Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   MJ598_RS09195 Genome accession   NZ_CP092481
Coordinates   2071555..2072607 (-) Length   350 a.a.
NCBI ID   WP_240259155.1    Uniprot ID   -
Organism   Clostridium perfringens strain Qinghai-1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2066555..2077607
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MJ598_RS09175 sigE 2067149..2067856 (-) 708 WP_003449468.1 RNA polymerase sporulation sigma factor SigE -
  MJ598_RS09180 spoIIGA 2067870..2068676 (-) 807 WP_096516432.1 sigma-E processing peptidase SpoIIGA -
  MJ598_RS09185 ftsZ 2069080..2070225 (-) 1146 WP_096516434.1 cell division protein FtsZ -
  MJ598_RS09190 - 2070239..2071477 (-) 1239 WP_096516435.1 cell division FtsA domain-containing protein -
  MJ598_RS09195 pilT 2071555..2072607 (-) 1053 WP_240259155.1 type IV pilus twitching motility protein PilT Machinery gene
  MJ598_RS09200 sigK 2072788..2073489 (-) 702 WP_240259156.1 RNA polymerase sporulation sigma factor SigK -
  MJ598_RS09205 - 2073556..2075220 (-) 1665 WP_240259157.1 penicillin-binding transpeptidase domain-containing protein -
  MJ598_RS09210 udk 2075338..2075964 (-) 627 WP_003459577.1 uridine kinase -
  MJ598_RS09215 - 2075964..2077193 (-) 1230 WP_003459619.1 U32 family peptidase -

Sequence

Protein


Download         Length: 350 a.a.        Molecular weight: 39020.68 Da        Isoelectric Point: 5.4454

>NTDB_id=657971 MJ598_RS09195 WP_240259155.1 2071555..2072607(-) (pilT) [Clostridium perfringens strain Qinghai-1]
MQSLAELLELTVKEGASDLHLTVGISPIIKVNGKLVRLEHEILRPEDTEAYAREILQDAYEKYDVIGEYDTSYSIHGKGR
FRVNIYKQRNSTALAIRVISLDMPTLDSLGYPDTLKDICNLKRGLVLVTGPTGSGKSTTLAALINEINSNRESHIITIED
PIEFLHKHNKSIVNQREIGKDTLSYERALKAALREDPDVILIGEMRDLETISTAITAAETGHLVFSTLHTIGAAKTIDRI
VDVFPPHQQEQIKIQLASVLQTIISQQLVETVDGDRTAALEIMVATPAIKNLIREGKTHQIESSIQTGSKYGMRTMDMEL
SNLYREGVITQETAMNSAIDREILSRLLMY

Nucleotide


Download         Length: 1053 bp        

>NTDB_id=657971 MJ598_RS09195 WP_240259155.1 2071555..2072607(-) (pilT) [Clostridium perfringens strain Qinghai-1]
ATGCAAAGTTTAGCAGAACTTTTAGAGTTAACAGTTAAAGAAGGAGCATCTGATTTGCATTTAACAGTAGGTATTTCTCC
TATAATAAAAGTTAATGGAAAATTAGTAAGGTTAGAACATGAAATATTACGCCCTGAAGATACTGAAGCGTATGCTAGAG
AGATATTACAGGATGCATATGAAAAGTATGATGTTATTGGAGAATACGATACATCATATTCAATACATGGTAAAGGTAGA
TTTAGAGTTAATATATATAAACAAAGAAATAGTACAGCTTTAGCTATAAGGGTTATATCTTTAGATATGCCAACTTTAGA
TAGCTTAGGATATCCAGATACTTTAAAGGATATATGCAATCTTAAAAGAGGTCTTGTATTAGTTACAGGTCCTACTGGAA
GTGGTAAAAGTACAACTTTAGCTGCTTTAATAAATGAAATAAATAGTAATAGAGAATCTCATATAATAACAATAGAGGAT
CCTATAGAGTTTCTTCATAAACATAATAAATCTATTGTAAACCAAAGAGAGATCGGTAAGGATACTTTAAGTTATGAAAG
AGCTTTAAAAGCAGCTCTTAGAGAAGATCCAGATGTTATTTTAATTGGAGAAATGAGAGATTTAGAGACTATTTCAACAG
CTATAACAGCGGCAGAGACTGGACATTTAGTATTTTCAACTCTTCACACCATAGGAGCTGCTAAGACTATAGATAGAATA
GTGGATGTTTTTCCACCACATCAACAGGAGCAAATAAAAATACAATTAGCTTCTGTTTTACAAACAATTATATCACAGCA
ATTAGTAGAAACAGTTGATGGAGATAGAACTGCCGCATTAGAAATAATGGTTGCTACACCAGCTATAAAAAATTTAATAA
GGGAAGGAAAAACTCATCAAATAGAATCATCCATACAAACAGGAAGTAAATATGGAATGAGAACAATGGATATGGAACTT
TCAAATCTTTACAGAGAAGGTGTAATAACACAAGAAACTGCAATGAACTCTGCTATAGATAGGGAAATTCTAAGTCGATT
ATTGATGTATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(79-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.422

98.857

0.489
  pilT Neisseria gonorrhoeae MS11

49.709

98.286

0.489
  pilT Neisseria meningitidis 8013

49.709

98.286

0.489
  pilT Legionella pneumophila strain ERS1305867

49.123

97.714

0.48
  pilT Legionella pneumophila strain Lp02

49.123

97.714

0.48
  pilT Pseudomonas aeruginosa PAK

50

96

0.48
  pilT Acinetobacter baumannii strain A118

50

96

0.48
  pilT Acinetobacter baumannii D1279779

50

96

0.48
  pilT Acinetobacter nosocomialis M2

50

96

0.48
  pilT Acinetobacter baylyi ADP1

50.301

94.857

0.477
  pilT Pseudomonas stutzeri DSM 10701

49.702

96

0.477
  pilT Vibrio cholerae strain A1552

50.765

93.429

0.474
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.765

93.429

0.474
  pilU Pseudomonas stutzeri DSM 10701

44.277

94.857

0.42
  pilU Vibrio cholerae strain A1552

40.356

96.286

0.389
  pilU Acinetobacter baylyi ADP1

39.823

96.857

0.386