Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   AVAEIV_RS01505 Genome accession   NZ_CP092752
Coordinates   288842..289876 (+) Length   344 a.a.
NCBI ID   WP_302227938.1    Uniprot ID   -
Organism   Azotobacter vinelandii strain AEIV     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 283842..294876
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AVAEIV_RS01470 (AVAEIV_000291) - 283971..284390 (-) 420 WP_061288208.1 DUF4426 domain-containing protein -
  AVAEIV_RS01475 (AVAEIV_000292) metW 284452..285045 (-) 594 WP_041807492.1 methionine biosynthesis protein MetW -
  AVAEIV_RS01480 (AVAEIV_000293) metX 285053..286144 (-) 1092 WP_050764324.1 homoserine O-succinyltransferase MetX -
  AVAEIV_RS01485 (AVAEIV_000294) - 286321..286620 (-) 300 WP_012698993.1 DUF167 domain-containing protein -
  AVAEIV_RS01490 (AVAEIV_000295) - 286620..287213 (-) 594 WP_061288210.1 YggT family protein -
  AVAEIV_RS01495 (AVAEIV_000296) proC 287228..288046 (-) 819 WP_302227937.1 pyrroline-5-carboxylate reductase -
  AVAEIV_RS01500 (AVAEIV_000297) - 288065..288769 (-) 705 WP_012698996.1 YggS family pyridoxal phosphate-dependent enzyme -
  AVAEIV_RS01505 (AVAEIV_000298) pilT 288842..289876 (+) 1035 WP_302227938.1 type IV pilus twitching motility protein PilT Machinery gene
  AVAEIV_RS01510 (AVAEIV_000300) - 290477..290878 (+) 402 WP_012698998.1 NINE protein -
  AVAEIV_RS01515 (AVAEIV_000301) - 290939..292210 (-) 1272 WP_012698999.1 dihydroorotase -
  AVAEIV_RS01520 (AVAEIV_000302) - 292210..293220 (-) 1011 WP_012699000.1 aspartate carbamoyltransferase catalytic subunit -
  AVAEIV_RS01525 (AVAEIV_000303) pyrR 293503..294006 (-) 504 WP_012699001.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  AVAEIV_RS01530 (AVAEIV_000304) ruvX 294050..294478 (-) 429 WP_012699002.1 Holliday junction resolvase RuvX -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37888.59 Da        Isoelectric Point: 6.8403

>NTDB_id=660063 AVAEIV_RS01505 WP_302227938.1 288842..289876(+) (pilT) [Azotobacter vinelandii strain AEIV]
MDITELLAFGARQGASDLHLSAGLPPMIRVDGDVRRIDLPPLDHKQVHALIHDIMNDKQRKDYETFLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSRVPSMDELEMGEVFRKIAEVPRGLVLVTGATGSGKSTTLAAMLDYLNGSRHQHILTIED
PIEFVHEPKKCLINQREVHRDTLGFAEALRSALREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPAEEKAMVRSMLSESLQAVISQTLLKRVGGGRVAAREIMIGTPAIRNLIREDKVAQMYSAIQTGGSLGMQTLDMAL
KSLLSRGAISRESAREKAKIPDSF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=660063 AVAEIV_RS01505 WP_302227938.1 288842..289876(+) (pilT) [Azotobacter vinelandii strain AEIV]
ATGGACATCACCGAACTGCTCGCCTTCGGCGCCAGGCAGGGCGCGTCGGACCTGCACCTTTCCGCCGGCCTGCCGCCGAT
GATCCGCGTCGACGGCGACGTGCGCCGCATCGACCTGCCGCCGCTGGACCACAAGCAGGTGCACGCACTGATCCACGACA
TCATGAACGACAAGCAGCGCAAGGATTACGAAACGTTTCTCGAAACCGACTTCTCCTTCGAGGTGCCGGGCGTGGCGCGC
TTCCGGGTCAACGCCTTCAACCAGAACCGTGGCGCCGGCGCGGTGTTCCGCACCATTCCGTCGCGCGTCCCGAGCATGGA
CGAGCTGGAGATGGGCGAGGTGTTCAGGAAGATCGCCGAGGTGCCGCGCGGCCTGGTGCTGGTCACCGGCGCCACTGGCT
CGGGCAAGTCGACCACCCTGGCGGCCATGCTCGACTACCTGAACGGCAGCAGGCACCAGCACATCCTCACCATCGAGGAC
CCGATCGAATTCGTCCACGAGCCGAAGAAGTGCCTGATCAACCAGCGCGAAGTGCACCGCGACACCCTGGGCTTCGCCGA
GGCGCTGCGTTCGGCGCTGCGCGAGGACCCGGACGTCATCCTGGTCGGCGAGATGCGCGACCTGGAAACCATCCGCCTGG
CGCTGACCGCCGCGGAAACCGGCCACCTGGTGTTCGGCACCCTGCACACCACCTCGGCGGCCAAGACCATCGACCGGGTG
GTCGACGTGTTTCCCGCGGAGGAGAAGGCGATGGTCCGCTCGATGCTCTCCGAATCACTGCAGGCGGTGATCTCCCAGAC
CCTGCTGAAGAGGGTCGGCGGCGGCCGGGTGGCGGCCCGCGAGATCATGATCGGCACCCCGGCGATCCGCAATCTGATCC
GCGAGGACAAGGTGGCGCAGATGTACTCGGCGATCCAGACCGGCGGCTCGCTGGGCATGCAGACCCTGGACATGGCGTTG
AAGAGCCTGCTGTCCAGGGGCGCGATCAGCCGCGAGAGCGCCCGCGAGAAGGCGAAGATTCCCGACAGTTTCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

88.081

100

0.881
  pilT Pseudomonas stutzeri DSM 10701

87.791

100

0.878
  pilT Acinetobacter nosocomialis M2

80.233

100

0.802
  pilT Acinetobacter baumannii D1279779

79.942

100

0.799
  pilT Acinetobacter baumannii strain A118

79.942

100

0.799
  pilT Acinetobacter baylyi ADP1

79.651

100

0.797
  pilT Legionella pneumophila strain Lp02

71.302

98.256

0.701
  pilT Legionella pneumophila strain ERS1305867

71.302

98.256

0.701
  pilT Neisseria meningitidis 8013

67.536

100

0.677
  pilT Neisseria gonorrhoeae MS11

67.246

100

0.674
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.941

98.837

0.672
  pilT Vibrio cholerae strain A1552

67.941

98.837

0.672
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.884

100

0.52
  pilU Vibrio cholerae strain A1552

41.888

98.547

0.413
  pilU Acinetobacter baylyi ADP1

40.173

100

0.404
  pilU Pseudomonas stutzeri DSM 10701

41.194

97.384

0.401