Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   MMW58_RS04215 Genome accession   NZ_CP093276
Coordinates   857752..858732 (+) Length   326 a.a.
NCBI ID   WP_159237127.1    Uniprot ID   -
Organism   Raoultella sp. HC6     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 852752..863732
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MMW58_RS04185 - 853049..854095 (-) 1047 WP_159237124.1 sugar ABC transporter substrate-binding protein -
  MMW58_RS04190 hemW 854408..855547 (-) 1140 WP_133600960.1 radical SAM family heme chaperone HemW -
  MMW58_RS04195 - 855540..856133 (-) 594 WP_045857059.1 XTP/dITP diphosphatase -
  MMW58_RS04200 yggU 856160..856450 (-) 291 WP_041147205.1 DUF167 family protein YggU -
  MMW58_RS04205 - 856447..857013 (-) 567 WP_041147204.1 YggT family protein -
  MMW58_RS04210 - 857031..857735 (-) 705 WP_133600961.1 YggS family pyridoxal phosphate-dependent enzyme -
  MMW58_RS04215 pilT 857752..858732 (+) 981 WP_159237127.1 type IV pilus twitching motility protein PilT Machinery gene
  MMW58_RS04220 - 859079..859732 (+) 654 WP_133600963.1 LuxR C-terminal-related transcriptional regulator -
  MMW58_RS04225 ruvX 859777..860193 (-) 417 WP_041147200.1 Holliday junction resolvase RuvX -
  MMW58_RS04230 - 860193..860756 (-) 564 WP_041147199.1 YqgE/AlgH family protein -
  MMW58_RS04235 gshB 861203..862150 (-) 948 WP_076946673.1 glutathione synthase -
  MMW58_RS04240 rsmE 862171..862902 (-) 732 WP_133600964.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  MMW58_RS04245 endA 862996..863703 (-) 708 WP_241722771.1 deoxyribonuclease I -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35206.38 Da        Isoelectric Point: 6.5831

>NTDB_id=662907 MMW58_RS04215 WP_159237127.1 857752..858732(+) (pilT) [Raoultella sp. HC6]
MELEEIVALSVKHNVSDLHLCNASAPRWRRQGRLEPAPFTSPEIGKILAQWLNDDQQAQWQASGQVDFALALPGGPRLRA
SAFAHTRGLSLALRLLPEACPRLDELCVPAAIAELLNEESGLILVTGATGSGKSTTLAAMVGYLNQRLDGHILTLEDPVE
FVHGSERCLVQQREIGRHAPSFAAALRVALRQDPDVILLGELRDSETIRLALTAAETGHLVMATLHTRGAAQAVERLVDV
FPAQEKEQVRGQLAGSLCAVLAQKLLPDISGGRVALFELLVNTPAVANLIREGKTHQLPGVMQTGQQSGMQTFTQSFQQR
VSAGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=662907 MMW58_RS04215 WP_159237127.1 857752..858732(+) (pilT) [Raoultella sp. HC6]
ATGGAGCTGGAAGAAATAGTGGCCCTTAGTGTAAAGCATAACGTCTCCGATCTACACCTGTGCAATGCCTCGGCGCCGCG
CTGGCGTCGTCAGGGGAGGCTGGAGCCCGCGCCCTTCACGTCGCCGGAGATCGGCAAAATACTCGCGCAGTGGCTCAATG
ACGATCAGCAGGCGCAGTGGCAGGCCAGCGGACAGGTTGATTTCGCGCTGGCGCTACCCGGCGGGCCGCGCCTTCGCGCC
AGCGCATTCGCCCACACGCGCGGGCTGTCGCTGGCGCTGCGCCTGCTGCCGGAAGCCTGCCCGCGGCTTGATGAGCTCTG
CGTTCCCGCCGCGATAGCCGAGCTGCTCAATGAAGAGAGCGGGCTGATCCTCGTGACCGGCGCCACCGGCAGCGGTAAAT
CGACCACCCTCGCGGCGATGGTCGGCTATCTCAATCAGCGCCTCGACGGCCATATCCTGACCCTTGAAGATCCGGTGGAG
TTTGTCCATGGCAGCGAGCGCTGCCTGGTCCAGCAGCGTGAAATTGGCCGCCACGCGCCGTCGTTTGCGGCGGCGCTGCG
GGTGGCGCTGCGCCAGGACCCGGACGTCATTCTGCTGGGCGAGCTGCGCGACAGCGAGACCATCCGCCTGGCGCTGACGG
CGGCGGAGACCGGGCACCTGGTGATGGCCACGCTGCATACCCGCGGCGCGGCGCAGGCGGTTGAGCGCCTGGTTGACGTT
TTTCCTGCCCAGGAAAAAGAGCAGGTTCGCGGCCAGCTGGCGGGAAGTTTATGCGCCGTACTGGCACAAAAATTATTACC
GGATATATCTGGCGGTAGAGTTGCGCTATTCGAGCTTTTGGTAAATACCCCGGCGGTGGCTAATTTGATCCGTGAGGGTA
AAACCCATCAGCTGCCCGGCGTTATGCAGACCGGACAGCAGAGCGGAATGCAGACTTTTACGCAAAGTTTTCAACAGCGT
GTCTCCGCAGGTCGCCTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(34-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Neisseria meningitidis 8013

50.305

100

0.506
  pilT Neisseria gonorrhoeae MS11

50

100

0.503
  pilT Vibrio cholerae strain A1552

49.847

100

0.5
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.847

100

0.5
  pilT Legionella pneumophila strain ERS1305867

46.789

100

0.469
  pilT Legionella pneumophila strain Lp02

46.789

100

0.469
  pilT Pseudomonas aeruginosa PAK

46.789

100

0.469
  pilT Pseudomonas stutzeri DSM 10701

46.177

100

0.463
  pilT Acinetobacter baylyi ADP1

45.872

100

0.46
  pilT Acinetobacter nosocomialis M2

45.566

100

0.457
  pilT Acinetobacter baumannii D1279779

45.26

100

0.454
  pilT Acinetobacter baumannii strain A118

45.26

100

0.454
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.849

97.239

0.426
  pilU Vibrio cholerae strain A1552

41.641

100

0.42
  pilU Pseudomonas stutzeri DSM 10701

40.303

100

0.408
  pilU Acinetobacter baylyi ADP1

37.578

98.773

0.371