Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   MJ899_RS03745 Genome accession   NZ_CP092627
Coordinates   780289..781269 (+) Length   326 a.a.
NCBI ID   WP_017465459.1    Uniprot ID   A0A5Z0IGI2
Organism   Salmonella enterica subsp. enterica serovar Thompson strain MFDS1011657     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 775289..786269
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MJ899_RS03715 (MJ899_03710) - 775866..776873 (+) 1008 WP_000252195.1 DUF1202 domain-containing protein -
  MJ899_RS03720 (MJ899_03715) hemW 776965..778101 (-) 1137 WP_017465458.1 radical SAM family heme chaperone HemW -
  MJ899_RS03725 (MJ899_03720) - 778094..778687 (-) 594 WP_001174769.1 XTP/dITP diphosphatase -
  MJ899_RS03730 (MJ899_03725) yggU 778695..778985 (-) 291 WP_001277203.1 DUF167 family protein YggU -
  MJ899_RS03735 (MJ899_03730) - 778982..779548 (-) 567 WP_001094848.1 YggT family protein -
  MJ899_RS03740 (MJ899_03735) - 779567..780271 (-) 705 WP_000997812.1 YggS family pyridoxal phosphate-dependent enzyme -
  MJ899_RS03745 (MJ899_03740) pilT 780289..781269 (+) 981 WP_017465459.1 type IV pilus twitching motility protein PilT Machinery gene
  MJ899_RS03750 (MJ899_03745) - 781402..782088 (+) 687 WP_000098321.1 IclR family transcriptional regulator -
  MJ899_RS03755 (MJ899_03750) ruvX 782135..782551 (-) 417 WP_001285491.1 Holliday junction resolvase RuvX -
  MJ899_RS03760 (MJ899_03755) - 782551..783114 (-) 564 WP_001053167.1 YqgE/AlgH family protein -
  MJ899_RS03765 (MJ899_03760) gshB 783330..784277 (-) 948 WP_000593243.1 glutathione synthase -
  MJ899_RS03770 (MJ899_03765) rsmE 784297..785028 (-) 732 WP_023203985.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  MJ899_RS03775 (MJ899_03770) endA 785105..785812 (-) 708 WP_000286133.1 deoxyribonuclease I -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 36140.34 Da        Isoelectric Point: 7.5355

>NTDB_id=659242 MJ899_RS03745 WP_017465459.1 780289..781269(+) (pilT) [Salmonella enterica subsp. enterica serovar Thompson strain MFDS1011657]
MNMEEIVTLSVKHNVSDLHLCNAWPARWRKQGRMETAPFTAPDVDRLLLDWLNDAQQYQWRTHGQLDFAVSLSGTRRLRA
SAFTHQQGTSLAIRLLPERCPDLAEIQTPPIVPALLASENGLILVTGATGCGKSTTLAAMVGHLNQHADKHILTLEDPIE
YRYTSKRCLIQQREIGQHCATFAAGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKEPVRSQLAGSLRAVLSQKLEVDRQDGRVALFELLINTPATGNLIREGKLHQLAHVIQTGQQQGMMTFAQSAQWR
QVQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=659242 MJ899_RS03745 WP_017465459.1 780289..781269(+) (pilT) [Salmonella enterica subsp. enterica serovar Thompson strain MFDS1011657]
ATGAATATGGAAGAAATTGTGACGCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGTAACGCCTGGCCTGCGCG
ATGGCGCAAACAAGGACGCATGGAGACCGCGCCTTTTACCGCGCCTGATGTCGACAGGCTTCTGCTTGATTGGCTTAATG
ACGCGCAACAATATCAGTGGCGAACGCACGGTCAGCTTGATTTCGCCGTTTCGCTGTCTGGCACGCGGCGCCTGCGCGCC
AGTGCGTTCACACATCAACAGGGAACGTCGCTGGCGATACGGCTATTGCCCGAGCGTTGTCCTGATCTGGCGGAAATCCA
GACGCCGCCGATAGTACCCGCACTGCTTGCCAGCGAGAACGGACTGATTCTGGTCACTGGCGCTACTGGCTGCGGGAAAT
CCACTACTCTGGCGGCGATGGTGGGACATCTTAATCAACATGCCGATAAACACATCCTGACGCTGGAAGATCCTATTGAG
TACCGTTACACCAGCAAGCGTTGCCTGATACAGCAGCGGGAAATCGGCCAGCATTGCGCTACTTTTGCCGCCGGGCTGCG
CGCGGCGTTGCGTGAAGATCCCGATGTCATTTTGCTGGGAGAGCTGCGTGACAGCGAGACCATTCGCCTGGCGCTGACGG
CGGCGGAAACGGGGCATCTGGTACTGGCAACCCTGCATACTCGCGGTGCGGCGCAGGCGGTGGAAAGATTAGTGGATAGT
TTTCCGGCGCAGGAAAAAGAACCGGTACGCAGCCAACTGGCGGGTAGTCTGCGCGCCGTACTATCACAAAAACTGGAGGT
CGACAGACAGGATGGGCGCGTGGCGTTGTTTGAATTACTGATTAATACGCCAGCCACAGGGAATTTGATCCGTGAAGGCA
AACTCCATCAGTTGGCCCACGTGATACAAACCGGGCAGCAGCAGGGGATGATGACATTCGCCCAGAGCGCGCAATGGCGT
CAGGTGCAGGGACGGCTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(75-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5Z0IGI2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.318

100

0.485
  pilT Vibrio cholerae strain A1552

48.318

100

0.485
  pilT Neisseria meningitidis 8013

47.256

100

0.475
  pilT Neisseria gonorrhoeae MS11

46.951

100

0.472
  pilT Acinetobacter baylyi ADP1

44.954

100

0.451
  pilT Acinetobacter baumannii strain A118

44.648

100

0.448
  pilT Acinetobacter baumannii D1279779

44.648

100

0.448
  pilT Acinetobacter nosocomialis M2

45.455

97.853

0.445
  pilT Pseudomonas aeruginosa PAK

45.886

96.933

0.445
  pilT Pseudomonas stutzeri DSM 10701

45.886

96.933

0.445
  pilT Legionella pneumophila strain ERS1305867

44.654

97.546

0.436
  pilT Legionella pneumophila strain Lp02

44.654

97.546

0.436
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.138

97.546

0.411
  pilU Vibrio cholerae strain A1552

38.871

97.853

0.38
  pilU Acinetobacter baylyi ADP1

37.152

99.08

0.368
  pilU Pseudomonas stutzeri DSM 10701

35.65

100

0.362