Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   MJ595_RS06095 Genome accession   NZ_CP092489
Coordinates   1400787..1401824 (+) Length   345 a.a.
NCBI ID   WP_263322468.1    Uniprot ID   -
Organism   Endozoicomonas sp. Mp262     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1395787..1406824
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MJ595_RS06070 - 1396848..1397486 (+) 639 WP_263081551.1 hypothetical protein -
  MJ595_RS06075 - 1397467..1398405 (+) 939 WP_263081552.1 alpha/beta hydrolase -
  MJ595_RS06080 - 1398479..1399033 (-) 555 WP_263081553.1 YggT family protein -
  MJ595_RS06085 proC 1399085..1399912 (-) 828 WP_263081554.1 pyrroline-5-carboxylate reductase -
  MJ595_RS06090 - 1399927..1400619 (-) 693 WP_263081555.1 YggS family pyridoxal phosphate-dependent enzyme -
  MJ595_RS06095 pilT 1400787..1401824 (+) 1038 WP_263322468.1 type IV pilus twitching motility protein PilT Machinery gene
  MJ595_RS06100 ruvX 1401817..1402254 (-) 438 WP_263081556.1 Holliday junction resolvase RuvX -
  MJ595_RS06105 - 1402325..1402897 (-) 573 WP_263081557.1 YqgE/AlgH family protein -
  MJ595_RS06110 - 1403051..1403530 (-) 480 WP_263081558.1 helix-turn-helix domain-containing protein -
  MJ595_RS06115 - 1403527..1403868 (-) 342 WP_263081559.1 DUF4258 domain-containing protein -
  MJ595_RS06120 - 1404006..1404647 (-) 642 WP_263081560.1 hypothetical protein -
  MJ595_RS06125 - 1405051..1405932 (-) 882 WP_263081561.1 energy transducer TonB -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38172.00 Da        Isoelectric Point: 6.5943

>NTDB_id=658046 MJ595_RS06095 WP_263322468.1 1400787..1401824(+) (pilT) [Endozoicomonas sp. Mp262]
MDITELLAFSNKQGASDLHLSAGMPPMIRIDGDVRRVNVPAMDHKEVHGLIYDIMSDKQRKDYEELLEVDFSFEVPGIAR
FRVNAFNQSRGAGAVFRTIPTKILSMEDLGMGQVFRDISVKPRGLAVVTGPTGSGKSTTLAAMLDYINDTKYEHILTIED
PIEFVHPPKKCLVNQREVQRDTHGFNEALRSALREDPDIILVGEMRDLETIRLALTAAETGHMVFGTLHTTSAAKTVDRI
IDVFPADEKAMVRAMLSESLEAVISQTLLKKKGGGRVAAHEIMLGTSAIRNLIREGKIAQMYSSIQTGQALGMQTLDQCL
EKLIKQGLITPEAARDKAKDPDKFR

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=658046 MJ595_RS06095 WP_263322468.1 1400787..1401824(+) (pilT) [Endozoicomonas sp. Mp262]
ATGGATATTACTGAACTGCTTGCATTTAGCAACAAACAAGGAGCATCGGATCTGCATCTATCTGCCGGTATGCCTCCTAT
GATCAGGATTGATGGCGATGTCAGGCGAGTGAATGTGCCGGCGATGGATCATAAGGAAGTGCACGGGCTTATTTACGATA
TTATGAGCGACAAGCAGCGTAAGGACTATGAAGAGCTTCTAGAAGTGGACTTTTCCTTTGAAGTGCCGGGAATAGCCCGT
TTTCGTGTTAATGCTTTCAATCAGTCCAGAGGGGCTGGCGCTGTTTTCAGAACCATTCCCACCAAGATTTTATCCATGGA
AGATTTGGGTATGGGGCAAGTGTTCAGGGATATTTCGGTAAAACCGAGGGGCCTGGCTGTTGTGACGGGACCTACCGGCT
CGGGAAAGAGTACCACCCTGGCCGCGATGCTGGACTACATTAACGACACCAAATATGAACATATACTCACCATCGAGGAC
CCCATTGAATTTGTTCATCCCCCTAAAAAATGCCTGGTTAACCAGCGGGAAGTACAAAGGGATACCCATGGCTTTAATGA
GGCCCTTCGTTCAGCCCTGCGGGAAGACCCTGATATTATCCTGGTGGGTGAAATGCGGGATCTTGAAACCATCCGGCTGG
CATTAACGGCGGCGGAAACCGGTCATATGGTCTTCGGAACCCTTCATACCACATCGGCAGCAAAAACCGTTGACCGGATT
ATTGATGTATTCCCTGCCGATGAGAAGGCCATGGTTCGGGCCATGCTGTCAGAATCCCTGGAAGCGGTTATTTCCCAGAC
ACTGCTCAAGAAAAAAGGAGGTGGCCGGGTAGCAGCCCATGAGATTATGCTGGGAACCTCTGCTATCCGAAACCTGATCC
GTGAAGGCAAGATTGCCCAGATGTACTCATCGATTCAGACCGGCCAGGCTCTGGGCATGCAAACCCTGGATCAATGTCTC
GAAAAGTTGATCAAGCAAGGGTTAATCACACCAGAGGCCGCCCGGGATAAAGCCAAAGATCCTGACAAGTTTAGGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

81.977

99.71

0.817
  pilT Pseudomonas aeruginosa PAK

80.814

99.71

0.806
  pilT Acinetobacter nosocomialis M2

79.36

99.71

0.791
  pilT Acinetobacter baumannii D1279779

79.36

99.71

0.791
  pilT Acinetobacter baumannii strain A118

79.36

99.71

0.791
  pilT Acinetobacter baylyi ADP1

78.779

99.71

0.786
  pilT Legionella pneumophila strain Lp02

71.221

99.71

0.71
  pilT Legionella pneumophila strain ERS1305867

71.221

99.71

0.71
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.953

97.681

0.664
  pilT Vibrio cholerae strain A1552

67.953

97.681

0.664
  pilT Neisseria gonorrhoeae MS11

65.507

100

0.655
  pilT Neisseria meningitidis 8013

65.507

100

0.655
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.725

100

0.507
  pilU Pseudomonas stutzeri DSM 10701

41.429

100

0.42
  pilU Acinetobacter baylyi ADP1

40.058

100

0.403
  pilU Vibrio cholerae strain A1552

39.017

100

0.391