Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   MJ615_RS20575 Genome accession   NZ_CP092648
Coordinates   4226697..4227677 (-) Length   326 a.a.
NCBI ID   WP_020801960.1    Uniprot ID   A0A331PSC5
Organism   Klebsiella pneumoniae strain KP7728     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4221697..4232677
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MJ615_RS20545 endA 4221987..4222694 (+) 708 WP_002916589.1 deoxyribonuclease I -
  MJ615_RS20550 rsmE 4222786..4223517 (+) 732 WP_004144776.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  MJ615_RS20555 gshB 4223538..4224488 (+) 951 WP_002916600.1 glutathione synthase -
  MJ615_RS20560 - 4224663..4225226 (+) 564 WP_002916603.1 YqgE/AlgH family protein -
  MJ615_RS20565 ruvX 4225226..4225642 (+) 417 WP_002916605.1 Holliday junction resolvase RuvX -
  MJ615_RS20570 - 4225695..4226348 (-) 654 WP_002916607.1 LuxR family transcriptional regulator -
  MJ615_RS20575 pilT 4226697..4227677 (-) 981 WP_020801960.1 type IV pilus twitching motility protein PilT Machinery gene
  MJ615_RS20580 - 4227694..4228395 (+) 702 WP_004144780.1 YggS family pyridoxal phosphate-dependent enzyme -
  MJ615_RS20585 - 4228416..4228982 (+) 567 WP_002916613.1 YggT family protein -
  MJ615_RS20590 yggU 4228979..4229269 (+) 291 WP_002916614.1 DUF167 family protein YggU -
  MJ615_RS20595 - 4229282..4229875 (+) 594 WP_002916615.1 XTP/dITP diphosphatase -
  MJ615_RS20600 hemW 4229868..4231007 (+) 1140 WP_004889776.1 radical SAM family heme chaperone HemW -
  MJ615_RS20605 - 4231300..4232346 (+) 1047 WP_004185615.1 sugar ABC transporter substrate-binding protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35284.75 Da        Isoelectric Point: 6.8575

>NTDB_id=659567 MJ615_RS20575 WP_020801960.1 4226697..4227677(-) (pilT) [Klebsiella pneumoniae strain KP7728]
MKLEEIVALSVKHNVSDLHLCNSAAPRWRRQGRLEPAPFPAPDIANLLNDWLDAAQLLHWQEHGQIDFALTLACGARLRA
SAFAHTRGISLVLRLLPEQCPRLDTLGAPPALSELLAEESGLLLVTGATGSGKSTTLAAMVGHLNQHLDGHILTLEDPVE
FIHHSERCLIQQREVGRHCPSFAAALRVALRQDPDVILLGELRDSETIRLALTAAETGHLVMATLHTRGAAPAVERLIDV
FPAEEKDQVRSQLAGSLCAVLAQKLLPARQGGRVALYELLVNTPAVANLIREGKVHQLPGVMQTGMQAGMLTFTQSFQQR
VAAGAL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=659567 MJ615_RS20575 WP_020801960.1 4226697..4227677(-) (pilT) [Klebsiella pneumoniae strain KP7728]
ATGAAGCTGGAAGAAATCGTAGCCCTTAGTGTAAAGCATAATGTCTCTGATCTACACCTGTGCAATTCCGCCGCACCACG
CTGGCGGCGGCAGGGCAGGCTGGAGCCCGCGCCCTTTCCCGCGCCGGATATTGCGAATTTACTCAACGACTGGCTCGATG
CCGCACAGTTACTGCACTGGCAGGAACACGGCCAGATTGACTTTGCGCTGACCCTGGCCTGCGGTGCGCGGCTGCGCGCC
AGCGCCTTCGCCCATACGCGTGGGATATCGCTGGTGCTGCGCCTCCTTCCTGAACAGTGCCCGCGTCTGGACACGCTCGG
CGCCCCGCCTGCGCTGAGCGAACTGCTGGCGGAAGAGAGTGGTTTGCTGTTGGTCACCGGAGCGACGGGCAGCGGCAAGT
CGACCACCCTGGCGGCAATGGTGGGGCACCTCAATCAGCATCTTGATGGCCATATTCTGACGCTTGAAGATCCGGTGGAA
TTTATTCATCACAGCGAGCGCTGTCTGATCCAGCAGCGGGAAGTTGGCCGTCACTGTCCTTCGTTCGCCGCGGCGCTCCG
CGTGGCGCTGCGCCAGGATCCGGATGTGATCCTGCTCGGGGAGCTACGCGACAGCGAAACCATTCGCCTGGCGTTAACCG
CGGCGGAGACCGGGCACCTGGTAATGGCGACATTACATACCCGCGGCGCGGCGCCGGCGGTGGAGAGATTGATCGATGTC
TTTCCGGCGGAGGAGAAAGATCAGGTTCGTAGCCAGCTTGCCGGAAGCCTGTGCGCGGTGCTGGCGCAAAAATTGTTACC
TGCACGTCAGGGCGGGAGAGTTGCGCTATACGAGCTTCTGGTCAACACGCCAGCGGTGGCGAATTTGATCCGTGAAGGAA
AAGTACATCAATTGCCTGGTGTGATGCAGACCGGGATGCAGGCCGGAATGTTGACCTTTACCCAGAGTTTTCAGCAACGT
GTCGCTGCAGGCGCGCTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A331PSC5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

51.368

100

0.518
  pilT Vibrio cholerae strain A1552

51.368

100

0.518
  pilT Acinetobacter baylyi ADP1

48.936

100

0.494
  pilT Neisseria meningitidis 8013

48.171

100

0.485
  pilT Acinetobacter baumannii D1279779

48.024

100

0.485
  pilT Acinetobacter baumannii strain A118

48.024

100

0.485
  pilT Neisseria gonorrhoeae MS11

47.866

100

0.482
  pilT Acinetobacter nosocomialis M2

47.72

100

0.482
  pilT Legionella pneumophila strain ERS1305867

48.148

99.387

0.479
  pilT Legionella pneumophila strain Lp02

48.148

99.387

0.479
  pilT Pseudomonas stutzeri DSM 10701

47.401

100

0.475
  pilT Pseudomonas aeruginosa PAK

46.789

100

0.469
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.795

97.239

0.436
  pilU Vibrio cholerae strain A1552

41.049

99.387

0.408
  pilU Pseudomonas stutzeri DSM 10701

39.394

100

0.399
  pilU Acinetobacter baylyi ADP1

39.875

98.466

0.393