Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   AVFI_RS02250 Genome accession   NZ_CP092712
Coordinates   470687..471724 (+) Length   345 a.a.
NCBI ID   WP_054775636.1    Uniprot ID   -
Organism   Aliivibrio fischeri ATCC 7744 = JCM 18803 = DSM 507     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 465687..476724
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AVFI_RS02215 (AVFI_02215) hemW 465882..467042 (-) 1161 WP_012533273.1 radical SAM family heme chaperone HemW -
  AVFI_RS02220 (AVFI_02220) - 467046..467642 (-) 597 WP_011261211.1 XTP/dITP diphosphatase -
  AVFI_RS02225 (AVFI_02225) - 467653..468084 (-) 432 WP_054775633.1 DUF4426 domain-containing protein -
  AVFI_RS02230 (AVFI_02230) yggU 468154..468441 (-) 288 WP_012533617.1 DUF167 family protein YggU -
  AVFI_RS02235 (AVFI_02235) - 468449..469003 (-) 555 WP_012533297.1 YggT family protein -
  AVFI_RS02240 (AVFI_02240) proC 469052..469870 (-) 819 WP_054775634.1 pyrroline-5-carboxylate reductase -
  AVFI_RS02245 (AVFI_02245) - 469953..470663 (-) 711 WP_054775635.1 YggS family pyridoxal phosphate-dependent enzyme -
  AVFI_RS02250 (AVFI_02250) pilT 470687..471724 (+) 1038 WP_054775636.1 type IV pilus twitching motility protein PilT Machinery gene
  AVFI_RS02255 (AVFI_02255) pilU 471727..472833 (+) 1107 WP_054775637.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  AVFI_RS02260 (AVFI_02260) ruvX 472982..473407 (-) 426 WP_011261217.1 Holliday junction resolvase RuvX -
  AVFI_RS02265 (AVFI_02265) - 473404..473967 (-) 564 WP_005417575.1 YqgE/AlgH family protein -
  AVFI_RS02270 (AVFI_02270) gshB 474080..475027 (-) 948 WP_017019406.1 glutathione synthase -
  AVFI_RS02275 (AVFI_02275) rsmE 475041..475772 (-) 732 WP_065597616.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  AVFI_RS02280 (AVFI_02280) - 475846..476550 (-) 705 WP_011261220.1 endonuclease -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38122.74 Da        Isoelectric Point: 6.2397

>NTDB_id=659768 AVFI_RS02250 WP_054775636.1 470687..471724(+) (pilT) [Aliivibrio fischeri ATCC 7744 = JCM 18803 = DSM 507]
MDITELLDFSVKQNASDLHLSAGVPPMVRVDGEVRKLTLPDFTHADVHRLVTDIMNDAQRSEFEDKLEVDFSFELPNIGR
FRVNAFNQSRGCAAVFRTIPVDIPSLDQLAAPQVFTDIAKYQKGLVLITGPTGSGKSTTLAAMVDYINENYNKHVLTIED
PIEFVHKNKKCLINQREVNRDTHSFKAALRSALREDPDVILVGELRDQETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGSDKSMVRSMLSESLRAVVAQTLLKRIGGGRIACHEIMIATPAIRNLIREDKVAQMYSIIQTGSSFGMNTMEQNA
KKIIAQGLVDPDEVVKKVEMSSIGF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=659768 AVFI_RS02250 WP_054775636.1 470687..471724(+) (pilT) [Aliivibrio fischeri ATCC 7744 = JCM 18803 = DSM 507]
ATGGACATCACAGAACTACTGGACTTTAGTGTAAAACAAAACGCATCAGATCTACACCTATCTGCAGGAGTTCCTCCTAT
GGTACGTGTGGATGGTGAAGTGCGTAAACTGACATTACCTGATTTTACACATGCCGATGTTCATCGTTTAGTGACTGACA
TTATGAATGATGCTCAGCGTAGTGAATTTGAAGATAAATTAGAAGTCGATTTTTCATTTGAGTTACCTAATATTGGTCGC
TTTCGTGTGAATGCATTTAACCAATCTCGTGGCTGTGCAGCCGTGTTTCGAACTATCCCCGTTGATATTCCGTCATTAGA
TCAATTAGCTGCACCTCAAGTGTTCACCGATATTGCAAAATATCAAAAAGGGTTAGTATTAATCACAGGACCTACTGGTT
CTGGTAAGTCGACAACTTTAGCAGCTATGGTTGATTACATTAACGAGAACTACAACAAGCACGTACTTACGATTGAAGAT
CCGATTGAATTTGTTCATAAGAATAAGAAGTGTTTGATTAACCAACGTGAAGTTAATCGTGATACACATAGTTTTAAAGC
AGCACTTCGTTCAGCTTTGCGTGAAGATCCTGATGTTATTTTAGTTGGTGAATTACGTGATCAAGAGACAATCAGTCTTG
CATTGACTGCCGCAGAAACTGGACACTTGGTATTTGGTACTTTGCACACGAGTTCAGCGGCAAAAACCATTGACCGTATT
ATTGATGTTTTCCCTGGCAGTGATAAATCTATGGTTCGCTCAATGTTATCTGAATCATTAAGAGCGGTAGTAGCACAAAC
CTTACTTAAACGTATTGGTGGTGGTCGTATTGCGTGCCATGAAATTATGATAGCGACACCAGCTATTCGTAACTTAATCC
GTGAAGATAAAGTCGCTCAAATGTATTCAATCATTCAAACGGGTTCTTCTTTTGGTATGAATACTATGGAGCAGAATGCG
AAGAAAATTATTGCTCAAGGTTTGGTTGATCCAGATGAAGTCGTTAAGAAAGTTGAAATGAGCAGCATAGGGTTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

81.739

100

0.817
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

81.739

100

0.817
  pilT Acinetobacter baumannii strain A118

71.903

95.942

0.69
  pilT Acinetobacter baumannii D1279779

71.903

95.942

0.69
  pilT Acinetobacter nosocomialis M2

71.601

95.942

0.687
  pilT Pseudomonas stutzeri DSM 10701

68.437

98.261

0.672
  pilT Acinetobacter baylyi ADP1

70

95.652

0.67
  pilT Pseudomonas aeruginosa PAK

67.353

98.551

0.664
  pilT Legionella pneumophila strain Lp02

67.477

95.362

0.643
  pilT Legionella pneumophila strain ERS1305867

67.477

95.362

0.643
  pilT Neisseria meningitidis 8013

62.573

99.13

0.62
  pilT Neisseria gonorrhoeae MS11

62.281

99.13

0.617
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.968

98.261

0.481
  pilU Pseudomonas stutzeri DSM 10701

41.566

96.232

0.4
  pilU Vibrio cholerae strain A1552

40.308

94.203

0.38