Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   MN553_RS04055 Genome accession   NZ_CP093271
Coordinates   831209..832189 (+) Length   326 a.a.
NCBI ID   WP_004105710.1    Uniprot ID   A0A9P0U2X2
Organism   Klebsiella oxytoca strain C-21-18     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 826209..837189
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MN553_RS04025 (MN553_04025) - 826591..827577 (-) 987 WP_004115390.1 TRAP transporter substrate-binding protein -
  MN553_RS04030 (MN553_04030) hemW 827882..829018 (-) 1137 WP_024274715.1 radical SAM family heme chaperone HemW -
  MN553_RS04035 (MN553_04035) - 829011..829604 (-) 594 WP_023321631.1 XTP/dITP diphosphatase -
  MN553_RS04040 (MN553_04040) yggU 829617..829907 (-) 291 WP_004105716.1 DUF167 family protein YggU -
  MN553_RS04045 (MN553_04045) - 829904..830470 (-) 567 WP_004105714.1 YggT family protein -
  MN553_RS04050 (MN553_04050) - 830488..831192 (-) 705 WP_017145431.1 YggS family pyridoxal phosphate-dependent enzyme -
  MN553_RS04055 (MN553_04055) pilT 831209..832189 (+) 981 WP_004105710.1 type IV pilus twitching motility protein PilT Machinery gene
  MN553_RS04060 (MN553_04060) - 832538..833191 (+) 654 WP_004105708.1 LuxR C-terminal-related transcriptional regulator -
  MN553_RS04065 (MN553_04065) ruvX 833247..833663 (-) 417 WP_004105707.1 Holliday junction resolvase RuvX -
  MN553_RS04070 (MN553_04070) - 833663..834226 (-) 564 WP_004105705.1 YqgE/AlgH family protein -
  MN553_RS04075 (MN553_04075) gshB 834403..835350 (-) 948 WP_004105704.1 glutathione synthase -
  MN553_RS04080 (MN553_04080) rsmE 835371..836102 (-) 732 WP_004105702.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  MN553_RS04085 (MN553_04085) endA 836194..836901 (-) 708 WP_004105700.1 deoxyribonuclease I -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35124.23 Da        Isoelectric Point: 6.7168

>NTDB_id=662882 MN553_RS04055 WP_004105710.1 831209..832189(+) (pilT) [Klebsiella oxytoca strain C-21-18]
MELEEIVALSVKHNVSDLHLCNASAPRWRRQGKLEPAPFTSPDISKILHDWLNAEQLAQWQASGQVDFALAPAGGPRLRA
SAFAHSRGLSLALRLLAETCPQLEALGVPAALSELLNEESGLILVTGATGSGKSTTLAAMVGYLNQRLNGHILTLEDPVE
FIHHSERCLIQQREIGRHCPSFAAALRAALRQDPDVILLGELRDSETIRLALTAAETGHLVMATLHTRGAAQAVERLVDV
FPAQEKEQVRSQLAGSLCAVLAQKLFPDAAGGRVALYELLVNTPAVANLIREGKTHQLPGVMQTGQQSGMQTFTQSFQQR
TAAGLL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=662882 MN553_RS04055 WP_004105710.1 831209..832189(+) (pilT) [Klebsiella oxytoca strain C-21-18]
ATGGAACTGGAAGAAATAGTGGCCCTTAGTGTAAAGCATAACGTCTCCGATCTACACCTGTGCAATGCTTCAGCGCCGCG
CTGGCGTCGCCAGGGAAAGCTGGAGCCTGCGCCTTTTACCTCCCCTGATATCAGCAAAATACTCCATGACTGGCTCAACG
CCGAGCAGCTCGCGCAGTGGCAGGCCAGCGGGCAGGTCGATTTCGCTTTAGCGCCGGCTGGCGGGCCACGCCTTCGCGCC
AGCGCATTTGCCCACAGCCGCGGCCTGTCGCTGGCGCTGCGTCTGCTGGCGGAAACCTGCCCGCAGCTGGAGGCGCTCGG
TGTACCAGCCGCCTTAAGCGAGCTGCTCAATGAGGAGAGCGGGCTTATCCTCGTCACGGGCGCCACCGGCAGCGGGAAAT
CAACTACCCTGGCGGCGATGGTGGGCTATCTTAATCAGCGCCTCAATGGTCATATCCTGACGCTGGAAGATCCGGTCGAA
TTTATTCACCACAGCGAGCGTTGCCTGATCCAGCAGCGGGAAATCGGCCGCCACTGCCCCTCCTTTGCCGCGGCGTTACG
CGCTGCGCTGCGTCAGGATCCGGACGTCATTCTGTTAGGGGAACTGCGCGATAGCGAAACTATCCGCCTGGCACTTACGG
CAGCGGAGACCGGACATCTGGTTATGGCGACGCTGCATACGCGCGGCGCGGCGCAGGCGGTTGAAAGGCTGGTGGATGTT
TTTCCCGCCCAGGAGAAAGAGCAGGTTCGCAGCCAGCTGGCGGGAAGTTTGTGCGCGGTACTGGCACAAAAATTGTTTCC
GGATGCTGCAGGCGGCAGAGTTGCGCTATACGAGCTTTTAGTTAATACCCCAGCAGTGGCGAATTTGATCCGGGAGGGAA
AAACGCATCAGCTGCCCGGCGTGATGCAAACCGGGCAGCAATCTGGCATGCAAACATTTACGCAAAGCTTTCAGCAACGT
ACGGCAGCGGGTCTTTTATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.152

100

0.506
  pilT Vibrio cholerae strain A1552

50.152

100

0.506
  pilT Neisseria meningitidis 8013

48.182

100

0.488
  pilT Neisseria gonorrhoeae MS11

47.879

100

0.485
  pilT Legionella pneumophila strain Lp02

48.428

97.546

0.472
  pilT Legionella pneumophila strain ERS1305867

48.428

97.546

0.472
  pilT Pseudomonas stutzeri DSM 10701

45.897

100

0.463
  pilT Pseudomonas aeruginosa PAK

45.593

100

0.46
  pilT Acinetobacter baumannii strain A118

45.152

100

0.457
  pilT Acinetobacter baumannii D1279779

45.152

100

0.457
  pilT Acinetobacter baylyi ADP1

45.289

100

0.457
  pilT Acinetobacter nosocomialis M2

44.848

100

0.454
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.479

97.239

0.433
  pilU Pseudomonas stutzeri DSM 10701

41.463

100

0.417
  pilU Vibrio cholerae strain A1552

41.304

98.773

0.408
  pilU Acinetobacter baylyi ADP1

37.888

98.773

0.374