Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   LU695_RS03825 Genome accession   NZ_CP092500
Coordinates   776153..777133 (+) Length   326 a.a.
NCBI ID   WP_258314118.1    Uniprot ID   -
Organism   Escherichia coli strain A30     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
ICE 732525..791696 776153..777133 within 0


Gene organization within MGE regions


Location: 732525..791696
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LU695_RS03595 (LU695_03595) - 732576..733273 (+) 698 WP_225103636.1 IS1 family transposase -
  LU695_RS03600 (LU695_03600) - 733357..734025 (+) 669 WP_024220863.1 hypothetical protein -
  LU695_RS03605 (LU695_03605) - 734012..734239 (+) 228 WP_001039069.1 antitoxin VbhA family protein -
  LU695_RS03610 (LU695_03610) - 734445..735830 (-) 1386 WP_258314113.1 hypothetical protein -
  LU695_RS03615 (LU695_03615) - 736313..737497 (+) 1185 WP_023892874.1 tyrosine-type recombinase/integrase -
  LU695_RS03620 (LU695_03620) - 737552..737908 (-) 357 WP_029403623.1 Arm DNA-binding domain-containing protein -
  LU695_RS03625 (LU695_03625) - 737958..739871 (-) 1914 WP_023892872.1 DUF927 domain-containing protein -
  LU695_RS03630 (LU695_03630) - 739957..741267 (-) 1311 WP_031250067.1 tyrosine-type recombinase/integrase -
  LU695_RS03635 (LU695_03635) - 741786..742139 (+) 354 WP_154807335.1 hypothetical protein -
  LU695_RS03640 (LU695_03640) - 742132..742563 (+) 432 WP_223042354.1 hypothetical protein -
  LU695_RS03645 (LU695_03645) - 742611..743039 (+) 429 WP_047603613.1 DUF2787 family protein -
  LU695_RS03650 (LU695_03650) - 743098..743562 (+) 465 WP_000286078.1 lecithin retinol acyltransferase family protein -
  LU695_RS22305 - 743622..743780 (+) 159 WP_224688858.1 DeoR family transcriptional regulator -
  LU695_RS03655 (LU695_03655) - 743855..744241 (+) 387 WP_223229455.1 hypothetical protein -
  LU695_RS03660 (LU695_03660) - 744385..745134 (+) 750 WP_000257250.1 DUF3944 domain-containing protein -
  LU695_RS03665 (LU695_03665) - 745237..745950 (+) 714 WP_032165642.1 helix-turn-helix transcriptional regulator -
  LU695_RS03675 (LU695_03675) - 746721..746996 (+) 276 WP_097764301.1 helix-turn-helix transcriptional regulator -
  LU695_RS03680 (LU695_03680) - 747151..748119 (-) 969 WP_074194686.1 IS5 family transposase -
  LU695_RS03685 (LU695_03685) - 748396..749436 (+) 1041 WP_063121769.1 tetratricopeptide repeat protein -
  LU695_RS03690 (LU695_03690) - 749820..751160 (+) 1341 WP_174580862.1 sensor domain-containing diguanylate cyclase -
  LU695_RS03695 (LU695_03695) - 751527..751688 (-) 162 WP_174580863.1 hypothetical protein -
  LU695_RS03700 (LU695_03700) - 751841..753379 (-) 1539 WP_087896599.1 IS66-like element ISEc22 family transposase -
  LU695_RS03705 (LU695_03705) tnpB 753428..753775 (-) 348 WP_000612626.1 IS66 family insertion sequence element accessory protein TnpB -
  LU695_RS03710 (LU695_03710) tnpA 753772..754152 (-) 381 WP_001466366.1 IS66-like element accessory protein TnpA -
  LU695_RS03715 (LU695_03715) - 754228..754638 (-) 411 WP_250907922.1 EcsC family protein -
  LU695_RS03720 (LU695_03720) - 754989..755342 (-) 354 WP_047627235.1 hypothetical protein -
  LU695_RS03725 (LU695_03725) - 756018..756319 (+) 302 Protein_731 integrase core domain-containing protein -
  LU695_RS03730 (LU695_03730) - 756750..758042 (+) 1293 WP_029490416.1 MFS transporter -
  LU695_RS03735 (LU695_03735) - 758503..760077 (-) 1575 WP_404853396.1 phosphoethanolamine transferase -
  LU695_RS03740 (LU695_03740) - 760079..761059 (+) 981 WP_258314114.1 IS5-like element ISKpn26 family transposase -
  LU695_RS03745 (LU695_03745) - 761088..761785 (+) 698 WP_095033700.1 IS1-like element IS1B family transposase -
  LU695_RS03750 (LU695_03750) - 761802..761918 (-) 117 Protein_736 hypothetical protein -
  LU695_RS03755 (LU695_03755) glcB 762274..764445 (-) 2172 WP_258314115.1 malate synthase G -
  LU695_RS03760 (LU695_03760) glcG 764467..764871 (-) 405 WP_000853256.1 GlcG family protein -
  LU695_RS03765 (LU695_03765) glcF 764876..766099 (-) 1224 WP_001194666.1 glycolate oxidase subunit GlcF -
  LU695_RS03770 (LU695_03770) glcE 766110..767162 (-) 1053 WP_258314116.1 glycolate oxidase subunit GlcE -
  LU695_RS03775 (LU695_03775) glcD 767162..768661 (-) 1500 WP_258314117.1 glycolate oxidase subunit GlcD -
  LU695_RS03780 (LU695_03780) glcC 768912..769676 (+) 765 WP_001297764.1 transcriptional regulator GlcC -
  LU695_RS03785 (LU695_03785) yghO 769683..770825 (-) 1143 WP_000388786.1 protein YghO -
  LU695_RS03800 (LU695_03800) hemW 772830..773966 (-) 1137 WP_000239917.1 radical SAM family heme chaperone HemW -
  LU695_RS03805 (LU695_03805) rdgB 773959..774552 (-) 594 WP_001174735.1 XTP/dITP diphosphatase -
  LU695_RS03810 (LU695_03810) yggU 774560..774850 (-) 291 WP_001277222.1 DUF167 family protein YggU -
  LU695_RS03815 (LU695_03815) yggT 774847..775413 (-) 567 WP_001094831.1 osmotic shock tolerance protein YggT -
  LU695_RS03820 (LU695_03820) yggS 775431..776135 (-) 705 WP_000997795.1 pyridoxal phosphate homeostasis protein -
  LU695_RS03825 (LU695_03825) pilT 776153..777133 (+) 981 WP_258314118.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  LU695_RS03830 (LU695_03830) ruvX 777322..777738 (-) 417 WP_000017111.1 Holliday junction resolvase RuvX -
  LU695_RS03835 (LU695_03835) yqgE 777738..778301 (-) 564 WP_001053178.1 YqgE/AlgH family protein -
  LU695_RS03840 (LU695_03840) gshB 778410..779360 (-) 951 WP_000593273.1 glutathione synthase -
  LU695_RS03845 (LU695_03845) rsmE 779373..780104 (-) 732 WP_001222508.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  LU695_RS03850 (LU695_03850) endA 780184..780891 (-) 708 WP_000286500.1 deoxyribonuclease I -
  LU695_RS03855 (LU695_03855) yggI 780986..781483 (-) 498 WP_000858396.1 SprT family zinc-dependent metalloprotease -
  LU695_RS03860 (LU695_03860) galP 781560..782954 (-) 1395 WP_016242975.1 galactose/proton symporter -
  LU695_RS03865 (LU695_03865) metK 783390..784544 (-) 1155 WP_001062128.1 methionine adenosyltransferase -
  LU695_RS03870 (LU695_03870) yqgD 784600..784851 (+) 252 WP_001303651.1 protein YqgD -
  LU695_RS22155 yqgG 784962..785105 (+) 144 WP_001297404.1 protein YqgG -
  LU695_RS03875 (LU695_03875) yqgB 785199..785330 (+) 132 WP_001297406.1 acid stress response protein YqgB -
  LU695_RS03880 (LU695_03880) speA 785339..787315 (+) 1977 WP_001295380.1 biosynthetic arginine decarboxylase -
  LU695_RS03885 (LU695_03885) speB 787451..788371 (+) 921 WP_000105566.1 agmatinase -
  LU695_RS03890 (LU695_03890) loiP 788577..789335 (-) 759 WP_000701842.1 metalloprotease LoiP -
  LU695_RS03895 (LU695_03895) tkt 789613..791604 (+) 1992 WP_000098614.1 transketolase -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 36013.19 Da        Isoelectric Point: 5.9975

>NTDB_id=658222 LU695_RS03825 WP_258314118.1 776153..777133(+) (pilT) [Escherichia coli strain A30]
MNIEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDVEELLREWLDDDQRAILLENGQLDFAVSLAENQRLRG
SAFAQRQGISLALRLLPSHCPQLEQLGAPTVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YIYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETRHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVGLFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQQR
VGEGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=658222 LU695_RS03825 WP_258314118.1 776153..777133(+) (pilT) [Escherichia coli strain A30]
ATGAATATAGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGCATTCGTGGCAGAATGGAAGCTGCGCCGTTTGATGCGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGGCAATATTGCTGGAAAATGGCCAGCTGGATTTTGCCGTGTCGCTGGCGGAAAACCAGCGGTTGCGTGGC
AGTGCGTTCGCGCAACGGCAAGGTATTTCTCTGGCATTACGGTTGTTACCTTCGCACTGTCCACAGCTCGAACAGCTTGG
CGCACCAACGGTATTGCCGGAATTACTCAAGAGCGAGAATGGCCTGATTCTGGTGACGGGGGCGACGGGGAGCGGCAAAT
CTACCACGCTGGCGGCGATGGTTGGCTATCTCAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTTGAA
TATATCTATGCCAGCCAGCGATGTTTGATCCAGCAGCGGGAAATTGGTTTGCACTGTATGACGTTCGCATCGGGATTGCG
GGCCGCATTGCGGGAAGATCCTGATGTGATTTTGCTCGGAGAGCTGCGTGACAGCGAGACAATCCGTCTGGCACTGACGG
CGGCAGAAACCAGGCATTTGGTGCTGGCAACATTACATACGCGTGGTGCCGCGCAGGCAGTTGAGCGACTGGTGGATTCA
TTTCCGGCGCAGGAAAAAGATCCCGTACGTAATCAACTGGCAGGTAGTTTACGGGCCGTGTTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGGGCTGTTTGAATTATTGATTAACACTCCCGCGGTGGGGAATTTGATTCGCGAAGGGA
AAACCCACCAGTTACCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGATAACGTTTCAGCAGAGTTATCAGCAGCGG
GTGGGGGAAGGGCGTTTGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(62-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.235

100

0.494
  pilT Vibrio cholerae strain A1552

49.235

100

0.494
  pilT Neisseria meningitidis 8013

48.476

100

0.488
  pilT Neisseria gonorrhoeae MS11

48.171

100

0.485
  pilT Acinetobacter baylyi ADP1

46.483

100

0.466
  pilT Acinetobacter baumannii D1279779

46.177

100

0.463
  pilT Acinetobacter nosocomialis M2

46.177

100

0.463
  pilT Acinetobacter baumannii strain A118

46.177

100

0.463
  pilT Pseudomonas stutzeri DSM 10701

46.177

100

0.463
  pilT Pseudomonas aeruginosa PAK

45.872

100

0.46
  pilT Legionella pneumophila strain ERS1305867

44.648

100

0.448
  pilT Legionella pneumophila strain Lp02

44.648

100

0.448
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.138

97.546

0.411
  pilU Vibrio cholerae strain A1552

39.514

100

0.399
  pilU Pseudomonas stutzeri DSM 10701

37.273

100

0.377
  pilB Legionella pneumophila strain ERS1305867

30.89

100

0.362