Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   MJ613_RS03870 Genome accession   NZ_CP092860
Coordinates   799070..800050 (+) Length   326 a.a.
NCBI ID   WP_278085745.1    Uniprot ID   -
Organism   Citrobacter braakii strain P1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 794070..805050
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MJ613_RS03840 (MJ613_03840) ansB 794442..795488 (+) 1047 WP_016154404.1 L-asparaginase 2 -
  MJ613_RS03845 (MJ613_03845) hemW 795746..796882 (-) 1137 WP_185654438.1 radical SAM family heme chaperone HemW -
  MJ613_RS03850 (MJ613_03850) - 796875..797468 (-) 594 WP_185654439.1 XTP/dITP diphosphatase -
  MJ613_RS03855 (MJ613_03855) yggU 797476..797766 (-) 291 WP_005123286.1 DUF167 family protein YggU -
  MJ613_RS03860 (MJ613_03860) - 797763..798329 (-) 567 WP_016154401.1 YggT family protein -
  MJ613_RS03865 (MJ613_03865) - 798348..799052 (-) 705 WP_101700213.1 YggS family pyridoxal phosphate-dependent enzyme -
  MJ613_RS03870 (MJ613_03870) pilT 799070..800050 (+) 981 WP_278085745.1 type IV pilus twitching motility protein PilT Machinery gene
  MJ613_RS03875 (MJ613_03875) ruvX 800056..800463 (-) 408 WP_185654441.1 Holliday junction resolvase RuvX -
  MJ613_RS03880 (MJ613_03880) - 800463..801026 (-) 564 WP_170975110.1 YqgE/AlgH family protein -
  MJ613_RS03885 (MJ613_03885) gshB 801203..802150 (-) 948 WP_016154394.1 glutathione synthase -
  MJ613_RS03890 (MJ613_03890) rsmE 802163..802894 (-) 732 WP_016154393.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  MJ613_RS03895 (MJ613_03895) endA 802970..803677 (-) 708 WP_016154392.1 deoxyribonuclease I -
  MJ613_RS03900 (MJ613_03900) - 803772..804269 (-) 498 WP_016157438.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35876.99 Da        Isoelectric Point: 6.0393

>NTDB_id=660855 MJ613_RS03870 WP_278085745.1 799070..800050(+) (pilT) [Citrobacter braakii strain P1]
MNMEEIVALSVKHNVSDLHLCNAWPARWRIGGKVEIAPFTPPDVENLLTCWLSEQQQVQWREQGQIDFALTLADSRRLRA
SAFAHQQGTSLALRLLPLECPRLDDLQTPEAIPELLHGENGLVLVTGATGSGKSTTLAAMVEYLNQHIAGYILTLEDPIE
YRYTSLRCLIQQREVGVHCASFAAGLRGALREDPDVILLGELRDVETIRLALTAAETGHLVLATLHTRGAAQAIARLVDT
FPAQEKDPVRNQLADSLRAVLSQKLEEDKQGGRVALFELLVNTPAVGNLIREGKTHQLPGVIQTGQQTGMQTFAQSLQQR
QAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=660855 MJ613_RS03870 WP_278085745.1 799070..800050(+) (pilT) [Citrobacter braakii strain P1]
ATGAATATGGAAGAAATAGTGGCCCTTAGTGTAAAGCATAACGTGTCGGATCTACACCTGTGCAATGCATGGCCTGCGCG
CTGGCGCATAGGTGGAAAAGTCGAAATAGCACCATTTACTCCCCCTGACGTGGAGAATCTGCTGACGTGCTGGCTCAGTG
AGCAACAACAGGTACAGTGGCGGGAACAGGGGCAGATTGATTTTGCCCTTACGCTGGCGGACTCCCGGCGTCTACGCGCC
AGCGCATTTGCTCATCAGCAGGGAACCTCGCTGGCGCTAAGATTGCTACCGCTTGAATGTCCTCGTTTAGACGATCTTCA
GACCCCGGAGGCCATACCTGAACTGCTGCACGGTGAAAATGGATTAGTTCTGGTCACTGGAGCCACCGGCAGCGGTAAAT
CAACCACCCTGGCGGCGATGGTGGAGTACCTTAATCAACATATTGCGGGGTATATTCTGACGCTGGAAGATCCGATTGAA
TATCGCTACACCAGCCTGCGTTGTCTTATTCAACAGCGGGAGGTGGGCGTACACTGCGCTTCTTTTGCTGCCGGTTTGCG
CGGCGCGTTACGCGAAGATCCCGACGTCATTTTGCTGGGCGAGCTGCGCGACGTGGAAACTATTCGTTTGGCCTTAACGG
CAGCGGAGACCGGGCATCTGGTGTTGGCAACCTTACATACGCGAGGGGCGGCGCAGGCCATCGCGCGGCTGGTGGACACC
TTTCCCGCTCAGGAGAAAGATCCGGTGCGTAACCAGCTGGCAGACAGCCTGCGGGCGGTACTTTCGCAAAAGCTGGAGGA
AGATAAGCAGGGAGGACGCGTGGCGCTATTCGAGCTGCTCGTCAACACGCCCGCCGTGGGTAATTTGATCCGCGAAGGGA
AAACGCATCAGCTACCCGGAGTGATTCAAACCGGGCAGCAGACAGGTATGCAGACGTTTGCACAAAGTCTGCAGCAGCGC
CAGGCGCAGGGGCGACTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(56-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

50.459

100

0.506
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.459

100

0.506
  pilT Neisseria meningitidis 8013

46.646

100

0.469
  pilT Neisseria gonorrhoeae MS11

46.341

100

0.466
  pilT Acinetobacter baylyi ADP1

46.177

100

0.463
  pilT Legionella pneumophila strain ERS1305867

46.894

98.773

0.463
  pilT Legionella pneumophila strain Lp02

46.894

98.773

0.463
  pilT Acinetobacter baumannii D1279779

45.872

100

0.46
  pilT Pseudomonas stutzeri DSM 10701

45.872

100

0.46
  pilT Acinetobacter baumannii strain A118

45.872

100

0.46
  pilT Acinetobacter nosocomialis M2

45.566

100

0.457
  pilT Pseudomonas aeruginosa PAK

45.566

100

0.457
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.956

97.239

0.408
  pilU Pseudomonas stutzeri DSM 10701

38.485

100

0.39
  pilU Vibrio cholerae strain A1552

40.323

95.092

0.383
  pilU Acinetobacter baylyi ADP1

36.533

99.08

0.362