Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   MKR81_RS02365 Genome accession   NZ_CP092682
Coordinates   489104..490144 (+) Length   346 a.a.
NCBI ID   WP_005425810.1    Uniprot ID   A0A9X3MNX0
Organism   Vibrio campbellii strain IFL1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 484104..495144
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MKR81_RS02330 (MKR81_02330) hemW 484125..485297 (-) 1173 WP_240304998.1 radical SAM family heme chaperone HemW -
  MKR81_RS02335 (MKR81_02335) - 485316..485918 (-) 603 WP_005436525.1 XTP/dITP diphosphatase -
  MKR81_RS02340 (MKR81_02340) - 486024..486455 (-) 432 WP_005425804.1 DUF4426 domain-containing protein -
  MKR81_RS02345 (MKR81_02345) yggU 486515..486805 (-) 291 WP_240304999.1 DUF167 family protein YggU -
  MKR81_RS02350 (MKR81_02350) - 486805..487362 (-) 558 WP_240305000.1 YggT family protein -
  MKR81_RS02355 (MKR81_02355) proC 487416..488234 (-) 819 WP_005425807.1 pyrroline-5-carboxylate reductase -
  MKR81_RS02360 (MKR81_02360) - 488365..489075 (-) 711 WP_005425809.1 YggS family pyridoxal phosphate-dependent enzyme -
  MKR81_RS02365 (MKR81_02365) pilT 489104..490144 (+) 1041 WP_005425810.1 type IV pilus twitching motility protein PilT Machinery gene
  MKR81_RS02370 (MKR81_02370) pilU 490176..491282 (+) 1107 WP_228175557.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  MKR81_RS02375 (MKR81_02375) ruvX 491344..491769 (-) 426 WP_005425820.1 Holliday junction resolvase RuvX -
  MKR81_RS02380 (MKR81_02380) - 491817..492380 (-) 564 WP_038892068.1 YqgE/AlgH family protein -
  MKR81_RS02385 (MKR81_02385) gshB 492510..493460 (-) 951 WP_005425823.1 glutathione synthase -
  MKR81_RS02390 (MKR81_02390) rsmE 493475..494206 (-) 732 WP_240305001.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  MKR81_RS02395 (MKR81_02395) endA 494353..495048 (-) 696 WP_005425828.1 deoxyribonuclease I -

Sequence

Protein


Download         Length: 346 a.a.        Molecular weight: 38634.12 Da        Isoelectric Point: 6.5043

>NTDB_id=659656 MKR81_RS02365 WP_005425810.1 489104..490144(+) (pilT) [Vibrio campbellii strain IFL1]
MDITELLDFSVKHNASDLHLSAGVPPMVRIDGDVRKLGIPAFTHQEVHRLVFEIMNDAQRSEFEEKLEVDFSFELHNVGR
FRVNAFNQSRGCAAVFRTIPSSIPTLEELEAPEIFRKIANAEKGLVLVTGPTGSGKSTTLAAIVDYINRNHNKHVLTIED
PIEFVHTNNKCLINQREVHRDTHSFQNALRSALREDPDVILVGEMRDKETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGSDKDMVRSMLSESLRSVIAQKLLKRNGGGRIACHEIMMATPAIRNLIREDKVAQMYSIIQTGAAHGMQTMEQNA
RQLMAQGMVSREEVDKKVEIEAQQFS

Nucleotide


Download         Length: 1041 bp        

>NTDB_id=659656 MKR81_RS02365 WP_005425810.1 489104..490144(+) (pilT) [Vibrio campbellii strain IFL1]
ATGGATATCACTGAGTTACTGGATTTTAGTGTAAAACATAATGCTTCGGATCTACATCTTTCTGCAGGTGTACCACCAAT
GGTTCGTATTGATGGTGATGTAAGAAAACTGGGCATTCCAGCGTTTACACACCAAGAAGTGCACCGTTTGGTTTTTGAAA
TCATGAACGATGCGCAGCGCAGTGAGTTCGAAGAAAAACTCGAAGTCGACTTTTCATTTGAATTGCATAACGTTGGCCGT
TTTCGTGTCAACGCTTTCAACCAATCTCGCGGTTGTGCGGCGGTGTTCCGTACTATCCCAAGTAGCATTCCAACACTGGA
AGAGCTAGAAGCTCCTGAGATCTTCAGAAAAATCGCCAATGCGGAAAAAGGTTTGGTACTGGTTACTGGCCCAACGGGTT
CTGGTAAATCGACTACCTTGGCTGCCATTGTTGACTACATTAACCGTAATCACAATAAGCACGTCCTAACAATTGAAGAT
CCGATTGAATTTGTTCACACCAACAATAAGTGTCTGATCAACCAACGTGAAGTACACCGTGATACACACAGCTTCCAAAA
CGCGCTACGCAGCGCGCTGCGTGAAGACCCGGATGTGATTCTAGTTGGTGAGATGCGTGATAAAGAAACCATCAGTTTGG
CGCTGACGGCTGCTGAAACGGGTCACCTTGTTTTCGGTACGCTGCACACTAGCTCGGCAGCGAAAACCATCGACCGTATT
ATTGACGTCTTCCCTGGTAGCGACAAAGACATGGTGCGTTCGATGCTGTCTGAATCATTGCGTTCGGTTATCGCGCAAAA
ACTTCTGAAACGTAACGGCGGCGGTCGTATCGCGTGTCATGAAATAATGATGGCAACGCCTGCGATCCGTAACCTTATTC
GTGAAGACAAAGTCGCGCAGATGTATTCGATCATTCAGACTGGTGCGGCGCATGGTATGCAGACCATGGAGCAGAATGCT
CGTCAATTGATGGCGCAAGGCATGGTATCGCGTGAAGAAGTGGATAAGAAAGTTGAAATTGAAGCGCAGCAATTCTCGTA
A

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

86.377

99.711

0.861
  pilT Vibrio cholerae strain A1552

86.377

99.711

0.861
  pilT Acinetobacter baumannii D1279779

72.121

95.376

0.688
  pilT Acinetobacter baumannii strain A118

72.121

95.376

0.688
  pilT Acinetobacter nosocomialis M2

71.818

95.376

0.685
  pilT Acinetobacter baylyi ADP1

71.515

95.376

0.682
  pilT Pseudomonas aeruginosa PAK

68.824

98.266

0.676
  pilT Pseudomonas stutzeri DSM 10701

69.027

97.977

0.676
  pilT Legionella pneumophila strain Lp02

67.674

95.665

0.647
  pilT Legionella pneumophila strain ERS1305867

67.674

95.665

0.647
  pilT Neisseria meningitidis 8013

64.118

98.266

0.63
  pilT Neisseria gonorrhoeae MS11

63.824

98.266

0.627
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.857

100

0.503
  pilU Vibrio cholerae strain A1552

41.617

96.532

0.402
  pilU Pseudomonas stutzeri DSM 10701

40.419

96.532

0.39