Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   MKS89_RS13050 Genome accession   NZ_CP092587
Coordinates   2926523..2927518 (-) Length   331 a.a.
NCBI ID   WP_072958266.1    Uniprot ID   A0A1M5A6V5
Organism   Vibrio gazogenes strain PB1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2921523..2932518
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MKS89_RS13020 (MKS89_13035) metK 2921618..2922772 (+) 1155 WP_072958279.1 methionine adenosyltransferase -
  MKS89_RS13025 (MKS89_13040) - 2922967..2923479 (+) 513 WP_077316377.1 SprT family zinc-dependent metalloprotease -
  MKS89_RS13030 (MKS89_13045) rsmE 2923722..2924453 (+) 732 WP_072958277.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  MKS89_RS13035 (MKS89_13050) gshB 2924483..2925430 (+) 948 WP_072958274.1 glutathione synthase -
  MKS89_RS13040 (MKS89_13055) - 2925472..2926035 (+) 564 WP_072958271.1 YqgE/AlgH family protein -
  MKS89_RS13045 (MKS89_13060) ruvX 2926077..2926499 (+) 423 WP_072958269.1 Holliday junction resolvase RuvX -
  MKS89_RS13050 (MKS89_13065) pilT 2926523..2927518 (-) 996 WP_072958266.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  MKS89_RS13055 (MKS89_13070) - 2927649..2928356 (+) 708 WP_072958263.1 YggS family pyridoxal phosphate-dependent enzyme -
  MKS89_RS13060 (MKS89_13075) proC 2928383..2929204 (+) 822 WP_072958259.1 pyrroline-5-carboxylate reductase -
  MKS89_RS13065 (MKS89_13080) - 2929256..2929813 (+) 558 WP_072958256.1 YggT family protein -
  MKS89_RS13070 (MKS89_13085) yggU 2929813..2930100 (+) 288 WP_072958253.1 DUF167 family protein YggU -
  MKS89_RS13075 (MKS89_13090) - 2930301..2931764 (+) 1464 WP_072958251.1 UbiD family decarboxylase -

Sequence

Protein


Download         Length: 331 a.a.        Molecular weight: 36701.13 Da        Isoelectric Point: 7.2375

>NTDB_id=659188 MKS89_RS13050 WP_072958266.1 2926523..2927518(-) (pilT) [Vibrio gazogenes strain PB1]
MDISTLLALSVKQNASDLHLSANHPPMLRIDGGLRKVACETLSSQTILSLTESMMTVAQRELFQLTQEADWCYQSEYGRF
RVHRFVQSRGYAAVMRYIPSDIPTLQSLESPDILADICRYRQGLVLVTGATGSGKSTTLAAMVDAINRRDEKHIVTIEDP
VEFVHHSQQSLVHHREIGRDSASFAEALRAALRADPDVLLIGELRDPETIRLALTAAETGHLVLATLHTRSAAQSIDRMI
DVFPADEQSRVRTVLAESLQAVIAQRLLPKVGGGRVGCYEVLIATSAVRNLIRENRLPQIESVMQAGAERGMRTMMQDRQ
RLLNLGKALEK

Nucleotide


Download         Length: 996 bp        

>NTDB_id=659188 MKS89_RS13050 WP_072958266.1 2926523..2927518(-) (pilT) [Vibrio gazogenes strain PB1]
ATGGATATATCAACGTTACTGGCTCTTAGTGTAAAACAGAACGCCTCTGATCTACATCTTTCTGCAAATCATCCGCCGAT
GTTACGAATTGATGGTGGATTGAGAAAGGTGGCATGTGAAACTTTATCCAGCCAGACGATCCTGTCTCTGACCGAATCGA
TGATGACCGTCGCACAGCGAGAGCTGTTTCAGCTTACCCAAGAAGCGGACTGGTGTTATCAGAGTGAGTATGGTCGTTTT
CGGGTTCATCGTTTTGTGCAAAGCCGTGGCTATGCTGCGGTAATGCGCTATATTCCGAGTGATATTCCGACGCTCCAATC
CCTTGAATCACCGGATATTCTTGCCGATATTTGTCGTTATCGTCAGGGGTTGGTATTGGTTACCGGGGCGACCGGTTCCG
GCAAATCAACAACCTTAGCTGCAATGGTCGATGCAATTAACCGCCGAGATGAGAAGCATATTGTGACGATTGAAGATCCG
GTTGAGTTTGTCCATCACTCTCAGCAGTCTTTGGTGCATCACCGGGAAATCGGGCGCGATAGTGCGTCTTTTGCCGAGGC
GCTGCGCGCTGCATTACGGGCTGATCCGGATGTGTTGCTGATCGGTGAATTGCGCGATCCGGAAACGATTCGCTTGGCCT
TGACCGCAGCAGAAACCGGACATTTGGTGTTGGCAACACTGCATACCCGTAGTGCTGCACAATCAATTGATCGGATGATT
GATGTGTTTCCCGCAGATGAGCAGAGCCGGGTTCGTACTGTGCTGGCCGAATCGCTACAGGCAGTGATTGCCCAGCGCTT
ATTGCCCAAAGTCGGGGGCGGTCGGGTCGGCTGTTATGAAGTGTTGATAGCGACTTCAGCGGTGCGGAATTTGATCCGGG
AAAATCGACTGCCTCAGATTGAATCGGTCATGCAGGCAGGAGCCGAGCGTGGGATGAGAACCATGATGCAAGATCGGCAG
CGGTTATTGAATCTCGGGAAGGCGCTAGAGAAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1M5A6V5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

56.269

98.792

0.556
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

56.269

98.792

0.556
  pilT Acinetobacter baumannii D1279779

54.434

98.792

0.538
  pilT Acinetobacter baumannii strain A118

54.434

98.792

0.538
  pilT Acinetobacter nosocomialis M2

54.434

98.792

0.538
  pilT Acinetobacter baylyi ADP1

54.434

98.792

0.538
  pilT Neisseria meningitidis 8013

52.439

99.094

0.52
  pilT Legionella pneumophila strain ERS1305867

52.923

98.187

0.52
  pilT Legionella pneumophila strain Lp02

52.923

98.187

0.52
  pilT Pseudomonas stutzeri DSM 10701

54.43

95.468

0.52
  pilT Neisseria gonorrhoeae MS11

52.134

99.094

0.517
  pilT Pseudomonas aeruginosa PAK

53.481

95.468

0.511
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.633

100

0.486
  pilU Vibrio cholerae strain A1552

40.881

96.073

0.393
  pilU Pseudomonas stutzeri DSM 10701

40.752

96.375

0.393
  pilU Acinetobacter baylyi ADP1

39.688

96.677

0.384