Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   PCAU_RS29005 Genome accession   NZ_AP014623
Coordinates   6374822..6375856 (-) Length   344 a.a.
NCBI ID   WP_096375700.1    Uniprot ID   -
Organism   Pseudomonas chlororaphis subsp. aurantiaca strain StFRB508     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6369822..6380856
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PCAU_RS28975 (PCAU_5739) ruvX 6369977..6370414 (+) 438 WP_009046019.1 Holliday junction resolvase RuvX -
  PCAU_RS28980 (PCAU_5740) pyrR 6370536..6371042 (+) 507 WP_007929976.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  PCAU_RS28985 (PCAU_5741) - 6371069..6372073 (+) 1005 WP_007929978.1 aspartate carbamoyltransferase catalytic subunit -
  PCAU_RS28990 (PCAU_5742) - 6372070..6373341 (+) 1272 WP_096375699.1 dihydroorotase -
  PCAU_RS28995 (PCAU_5743) - 6373436..6373855 (-) 420 WP_038575076.1 NINE protein -
  PCAU_RS29000 (PCAU_5744) - 6374106..6374738 (+) 633 WP_038575074.1 C40 family peptidase -
  PCAU_RS29005 (PCAU_5745) pilT 6374822..6375856 (-) 1035 WP_096375700.1 type IV pilus twitching motility protein PilT Machinery gene
  PCAU_RS29010 (PCAU_5746) - 6375914..6376612 (+) 699 WP_096375701.1 YggS family pyridoxal phosphate-dependent enzyme -
  PCAU_RS29015 (PCAU_5747) proC 6376647..6377465 (+) 819 WP_016704849.1 pyrroline-5-carboxylate reductase -
  PCAU_RS29020 (PCAU_5748) - 6377476..6378066 (+) 591 WP_009051315.1 YggT family protein -
  PCAU_RS29025 (PCAU_5749) - 6378080..6378373 (+) 294 WP_009046027.1 DUF167 domain-containing protein -
  PCAU_RS29030 (PCAU_5750) metX 6378581..6379720 (+) 1140 WP_009051317.1 homoserine O-succinyltransferase MetX -
  PCAU_RS29035 (PCAU_5751) metW 6379728..6380348 (+) 621 WP_023967992.1 methionine biosynthesis protein MetW -
  PCAU_RS29040 (PCAU_5752) - 6380376..6380810 (+) 435 WP_007921193.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37527.23 Da        Isoelectric Point: 7.1235

>NTDB_id=66280 PCAU_RS29005 WP_096375700.1 6374822..6375856(-) (pilT) [Pseudomonas chlororaphis subsp. aurantiaca strain StFRB508]
MDITELLAFSVKQGASDLHLSAGLPPMIRIDGDIRRINLPVLEAQQVHELILASMTDPQRAEFKAALEIDFSFAVPGLAR
FRVNAFNHQRGAGAVFRSIPAKVPSLDELGMGEVFRRISEVPRGLVLVTGPTGSGKSTTLAAMIDHLNQSRHQHILTIED
PIEFIHESKKCLINQREVHRDTRSFSTALRSALREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTVDRV
VDVFPAEEKAMVRSMLSESLQAVISQALVKRIGGGRVAAHEIMLGSAAIRNLIREDKVAQMYSAIQTGGAQGMQTLDMSL
KALVDAGLVSREHAREKARAPDSL

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=66280 PCAU_RS29005 WP_096375700.1 6374822..6375856(-) (pilT) [Pseudomonas chlororaphis subsp. aurantiaca strain StFRB508]
ATGGATATCACTGAGTTGCTGGCCTTCAGCGTCAAACAGGGCGCTTCGGACCTGCACCTCTCGGCCGGTTTGCCGCCGAT
GATTCGCATCGACGGCGATATTCGCCGGATCAACCTGCCGGTGCTGGAGGCGCAACAGGTGCACGAGCTGATCCTGGCCA
GCATGACGGACCCGCAGCGGGCCGAGTTCAAGGCCGCGCTGGAGATCGACTTTTCCTTCGCGGTACCGGGCCTGGCGCGC
TTTCGGGTCAATGCCTTCAACCATCAGCGCGGCGCTGGCGCGGTCTTTCGCAGCATTCCGGCCAAGGTGCCGAGCCTGGA
CGAACTGGGCATGGGCGAGGTGTTTCGCCGGATCAGCGAAGTGCCCCGCGGCCTGGTGCTGGTGACCGGGCCGACCGGTT
CGGGCAAGTCGACCACCCTGGCGGCGATGATCGATCACCTGAACCAGAGCCGGCACCAGCACATCCTCACCATCGAGGAC
CCCATCGAGTTCATCCACGAATCGAAGAAGTGCCTGATCAACCAGCGCGAGGTGCATCGCGACACCCGCAGCTTCTCCAC
GGCGCTGCGTTCGGCCCTGCGCGAAGACCCGGATGTGATCCTGGTCGGCGAGATGCGCGACCTGGAAACCATTCGCCTGG
CGCTGACCGCCGCGGAGACCGGGCATCTGGTGTTCGGCACCCTGCACACCACCTCGGCGGCGAAAACCGTGGACCGGGTG
GTGGACGTGTTTCCGGCCGAGGAGAAGGCGATGGTCCGCTCCATGCTTTCCGAATCGCTGCAGGCGGTGATTTCCCAGGC
ACTGGTGAAGCGTATCGGTGGAGGCCGGGTAGCGGCCCACGAGATCATGCTGGGCAGCGCGGCGATCCGTAACCTGATCC
GTGAGGACAAGGTGGCGCAGATGTACTCGGCGATCCAGACCGGCGGGGCGCAGGGGATGCAGACCCTGGACATGAGCCTG
AAGGCGCTGGTGGATGCGGGGCTGGTCAGTCGGGAGCACGCCCGGGAGAAGGCGCGGGCGCCGGACAGTCTTTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

81.105

100

0.811
  pilT Pseudomonas stutzeri DSM 10701

78.198

100

0.782
  pilT Acinetobacter nosocomialis M2

73.837

100

0.738
  pilT Acinetobacter baumannii D1279779

73.837

100

0.738
  pilT Acinetobacter baumannii strain A118

73.837

100

0.738
  pilT Acinetobacter baylyi ADP1

72.674

100

0.727
  pilT Legionella pneumophila strain Lp02

68.047

98.256

0.669
  pilT Legionella pneumophila strain ERS1305867

68.047

98.256

0.669
  pilT Neisseria meningitidis 8013

65.507

100

0.657
  pilT Neisseria gonorrhoeae MS11

65.217

100

0.654
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

66.172

97.965

0.648
  pilT Vibrio cholerae strain A1552

66.172

97.965

0.648
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.462

99.419

0.512
  pilU Vibrio cholerae strain A1552

40.58

100

0.407
  pilU Acinetobacter baylyi ADP1

40.462

100

0.407
  pilU Pseudomonas stutzeri DSM 10701

39.13

100

0.392

Multiple sequence alignment