Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   MLC69_RS28095 Genome accession   NZ_CP092830
Coordinates   6143305..6144339 (-) Length   344 a.a.
NCBI ID   WP_078050559.1    Uniprot ID   -
Organism   Pseudomonas azotoformans strain ZY-1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6138305..6149339
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MLC69_RS28065 (MLC69_28065) ruvX 6138384..6138821 (+) 438 WP_005792259.1 Holliday junction resolvase RuvX -
  MLC69_RS28070 (MLC69_28070) pyrR 6138894..6139400 (+) 507 WP_078050555.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  MLC69_RS28075 (MLC69_28075) - 6139425..6140429 (+) 1005 WP_078050556.1 aspartate carbamoyltransferase catalytic subunit -
  MLC69_RS28080 (MLC69_28080) - 6140426..6141697 (+) 1272 WP_078050557.1 dihydroorotase -
  MLC69_RS28085 (MLC69_28085) - 6141847..6142257 (-) 411 WP_178076816.1 TM2 domain-containing protein -
  MLC69_RS28090 (MLC69_28090) - 6142526..6143164 (+) 639 WP_033897060.1 C40 family peptidase -
  MLC69_RS28095 (MLC69_28095) pilT 6143305..6144339 (-) 1035 WP_078050559.1 type IV pilus twitching motility protein PilT Machinery gene
  MLC69_RS28100 (MLC69_28100) - 6144397..6145083 (+) 687 WP_025859577.1 YggS family pyridoxal phosphate-dependent enzyme -
  MLC69_RS28105 (MLC69_28105) proC 6145117..6145935 (+) 819 WP_078050560.1 pyrroline-5-carboxylate reductase -
  MLC69_RS28110 (MLC69_28110) - 6145945..6146535 (+) 591 WP_058426554.1 YggT family protein -
  MLC69_RS28115 (MLC69_28115) - 6146743..6147882 (+) 1140 WP_078050561.1 homoserine O-acetyltransferase -
  MLC69_RS28120 (MLC69_28120) metW 6147890..6148510 (+) 621 WP_078050562.1 methionine biosynthesis protein MetW -
  MLC69_RS28125 (MLC69_28125) - 6148532..6148966 (+) 435 WP_078050563.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37661.39 Da        Isoelectric Point: 6.9910

>NTDB_id=660645 MLC69_RS28095 WP_078050559.1 6143305..6144339(-) (pilT) [Pseudomonas azotoformans strain ZY-1]
MDITELLTASVRRGASDLHLSAGLAPMLRVDGEVWPLDWPVLSPSQVADLLSPLLNQYQQKDFETSLETDFAFELPGVAR
FRANVFQQNRGMGAVFRTIPSEVRSLESLGLGEVFRRIAQLPRGLVLVTGPTGSGKSTTLAAMIDYLNQHRRQHILTLED
PIEFIHTPKTALINQRQVHRDTHDFSTALRSALREDPDVILVGELRDLDTIRLALTAAETGHLVFGTLHTSSAAKTVDRL
VDVFPAGEKAMVRSMLSESLQAVVSQVLVKKIGGGRVAAHEIMLGTPAIRNLIREDKVAQMVSAIQTGGALGMKTLDMSL
KRLVGEGLISREEAREKARVPADL

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=660645 MLC69_RS28095 WP_078050559.1 6143305..6144339(-) (pilT) [Pseudomonas azotoformans strain ZY-1]
ATGGATATCACTGAATTACTCACGGCCAGTGTGCGCCGTGGCGCTTCCGACCTGCACTTGTCGGCCGGCCTGGCGCCGAT
GCTGCGGGTGGATGGCGAGGTCTGGCCGCTGGATTGGCCGGTGCTTTCACCGTCGCAAGTCGCGGACTTATTGAGCCCGT
TGCTCAATCAGTACCAACAAAAAGATTTCGAAACATCTCTAGAAACAGATTTCGCCTTCGAACTGCCCGGCGTAGCGCGA
TTCCGGGCCAACGTGTTCCAACAGAATCGCGGCATGGGCGCGGTATTTCGCACCATCCCGTCCGAGGTCCGGAGCCTGGA
GAGCCTTGGCCTTGGCGAGGTGTTCCGGCGTATCGCCCAATTGCCACGGGGCTTGGTACTGGTTACCGGCCCGACCGGCT
CGGGCAAGTCCACCACCCTGGCGGCGATGATCGATTATCTCAATCAGCATCGGCGCCAGCACATCCTCACCCTCGAAGAC
CCCATCGAATTTATCCACACCCCCAAGACCGCCCTGATCAACCAGCGCCAGGTGCACCGGGACACCCATGACTTTTCCAC
CGCCCTGCGCTCCGCCCTTCGGGAAGACCCTGATGTGATCCTGGTGGGCGAACTGCGCGACCTGGACACCATTCGCCTCG
CGCTGACGGCGGCTGAAACCGGGCACCTGGTGTTTGGCACCCTGCACACCAGTTCAGCGGCAAAGACCGTAGACCGGCTG
GTGGACGTGTTTCCGGCTGGGGAAAAGGCCATGGTCCGCTCGATGTTGTCGGAGTCGTTGCAGGCGGTGGTGTCCCAAGT
GCTGGTAAAGAAGATCGGCGGCGGACGCGTGGCGGCCCATGAAATCATGCTGGGCACGCCGGCCATTCGGAACTTGATCC
GGGAGGACAAGGTGGCGCAGATGGTTTCAGCGATACAGACGGGTGGGGCGTTGGGGATGAAGACGCTGGATATGAGTTTG
AAGAGGTTGGTCGGAGAGGGGTTGATCAGCCGGGAAGAGGCGCGGGAGAAGGCGAGGGTGCCAGCAGACCTATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

74.419

100

0.744
  pilT Pseudomonas stutzeri DSM 10701

72.384

100

0.724
  pilT Acinetobacter nosocomialis M2

70.381

99.128

0.698
  pilT Acinetobacter baumannii D1279779

70.381

99.128

0.698
  pilT Acinetobacter baumannii strain A118

70.381

99.128

0.698
  pilT Acinetobacter baylyi ADP1

70.381

99.128

0.698
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

64.688

97.965

0.634
  pilT Vibrio cholerae strain A1552

64.688

97.965

0.634
  pilT Legionella pneumophila strain Lp02

64.201

98.256

0.631
  pilT Legionella pneumophila strain ERS1305867

64.201

98.256

0.631
  pilT Neisseria meningitidis 8013

61.765

98.837

0.61
  pilT Neisseria gonorrhoeae MS11

61.471

98.837

0.608
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.565

100

0.497
  pilU Pseudomonas stutzeri DSM 10701

39.13

100

0.392
  pilU Vibrio cholerae strain A1552

38.841

100

0.39
  pilU Acinetobacter baylyi ADP1

38.439

100

0.387