Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   MND42_RS03995 Genome accession   NZ_CP093154
Coordinates   818234..819214 (+) Length   326 a.a.
NCBI ID   WP_023309128.1    Uniprot ID   A0AAQ0Y050
Organism   Enterobacter asburiae strain 5549     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 813234..824214
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MND42_RS03955 (MND42_03955) - 813318..813644 (+) 327 WP_014885198.1 YggL family protein -
  MND42_RS03960 (MND42_03960) - 813701..814417 (+) 717 WP_023309135.1 DUF2884 domain-containing protein -
  MND42_RS03965 (MND42_03965) - 814500..814838 (-) 339 WP_045403926.1 endonuclease domain-containing protein -
  MND42_RS03970 (MND42_03970) hemW 814902..816044 (-) 1143 WP_241510649.1 radical SAM family heme chaperone HemW -
  MND42_RS03975 (MND42_03975) - 816037..816630 (-) 594 WP_045403921.1 XTP/dITP diphosphatase -
  MND42_RS03980 (MND42_03980) yggU 816634..816930 (-) 297 WP_023333349.1 DUF167 family protein YggU -
  MND42_RS03985 (MND42_03985) - 816927..817493 (-) 567 WP_023309130.1 YggT family protein -
  MND42_RS03990 (MND42_03990) - 817515..818216 (-) 702 WP_045403918.1 YggS family pyridoxal phosphate-dependent enzyme -
  MND42_RS03995 (MND42_03995) pilT 818234..819214 (+) 981 WP_023309128.1 type IV pilus twitching motility protein PilT Machinery gene
  MND42_RS04000 (MND42_04000) ruvX 819225..819641 (-) 417 WP_021242076.1 Holliday junction resolvase RuvX -
  MND42_RS04005 (MND42_04005) - 819641..820201 (-) 561 WP_028014368.1 YqgE/AlgH family protein -
  MND42_RS04010 (MND42_04010) gshB 820289..821236 (-) 948 WP_029739222.1 glutathione synthase -
  MND42_RS04015 (MND42_04015) rsmE 821256..821987 (-) 732 WP_045403915.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  MND42_RS04020 (MND42_04020) endA 822039..822746 (-) 708 WP_023333343.1 deoxyribonuclease I -
  MND42_RS04025 (MND42_04025) - 822841..823338 (-) 498 WP_032667932.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35521.53 Da        Isoelectric Point: 6.9108

>NTDB_id=662004 MND42_RS03995 WP_023309128.1 818234..819214(+) (pilT) [Enterobacter asburiae strain 5549]
MDVEEIVALSVKHNVSDLHLCSDSPPRWRRLGRLEPAPFPPPDVGALLKAWLNDEQQGIWWAKGQVDFASTVTGGQRLRG
SAFKQMRGVSVTLRLLPRSCPQLSSLGAPRAIPELLSNDAGLILVTGATGSGKSTTLAAMVDFLNHHADGHILTLEDPVE
FIYQSERCLIQQREIGQHSPSFAEALRSALRQDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGASQAIERLVDT
FPAQEKDPVRNQLGGSLRAVLAQRLLPDLQGGRVALYELLVNTAAAANLIREGKTWQLPGIIQTGQQAGMQNFDQSLAER
RAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=662004 MND42_RS03995 WP_023309128.1 818234..819214(+) (pilT) [Enterobacter asburiae strain 5549]
ATGGATGTGGAAGAAATTGTGGCTCTTAGTGTAAAGCATAACGTCTCCGATCTACACCTGTGCAGTGATTCGCCACCGCG
CTGGCGCAGATTAGGTCGGCTTGAACCCGCTCCTTTTCCGCCTCCCGATGTCGGAGCGTTATTGAAAGCGTGGCTCAACG
ATGAGCAGCAGGGGATATGGTGGGCAAAGGGGCAGGTGGATTTTGCCTCGACGGTGACGGGAGGCCAGCGGCTGCGCGGC
AGTGCCTTTAAGCAGATGAGAGGTGTCTCTGTGACGCTGCGGCTGCTGCCGCGTAGCTGCCCACAGCTCTCTTCGCTGGG
CGCGCCGCGGGCGATCCCGGAACTGTTGTCCAATGACGCCGGGCTGATTCTGGTCACGGGGGCGACCGGCAGCGGCAAAT
CCACTACGCTGGCGGCGATGGTCGATTTTCTCAACCACCATGCTGACGGCCATATCCTCACGCTTGAAGATCCGGTGGAG
TTTATCTACCAGAGCGAACGTTGTCTGATCCAGCAGCGGGAGATAGGCCAGCACAGCCCGTCATTTGCTGAAGCGCTGCG
CAGCGCCTTACGCCAGGATCCGGACGTTATTCTGCTGGGGGAGCTGCGCGACAGCGAAACGATCCGCCTGGCGCTGACGG
CGGCGGAAACCGGACACCTGGTGCTGGCGACGCTGCATACGCGCGGGGCATCGCAGGCGATTGAACGGCTGGTCGATACG
TTCCCGGCGCAGGAGAAAGATCCGGTGCGTAACCAGCTGGGCGGCAGCCTGCGAGCGGTGCTGGCGCAGAGGCTGCTTCC
CGATCTGCAGGGCGGGCGCGTCGCGTTGTATGAACTGCTGGTGAACACTGCGGCGGCGGCGAATTTGATTCGTGAAGGGA
AAACGTGGCAACTGCCCGGGATCATTCAAACCGGTCAGCAGGCAGGAATGCAGAACTTTGACCAGAGCCTGGCGGAGAGA
CGGGCGCAGGGCCGGCTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(74-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.459

100

0.506
  pilT Vibrio cholerae strain A1552

50.459

100

0.506
  pilT Pseudomonas stutzeri DSM 10701

48.624

100

0.488
  pilT Legionella pneumophila strain Lp02

49.379

98.773

0.488
  pilT Legionella pneumophila strain ERS1305867

49.379

98.773

0.488
  pilT Acinetobacter baylyi ADP1

48.318

100

0.485
  pilT Acinetobacter baumannii D1279779

48.318

100

0.485
  pilT Acinetobacter baumannii strain A118

48.318

100

0.485
  pilT Neisseria meningitidis 8013

47.866

100

0.482
  pilT Neisseria gonorrhoeae MS11

47.561

100

0.479
  pilT Acinetobacter nosocomialis M2

48.75

98.16

0.479
  pilT Pseudomonas aeruginosa PAK

47.706

100

0.479
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.478

98.773

0.429
  pilU Pseudomonas stutzeri DSM 10701

39.394

100

0.399
  pilU Vibrio cholerae strain A1552

39.441

98.773

0.39
  pilU Acinetobacter baylyi ADP1

36.646

98.773

0.362