Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   MKP10_RS21570 Genome accession   NZ_CP093164
Coordinates   4636344..4637378 (-) Length   344 a.a.
NCBI ID   WP_045423701.1    Uniprot ID   A0A365PUB1
Organism   Stutzerimonas zhaodongensis strain LQ01-5     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4631344..4642378
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MKP10_RS21550 (MKP10_21550) - 4632542..4632946 (-) 405 WP_045423709.1 NINE protein -
  MKP10_RS21555 (MKP10_21555) - 4633081..4634292 (+) 1212 WP_045423707.1 putative DNA modification/repair radical SAM protein -
  MKP10_RS21560 (MKP10_21560) - 4634292..4635122 (+) 831 WP_241529447.1 TIGR03915 family putative DNA repair protein -
  MKP10_RS21565 (MKP10_21565) pilU 4635123..4636268 (-) 1146 WP_045423703.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  MKP10_RS21570 (MKP10_21570) pilT 4636344..4637378 (-) 1035 WP_045423701.1 type IV pilus twitching motility protein PilT Machinery gene
  MKP10_RS21575 (MKP10_21575) - 4637445..4638158 (+) 714 WP_241529448.1 YggS family pyridoxal phosphate-dependent enzyme -
  MKP10_RS21580 (MKP10_21580) proC 4638172..4638999 (+) 828 WP_241529449.1 pyrroline-5-carboxylate reductase -
  MKP10_RS21585 (MKP10_21585) - 4639009..4639599 (+) 591 WP_045423695.1 YggT family protein -
  MKP10_RS21590 (MKP10_21590) - 4639721..4641682 (+) 1962 WP_241529450.1 dynamin-like GTPase family protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37905.62 Da        Isoelectric Point: 6.8360

>NTDB_id=662051 MKP10_RS21570 WP_045423701.1 4636344..4637378(-) (pilT) [Stutzerimonas zhaodongensis strain LQ01-5]
MDITELLAFSAKQGASDLHLSSGLPPMIRVDGDVRRINLPAMDHKQVHALIYDIMNDKQRKDFEEFLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLTMEDLGMGEVFRKITDVPRGLVLVTGPTGSGKSTTLAAMLDYLNSTKYHHILTIED
PIEFVHESKKCLVNQREVHRDTLGFSEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPAEEKSMVRSMLSESLQAVISQTLLKKVGGGRVAAHEIMIGTPAIRNLIREDKVAQMYSSIQTGGSLGMQTLDSCL
KGLLAKGLVSRDSAKEKAKQPENF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=662051 MKP10_RS21570 WP_045423701.1 4636344..4637378(-) (pilT) [Stutzerimonas zhaodongensis strain LQ01-5]
ATGGATATCACAGAGCTGCTGGCCTTCAGTGCCAAGCAGGGTGCGTCGGATTTGCACCTGTCTTCCGGCTTGCCGCCGAT
GATCCGCGTTGATGGCGACGTGCGCCGGATCAACCTCCCAGCGATGGACCACAAGCAGGTACACGCGCTGATCTACGACA
TCATGAACGACAAGCAGCGCAAGGATTTCGAAGAGTTCCTCGAGACCGATTTCTCGTTCGAAGTACCGGGCGTGGCGCGT
TTCCGGGTCAACGCCTTCAACCAGAACCGCGGCGCTGGTGCAGTATTCCGGACCATTCCGTCCAAGGTGCTGACCATGGA
AGACCTCGGCATGGGCGAGGTCTTCCGCAAGATTACCGACGTACCGCGCGGGCTGGTGCTGGTCACCGGGCCGACCGGTT
CGGGCAAGTCGACCACCCTGGCGGCGATGCTGGACTACCTGAACAGCACTAAGTACCACCACATCCTCACCATCGAGGAC
CCGATCGAATTCGTTCATGAATCGAAGAAGTGCCTGGTCAACCAGCGTGAAGTGCATCGAGACACGCTCGGCTTCTCCGA
AGCACTGCGTTCGGCGCTGCGTGAAGACCCGGACATCATTCTCGTCGGCGAAATGCGCGACTTGGAGACCATCCGCCTGG
CGCTGACTGCCGCGGAAACCGGCCACCTGGTGTTTGGCACCTTGCACACCACCTCGGCGGCCAAGACCATCGACCGGGTC
GTAGACGTGTTCCCGGCTGAAGAGAAGTCGATGGTCCGCTCGATGCTGTCCGAATCTCTGCAGGCTGTGATTTCCCAGAC
CCTGTTGAAAAAGGTCGGTGGCGGTCGGGTGGCGGCGCACGAGATCATGATCGGCACCCCTGCGATCCGTAACCTGATCC
GCGAGGACAAGGTGGCGCAGATGTACTCGTCCATCCAGACCGGCGGCTCGCTGGGCATGCAAACGCTCGATTCCTGCCTC
AAAGGGCTGCTCGCCAAGGGGCTGGTCTCGCGTGACAGTGCCAAAGAAAAAGCCAAACAGCCGGAAAACTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A365PUB1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

99.419

100

0.994
  pilT Pseudomonas aeruginosa PAK

93.605

100

0.936
  pilT Acinetobacter baumannii D1279779

83.721

100

0.837
  pilT Acinetobacter baumannii strain A118

83.721

100

0.837
  pilT Acinetobacter nosocomialis M2

83.43

100

0.834
  pilT Acinetobacter baylyi ADP1

82.849

100

0.828
  pilT Legionella pneumophila strain Lp02

74.26

98.256

0.73
  pilT Legionella pneumophila strain ERS1305867

74.26

98.256

0.73
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.139

97.965

0.677
  pilT Vibrio cholerae strain A1552

69.139

97.965

0.677
  pilT Neisseria meningitidis 8013

66.957

100

0.672
  pilT Neisseria gonorrhoeae MS11

66.667

100

0.669
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.174

100

0.523
  pilU Vibrio cholerae strain A1552

40.708

98.547

0.401
  pilU Pseudomonas stutzeri DSM 10701

40.597

97.384

0.395
  pilU Acinetobacter baylyi ADP1

38.728

100

0.39