Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   MME54_RS02045 Genome accession   NZ_CP092918
Coordinates   454180..455214 (+) Length   344 a.a.
NCBI ID   WP_054986071.1    Uniprot ID   A0A0N8RNH7
Organism   Pseudomonas syringae pv. helianthi strain LMG 5067     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 449180..460214
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MME54_RS02015 (MME54_02015) rdgB 449258..449851 (-) 594 WP_054986066.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -
  MME54_RS02020 (MME54_02020) metW 449885..450505 (-) 621 WP_003414343.1 methionine biosynthesis protein MetW -
  MME54_RS02025 (MME54_02025) metX 450513..451652 (-) 1140 WP_054986067.1 homoserine O-succinyltransferase MetX -
  MME54_RS02030 (MME54_02030) - 451981..452571 (-) 591 WP_054986068.1 YggT family protein -
  MME54_RS02035 (MME54_02035) proC 452597..453415 (-) 819 WP_054986069.1 pyrroline-5-carboxylate reductase -
  MME54_RS02040 (MME54_02040) - 453438..454124 (-) 687 WP_054986070.1 YggS family pyridoxal phosphate-dependent enzyme -
  MME54_RS02045 (MME54_02045) pilT 454180..455214 (+) 1035 WP_054986071.1 type IV pilus twitching motility protein PilT Machinery gene
  MME54_RS02050 (MME54_02050) - 455327..456004 (-) 678 WP_054986072.1 C40 family peptidase -
  MME54_RS02055 (MME54_02055) - 456255..456659 (+) 405 WP_054986073.1 NINE protein -
  MME54_RS02060 (MME54_02060) - 456975..458246 (-) 1272 WP_054986074.1 dihydroorotase -
  MME54_RS02065 (MME54_02065) - 458243..459247 (-) 1005 WP_054986075.1 aspartate carbamoyltransferase catalytic subunit -
  MME54_RS02070 (MME54_02070) pyrR 459260..459772 (-) 513 WP_054986076.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37922.74 Da        Isoelectric Point: 7.0226

>NTDB_id=661116 MME54_RS02045 WP_054986071.1 454180..455214(+) (pilT) [Pseudomonas syringae pv. helianthi strain LMG 5067]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPPLDAKEVKALIYDIMNDKQRQDFEERLETDFSFEVPGVAR
FRVNAFNQNRGSGAVFRTIPSKILSMEDLGMGSVFRKITDVARGLILVTGPTGSGKSTTLAAMIDYLNCNKHHHILTIED
PIEFVHESKKCLVNQREVHRDTLGFSEALRSALREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRI
VDVFPAQEKSMIRSMLSESLHAVVSQALLKKVGGGRVAAHEIMMGTPAIRNLIREDKVAQMYSSIQTGGSVGMQTLDMCL
ADLVKKGLITRESARERAKVPDNF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=661116 MME54_RS02045 WP_054986071.1 454180..455214(+) (pilT) [Pseudomonas syringae pv. helianthi strain LMG 5067]
ATGGATATTACCGAGCTGCTGGCCTTCAGTGCCAAACAGGGCGCGTCGGACTTGCACCTCTCTGCAGGTCTGCCACCGAT
GATCCGCGTTGACGGCGATGTGCGGCGTATCAACCTGCCCCCGCTGGACGCCAAGGAGGTCAAGGCGCTGATCTACGACA
TCATGAACGACAAGCAGCGCCAGGATTTCGAAGAGCGCCTGGAGACCGACTTTTCGTTTGAAGTACCGGGCGTTGCGCGC
TTTCGGGTCAACGCATTCAACCAGAATCGCGGTTCAGGCGCGGTATTCCGGACCATCCCCTCGAAAATCCTGAGCATGGA
AGACCTGGGGATGGGCAGTGTGTTTCGCAAGATTACCGACGTGGCGCGTGGCCTGATTCTGGTCACCGGGCCAACCGGAT
CAGGCAAGTCGACGACCCTGGCGGCAATGATCGACTACCTCAACTGCAACAAGCACCACCACATCCTGACCATTGAGGAC
CCGATCGAATTCGTCCACGAGTCGAAGAAGTGCCTGGTCAACCAGCGTGAGGTTCACCGCGATACGCTGGGCTTCTCGGA
GGCACTGCGTTCGGCCTTGCGCGAAGATCCGGATGTGATTCTGGTCGGTGAAATGCGCGACCTGGAGACCATCCGCCTGG
CGCTGACCGCTGCGGAAACCGGCCATCTGGTGTTCGGCACGCTGCACACCACATCGGCGGCCAAGACCATCGACCGGATC
GTGGATGTATTTCCGGCCCAGGAAAAATCGATGATTCGCTCAATGCTCTCCGAATCGCTGCACGCAGTCGTCTCCCAGGC
GCTGCTCAAGAAGGTTGGCGGCGGACGGGTAGCAGCGCATGAAATCATGATGGGCACCCCAGCGATCCGTAACCTGATTC
GCGAGGACAAGGTGGCACAGATGTATTCATCGATCCAGACCGGTGGTTCGGTGGGTATGCAGACGCTGGATATGTGTCTG
GCGGATCTGGTGAAGAAGGGCCTGATCACCCGCGAAAGCGCCAGGGAACGGGCCAAGGTGCCGGACAATTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0N8RNH7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

88.953

100

0.89
  pilT Pseudomonas aeruginosa PAK

87.5

100

0.875
  pilT Acinetobacter nosocomialis M2

78.488

100

0.785
  pilT Acinetobacter baumannii D1279779

78.488

100

0.785
  pilT Acinetobacter baumannii strain A118

78.488

100

0.785
  pilT Acinetobacter baylyi ADP1

77.907

100

0.779
  pilT Legionella pneumophila strain Lp02

73.964

98.256

0.727
  pilT Legionella pneumophila strain ERS1305867

73.964

98.256

0.727
  pilT Neisseria meningitidis 8013

68.235

98.837

0.674
  pilT Neisseria gonorrhoeae MS11

67.941

98.837

0.672
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

66.766

97.965

0.654
  pilT Vibrio cholerae strain A1552

66.766

97.965

0.654
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.333

100

0.535
  pilU Acinetobacter baylyi ADP1

41.04

100

0.413
  pilU Pseudomonas stutzeri DSM 10701

41.791

97.384

0.407
  pilU Vibrio cholerae strain A1552

40.597

97.384

0.395