAIQTGGAQGMRTLDMSL
KALLGQGLISREQAREQARMPESF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=66319 POS17_RS28590 WP_060841537.1 6449601..6450635(-) (pilT) [Pseudomonas sp. Os17]
ATGGACATCACCGAACTCTTGCTCTTGAGCGTCGCCCGAGGTGCTTCCGACCTGCATCTGTCCGCCGGACTGGCGCCGAT
GTTGCGTATCGACGGCGATATCGGCGCGCTGGAGGGGCCGGCCCTGGACGGGCAGCAGACGCTGGCCTTGATCCACAGCC
TGATGAGCGAATCGCAGCGCCAAGCCTTCGCCGTCACCCGGGACCTGGACTTCGCCTACGAGCTGCCGGGCGTGGCGCGC
TTTCGGGTCAACGCCTTCCAGCATGCCCGGGGTGCCGGCGCGGTGCTGCGCCTGATTCCTGCGCGGGTACAGAGCCTGGA
AGCGTTGGGGCTGGGGGAAGTGTTTCGGCACATTGCCGAGGTGCCCCGGGGGCTGGTGCTGGTGACCGGGCCCACCGGCA
GCGGCAAGTCCACCACCCTGGCGGCGATGATCGATCATCTGAACCGCCATCGGCGCCAGCACATCCTCACCATCGAAGAT
CCCATCGAGTTCGTGCATCCGCAGCACAGCTGCCTGGTGCACCAGCGCGAGGTGCAGCGCGATACCCAGAGCTTCGCCAG
CGCCTTGCGCGCCGCGCTGCGGGAAGATCCGGACGTGATCCTGCTGGGAGAGCTGCGGGACCTGGAAACCATCCGCCTGG
CGCTGACCGCCGCAGAAACCGGGCATCTGGTACTGGCCACTCTGCACACCGCTTCGGCGGCCAAGACCATCGATCGGTTG
GTGGACGTGTTCCCGGGGGAGGAAAAGGCCCTGGTGCGCTCGATGCTGGCCGAGTCGCTGCAGGCGGTGGTTGCCCAGGT
GCTGCTGAAGAAGGTCGGTGGTGGCCGGGTGGCGGCGCACGAGATCATGCTGGGCAGCCCGGCGATTCGTAACCTGATTC
GCGAGGACAAGGTGGCGCAGATGTATTCGGCGATCCAGACTGGCGGCGCCCAGGGCATGCGCACTCTGGACATGAGCCTC
AAGGCGTTGCTCGGGCAGGGGTTGATCAGCCGCGAGCAGGCTCGGGAACAGGCGCGGATGCCGGAGAGCTTTTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

70.349

100

0.703
  pilT Pseudomonas aeruginosa PAK

70.058

100

0.701
  pilT Acinetobacter baylyi ADP1

65.988

100

0.66
  pilT Acinetobacter baumannii D1279779

65.698

100

0.657
  pilT Acinetobacter baumannii strain A118

65.698

100

0.657
  pilT Acinetobacter nosocomialis M2

65.698

100

0.657
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

62.611

97.965

0.613
  pilT Vibrio cholerae strain A1552

62.611

97.965

0.613
  pilT Legionella pneumophila strain Lp02

61.834

98.256

0.608
  pilT Legionella pneumophila strain ERS1305867

61.834

98.256

0.608
  pilT Neisseria meningitidis 8013

57.971

100

0.581
  pilT Neisseria gonorrhoeae MS11

57.681

100

0.578
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.464

100

0.526
  pilU Vibrio cholerae strain A1552

41.791

97.384

0.407
  pilU Pseudomonas stutzeri DSM 10701

41.493

97.384

0.404
  pilU Acinetobacter baylyi ADP1

39.42

100

0.395

Multiple sequence alignment