Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   MKR81_RS02370 Genome accession   NZ_CP092682
Coordinates   490176..491282 (+) Length   368 a.a.
NCBI ID   WP_228175557.1    Uniprot ID   -
Organism   Vibrio campbellii strain IFL1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 485176..496282
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MKR81_RS02335 (MKR81_02335) - 485316..485918 (-) 603 WP_005436525.1 XTP/dITP diphosphatase -
  MKR81_RS02340 (MKR81_02340) - 486024..486455 (-) 432 WP_005425804.1 DUF4426 domain-containing protein -
  MKR81_RS02345 (MKR81_02345) yggU 486515..486805 (-) 291 WP_240304999.1 DUF167 family protein YggU -
  MKR81_RS02350 (MKR81_02350) - 486805..487362 (-) 558 WP_240305000.1 YggT family protein -
  MKR81_RS02355 (MKR81_02355) proC 487416..488234 (-) 819 WP_005425807.1 pyrroline-5-carboxylate reductase -
  MKR81_RS02360 (MKR81_02360) - 488365..489075 (-) 711 WP_005425809.1 YggS family pyridoxal phosphate-dependent enzyme -
  MKR81_RS02365 (MKR81_02365) pilT 489104..490144 (+) 1041 WP_005425810.1 type IV pilus twitching motility protein PilT Machinery gene
  MKR81_RS02370 (MKR81_02370) pilU 490176..491282 (+) 1107 WP_228175557.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  MKR81_RS02375 (MKR81_02375) ruvX 491344..491769 (-) 426 WP_005425820.1 Holliday junction resolvase RuvX -
  MKR81_RS02380 (MKR81_02380) - 491817..492380 (-) 564 WP_038892068.1 YqgE/AlgH family protein -
  MKR81_RS02385 (MKR81_02385) gshB 492510..493460 (-) 951 WP_005425823.1 glutathione synthase -
  MKR81_RS02390 (MKR81_02390) rsmE 493475..494206 (-) 732 WP_240305001.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  MKR81_RS02395 (MKR81_02395) endA 494353..495048 (-) 696 WP_005425828.1 deoxyribonuclease I -
  MKR81_RS02400 (MKR81_02400) - 495199..495696 (-) 498 WP_240305002.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41319.48 Da        Isoelectric Point: 6.7731

>NTDB_id=659657 MKR81_RS02370 WP_228175557.1 490176..491282(+) (pilU) [Vibrio campbellii strain IFL1]
MDLNKFLEGMLALKASDLYITVGAPILFRVDGELRPQGDKLTENDVALLLDSAMEPERRQEFRKSRESNFAIVRDCGRFR
VSAFFQRELPGAVIRRIETNIPTFEQLKLPLVLQDLAIAKRGLVLVVGATGSGKSTTMAAMTGYRNNNKTGHILTVEDPI
EFVHEHKRCIVTQREVGLDTDSYEVALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQALERILH
LVPKDQKDQFLFDLSMNLKGVVGQQLIRDKNGQGRHGVFEILLNSPRVSDLIRRGDLHELKATMARSNEFGMLTFDQSLY
KLVMQGKISEEDALHSADSANDLRLMLKTQRGEAFSTGTLANVKIDMD

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=659657 MKR81_RS02370 WP_228175557.1 490176..491282(+) (pilU) [Vibrio campbellii strain IFL1]
ATGGATCTGAATAAATTTCTCGAAGGCATGTTGGCGTTAAAAGCGTCGGATCTCTACATCACTGTCGGTGCTCCGATTTT
GTTTCGTGTGGATGGCGAATTGCGCCCGCAAGGTGATAAGCTGACTGAAAATGATGTCGCTTTGTTACTTGACAGTGCCA
TGGAACCAGAGCGACGTCAGGAGTTTCGTAAAAGCCGTGAGTCTAACTTTGCCATTGTCCGTGATTGCGGTCGTTTCCGT
GTCAGTGCCTTTTTCCAACGTGAATTACCGGGAGCCGTTATTCGTCGTATTGAAACCAATATTCCGACGTTTGAGCAACT
TAAGTTACCTTTGGTACTGCAAGATCTTGCTATTGCCAAGCGTGGTTTAGTGCTGGTGGTTGGTGCCACTGGTTCTGGTA
AGTCGACCACTATGGCGGCGATGACGGGTTATCGCAACAACAACAAAACGGGTCATATCCTGACGGTAGAAGACCCGATT
GAATTTGTGCACGAACATAAGCGTTGCATCGTGACACAGCGTGAAGTTGGCTTAGACACCGACAGCTATGAAGTGGCGTT
GAAAAACTCGTTACGTCAGGCGCCAGATATGATTTTGATTGGTGAGATCCGTAGCCGAGAAACGATGGAATACGCGATGA
CCTTTGCTGAGACTGGTCACTTGTGTATGGCGACACTGCACGCCAACAACGCAAACCAAGCGCTGGAGCGTATTTTGCAT
CTAGTGCCAAAAGATCAGAAAGATCAGTTCCTGTTTGATCTGTCGATGAACTTAAAAGGTGTAGTAGGTCAGCAGTTGAT
CCGTGATAAGAACGGTCAGGGGCGTCATGGCGTGTTTGAGATCTTATTAAATAGCCCGCGTGTATCCGATCTGATCCGTC
GAGGCGATCTTCATGAGTTGAAAGCAACCATGGCTCGATCCAATGAATTTGGGATGTTGACCTTCGACCAATCGCTCTAC
AAGTTAGTGATGCAAGGTAAGATCAGTGAAGAAGATGCATTGCACAGTGCGGACTCTGCCAACGATCTGCGTTTGATGTT
GAAGACGCAACGTGGTGAAGCATTCTCTACAGGCACGCTGGCGAACGTTAAGATCGATATGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(14-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

81.522

100

0.815
  pilU Pseudomonas stutzeri DSM 10701

58.757

96.196

0.565
  pilU Acinetobacter baylyi ADP1

55.556

95.38

0.53
  pilT Acinetobacter baylyi ADP1

41.57

93.478

0.389
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44

88.315

0.389
  pilT Acinetobacter nosocomialis M2

41.888

92.12

0.386
  pilT Acinetobacter baumannii D1279779

41.888

92.12

0.386
  pilT Acinetobacter baumannii strain A118

41.888

92.12

0.386
  pilT Legionella pneumophila strain Lp02

41.888

92.12

0.386
  pilT Legionella pneumophila strain ERS1305867

41.888

92.12

0.386
  pilT Pseudomonas aeruginosa PAK

41.593

92.12

0.383
  pilT Pseudomonas stutzeri DSM 10701

40.643

92.935

0.378
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.018

90.761

0.372
  pilT Vibrio cholerae strain A1552

41.018

90.761

0.372