Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   MKK01_RS03645 Genome accession   NZ_CP092632
Coordinates   739958..740938 (+) Length   326 a.a.
NCBI ID   WP_042948086.1    Uniprot ID   A0A2V3KQC4
Organism   Klebsiella variicola subsp. variicola strain ML.9ba2     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 734958..745938
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MKK01_RS03610 (MKK01_03610) - 735494..735820 (+) 327 WP_012967224.1 YggL family protein -
  MKK01_RS03615 (MKK01_03615) - 735871..736590 (+) 720 WP_012540560.1 DUF2884 domain-containing protein -
  MKK01_RS03620 (MKK01_03620) hemW 736631..737767 (-) 1137 WP_016161832.1 radical SAM family heme chaperone HemW -
  MKK01_RS03625 (MKK01_03625) - 737760..738353 (-) 594 WP_240289623.1 XTP/dITP diphosphatase -
  MKK01_RS03630 (MKK01_03630) yggU 738366..738656 (-) 291 WP_008806468.1 DUF167 family protein YggU -
  MKK01_RS03635 (MKK01_03635) - 738653..739219 (-) 567 WP_004205182.1 YggT family protein -
  MKK01_RS03640 (MKK01_03640) - 739240..739941 (-) 702 WP_004205181.1 YggS family pyridoxal phosphate-dependent enzyme -
  MKK01_RS03645 (MKK01_03645) pilT 739958..740938 (+) 981 WP_042948086.1 type IV pilus twitching motility protein PilT Machinery gene
  MKK01_RS03650 (MKK01_03650) - 741287..741940 (+) 654 WP_086631387.1 LuxR family transcriptional regulator -
  MKK01_RS03655 (MKK01_03655) ruvX 741993..742409 (-) 417 WP_012540565.1 Holliday junction resolvase RuvX -
  MKK01_RS03660 (MKK01_03660) - 742409..742972 (-) 564 WP_012540566.1 YqgE/AlgH family protein -
  MKK01_RS03665 (MKK01_03665) gshB 743147..744097 (-) 951 WP_008806462.1 glutathione synthase -
  MKK01_RS03670 (MKK01_03670) rsmE 744118..744849 (-) 732 WP_008806461.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  MKK01_RS03675 (MKK01_03675) endA 744941..745648 (-) 708 WP_008806460.1 deoxyribonuclease I -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35424.04 Da        Isoelectric Point: 6.7658

>NTDB_id=659399 MKK01_RS03645 WP_042948086.1 739958..740938(+) (pilT) [Klebsiella variicola subsp. variicola strain ML.9ba2]
MKLEEIVALSVKHNVSDLHLCNSAAPRWRRQGKLEPAPFPAPDIPDLLNCWLDAAQLQHWQEQGQIDFALTLACGLRLRA
SAFAHTRGISLVLRLLPEQCPRLETLGAPPALSELLAEESGLLLVTGATGSGKSTTLAAMVGHLNQRLDGHILTLEDPVE
FIHHSERCLILQREIGRHCPSFAAALRVALRQDPDVILLGELRDSETIRLALTAAETGHLVMATLHTRGAAPAVERLIDV
FPAEEKDQVRSQLAGSLCAVLAQKLLPARQGGRVALYELLVNTPAVANLIREGKVHQLPGIMQTGMQAGMLTFTQSFQQR
VAAGEL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=659399 MKK01_RS03645 WP_042948086.1 739958..740938(+) (pilT) [Klebsiella variicola subsp. variicola strain ML.9ba2]
ATGAAGCTGGAAGAAATCGTAGCCCTTAGTGTAAAGCATAATGTCTCCGATCTACACCTGTGCAATTCCGCCGCACCGCG
CTGGCGGCGGCAGGGCAAGCTGGAGCCCGCCCCGTTTCCCGCGCCGGATATCCCGGATTTACTCAACTGCTGGCTTGATG
CCGCACAGTTACAGCATTGGCAGGAACAGGGCCAGATTGACTTTGCGCTGACCCTGGCCTGCGGTTTGCGGCTGCGCGCC
AGCGCTTTCGCCCATACGCGTGGAATATCGCTGGTGCTACGCCTTCTGCCTGAACAGTGCCCGCGTCTGGAGACGCTGGG
CGCCCCGCCTGCGCTGAGCGAGCTGCTGGCGGAAGAGAGTGGTTTGCTGTTGGTCACCGGAGCGACGGGCAGCGGCAAGT
CGACCACCCTGGCGGCGATGGTGGGGCATCTCAACCAGCGTCTTGATGGCCATATTCTGACCCTGGAGGACCCGGTGGAG
TTTATTCATCACAGCGAGCGCTGCCTGATCCTGCAGCGGGAGATTGGCCGTCATTGTCCTTCGTTCGCCGCGGCGCTCCG
CGTGGCGCTGCGCCAGGATCCGGATGTGATCCTGCTCGGGGAGCTACGCGACAGCGAAACCATTCGTCTGGCGTTAACGG
CTGCGGAGACCGGACATCTGGTAATGGCGACATTACATACCCGCGGCGCGGCGCCGGCGGTGGAGAGATTGATCGATGTC
TTTCCGGCAGAGGAGAAAGATCAGGTTCGTAGTCAGCTGGCCGGAAGCCTGTGCGCGGTGCTGGCGCAAAAATTGTTGCC
TGCACGTCAGGGCGGCAGAGTTGCGCTATACGAGCTTTTGGTCAATACCCCCGCAGTAGCTAACTTGATCCGTGAAGGAA
AAGTGCACCAGTTGCCCGGCATAATGCAGACCGGGATGCAGGCCGGAATGCTGACATTTACGCAGAGTTTTCAGCAACGT
GTCGCCGCAGGCGAGCTGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2V3KQC4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

51.682

100

0.518
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

51.682

100

0.518
  pilT Acinetobacter baylyi ADP1

48.012

100

0.482
  pilT Neisseria meningitidis 8013

47.561

100

0.479
  pilT Acinetobacter baumannii strain A118

47.706

100

0.479
  pilT Acinetobacter baumannii D1279779

47.706

100

0.479
  pilT Acinetobacter nosocomialis M2

47.401

100

0.475
  pilT Neisseria gonorrhoeae MS11

47.256

100

0.475
  pilT Legionella pneumophila strain ERS1305867

47.531

99.387

0.472
  pilT Legionella pneumophila strain Lp02

47.531

99.387

0.472
  pilT Pseudomonas stutzeri DSM 10701

46.789

100

0.469
  pilT Pseudomonas aeruginosa PAK

45.872

100

0.46
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.849

97.239

0.426
  pilU Vibrio cholerae strain A1552

40.122

100

0.405
  pilU Pseudomonas stutzeri DSM 10701

40

100

0.405
  pilU Acinetobacter baylyi ADP1

38.941

98.466

0.383