Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   AVFI_RS02255 Genome accession   NZ_CP092712
Coordinates   471727..472833 (+) Length   368 a.a.
NCBI ID   WP_054775637.1    Uniprot ID   -
Organism   Aliivibrio fischeri ATCC 7744 = JCM 18803 = DSM 507     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 466727..477833
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AVFI_RS02220 (AVFI_02220) - 467046..467642 (-) 597 WP_011261211.1 XTP/dITP diphosphatase -
  AVFI_RS02225 (AVFI_02225) - 467653..468084 (-) 432 WP_054775633.1 DUF4426 domain-containing protein -
  AVFI_RS02230 (AVFI_02230) yggU 468154..468441 (-) 288 WP_012533617.1 DUF167 family protein YggU -
  AVFI_RS02235 (AVFI_02235) - 468449..469003 (-) 555 WP_012533297.1 YggT family protein -
  AVFI_RS02240 (AVFI_02240) proC 469052..469870 (-) 819 WP_054775634.1 pyrroline-5-carboxylate reductase -
  AVFI_RS02245 (AVFI_02245) - 469953..470663 (-) 711 WP_054775635.1 YggS family pyridoxal phosphate-dependent enzyme -
  AVFI_RS02250 (AVFI_02250) pilT 470687..471724 (+) 1038 WP_054775636.1 type IV pilus twitching motility protein PilT Machinery gene
  AVFI_RS02255 (AVFI_02255) pilU 471727..472833 (+) 1107 WP_054775637.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  AVFI_RS02260 (AVFI_02260) ruvX 472982..473407 (-) 426 WP_011261217.1 Holliday junction resolvase RuvX -
  AVFI_RS02265 (AVFI_02265) - 473404..473967 (-) 564 WP_005417575.1 YqgE/AlgH family protein -
  AVFI_RS02270 (AVFI_02270) gshB 474080..475027 (-) 948 WP_017019406.1 glutathione synthase -
  AVFI_RS02275 (AVFI_02275) rsmE 475041..475772 (-) 732 WP_065597616.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  AVFI_RS02280 (AVFI_02280) - 475846..476550 (-) 705 WP_011261220.1 endonuclease -
  AVFI_RS02285 (AVFI_02285) - 476642..477142 (-) 501 WP_011261221.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41168.19 Da        Isoelectric Point: 5.4668

>NTDB_id=659769 AVFI_RS02255 WP_054775637.1 471727..472833(+) (pilU) [Aliivibrio fischeri ATCC 7744 = JCM 18803 = DSM 507]
MTLDQILKQMIALKASDIYLTVDAPCLLRVNGELQALGHKQSIESVTALLAEMMDEDRQEEFNESLEANFAIVKEFGRFR
VSAFWQREQPGAVIRRIETKIPSMESLNLPEVMKELAVTKRGLVLVVGATGSGKSTSMAAMTGYRNDNRSGHILTVEDPI
EFVHPHNKCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRNRETMEYAMSFAETGHLCMATLHANNANQALERILH
LVPKESKEQFLFDLSMNLKGVIAQQLVPDMNGNGRHGVYEVLLNTPRVADLIRRGDLHELKSTMAKSRESGMQTFDQSLF
ELVMDKKISEMEALHSADSANDLRLMLKTKRGDAFSSSTLDNVSISLD

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=659769 AVFI_RS02255 WP_054775637.1 471727..472833(+) (pilU) [Aliivibrio fischeri ATCC 7744 = JCM 18803 = DSM 507]
ATGACATTAGATCAAATACTGAAACAAATGATCGCTTTAAAAGCATCGGATATTTACTTAACGGTTGATGCTCCTTGTTT
ACTGAGAGTGAATGGTGAACTTCAAGCTTTAGGCCACAAACAGAGTATTGAATCAGTCACTGCTTTGCTTGCTGAGATGA
TGGATGAAGATCGCCAAGAAGAGTTTAATGAAAGCTTAGAAGCGAACTTTGCCATTGTGAAAGAATTTGGTCGCTTTCGT
GTGAGTGCTTTTTGGCAGCGAGAGCAACCTGGTGCAGTGATACGTCGTATTGAAACCAAAATTCCGTCCATGGAGTCACT
AAATCTTCCAGAAGTAATGAAAGAATTAGCCGTTACTAAGCGTGGATTAGTATTAGTGGTGGGGGCTACGGGTTCAGGTA
AATCAACGTCAATGGCGGCGATGACGGGTTATCGAAATGACAACCGAAGTGGTCATATCTTAACGGTAGAAGACCCAATT
GAGTTTGTGCATCCTCATAATAAATGCATCGTGACTCAGCGTGAAGTTGGCCTTGATACTGAAAGTTATGAAGTGGCATT
AAAAAACTCGTTACGCCAAGCGCCAGATATGATTTTAATTGGTGAGATCCGTAATCGAGAGACAATGGAATACGCCATGA
GCTTTGCTGAAACGGGGCATTTATGCATGGCAACATTACATGCAAATAACGCTAACCAAGCATTAGAGCGAATTTTGCAC
CTTGTTCCTAAAGAGAGTAAAGAGCAGTTTTTGTTTGATTTATCCATGAACTTAAAAGGGGTCATTGCACAGCAGTTAGT
TCCGGATATGAATGGTAATGGTCGTCATGGCGTTTATGAAGTATTACTAAACACGCCACGCGTTGCAGACTTGATCCGTC
GTGGTGATTTGCATGAACTTAAATCGACCATGGCAAAATCACGTGAGTCGGGTATGCAGACGTTTGATCAGAGCTTATTT
GAACTGGTAATGGATAAGAAAATATCAGAAATGGAAGCGCTGCACAGTGCGGATTCGGCTAATGATTTACGCCTGATGTT
GAAAACAAAAAGAGGGGATGCGTTCTCTTCATCTACATTGGATAATGTGTCGATATCGTTAGACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(6-268)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

75.815

100

0.758
  pilU Pseudomonas stutzeri DSM 10701

57.429

95.109

0.546
  pilU Acinetobacter baylyi ADP1

53.276

95.38

0.508
  pilT Legionella pneumophila strain ERS1305867

41.691

93.207

0.389
  pilT Legionella pneumophila strain Lp02

41.691

93.207

0.389
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.216

90.761

0.383
  pilT Acinetobacter baumannii D1279779

41.108

93.207

0.383
  pilT Acinetobacter baumannii strain A118

41.108

93.207

0.383
  pilT Acinetobacter nosocomialis M2

41.108

93.207

0.383
  pilT Pseudomonas aeruginosa PAK

42.249

89.402

0.378
  pilT Pseudomonas stutzeri DSM 10701

41.641

89.402

0.372
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.538

88.315

0.367
  pilT Vibrio cholerae strain A1552

41.538

88.315

0.367