Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   MJ023_RS21070 Genome accession   NZ_CP092461
Coordinates   4362199..4363221 (-) Length   340 a.a.
NCBI ID   WP_060445093.1    Uniprot ID   -
Organism   Serratia marcescens strain RH10     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4357199..4368221
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MJ023_RS21040 (MJ023_004175) - 4358000..4358512 (+) 513 WP_033639394.1 SprT family zinc-dependent metalloprotease -
  MJ023_RS21045 (MJ023_004176) endA 4358614..4359309 (+) 696 WP_015379043.1 deoxyribonuclease I -
  MJ023_RS21050 (MJ023_004177) rsmE 4359379..4360110 (+) 732 WP_004937458.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  MJ023_RS21055 (MJ023_004178) gshB 4360121..4361071 (+) 951 WP_016930084.1 glutathione synthase -
  MJ023_RS21060 (MJ023_004179) - 4361217..4361780 (+) 564 WP_033635940.1 YqgE/AlgH family protein -
  MJ023_RS21065 (MJ023_004180) ruvX 4361780..4362202 (+) 423 WP_025304246.1 Holliday junction resolvase RuvX -
  MJ023_RS21070 (MJ023_004181) pilT 4362199..4363221 (-) 1023 WP_060445093.1 type IV pilus twitching motility protein PilT Machinery gene
  MJ023_RS21075 (MJ023_004182) - 4363242..4363949 (+) 708 WP_115116486.1 YggS family pyridoxal phosphate-dependent enzyme -
  MJ023_RS21080 (MJ023_004183) proC 4363969..4364790 (+) 822 WP_048796772.1 pyrroline-5-carboxylate reductase -
  MJ023_RS21085 (MJ023_004184) - 4364821..4365375 (+) 555 WP_004937436.1 YggT family protein -
  MJ023_RS21090 (MJ023_004185) yggU 4365372..4365662 (+) 291 WP_004948014.1 DUF167 family protein YggU -
  MJ023_RS21095 (MJ023_004186) - 4365681..4366274 (+) 594 WP_243810605.1 XTP/dITP diphosphatase -
  MJ023_RS21100 (MJ023_004187) hemW 4366267..4367409 (+) 1143 WP_033635947.1 radical SAM family heme chaperone HemW -
  MJ023_RS21105 (MJ023_004188) - 4367447..4367881 (-) 435 WP_016930094.1 DUF29 domain-containing protein -

Sequence

Protein


Download         Length: 340 a.a.        Molecular weight: 36488.78 Da        Isoelectric Point: 7.2889

>NTDB_id=657799 MJ023_RS21070 WP_060445093.1 4362199..4363221(-) (pilT) [Serratia marcescens strain RH10]
MDIDEFVALSVKHNASDLHLCAGHPPMLRIDGELQPLAAAPTLTAQGVQALCDGLLNAQQRESLRRLGQVDLALHRPGGE
RLRANVFQQSAGISLALRRIAGQSPSLAELAAPAIVPALLRRDDGLILVTGATGSGKSTTLAAMIDEINRHQQRHILTLE
DPIEFLHRSRRSLIQQREIGRDSHSFDAALRAALREDPDVILLGELRDTATIRLALTAAETGHLVLATLHTRSAPQAVER
LVDVFPAEEKPYVRAQLAGSLQAVIAQKLMRRPGGGRVAIFEVLTATAAVSNLIREGKTHQLASVLQTGAQSGMQTFEQG
LQQRIDAGVLGECAGERAGV

Nucleotide


Download         Length: 1023 bp        

>NTDB_id=657799 MJ023_RS21070 WP_060445093.1 4362199..4363221(-) (pilT) [Serratia marcescens strain RH10]
ATGGATATCGATGAATTCGTGGCCCTTAGTGTAAAGCATAATGCTTCCGATCTGCACCTTTGTGCCGGTCATCCGCCGAT
GTTGCGCATCGACGGTGAACTGCAGCCGCTGGCGGCGGCGCCAACGCTGACGGCGCAAGGCGTGCAGGCCCTCTGCGACG
GATTGCTGAATGCGCAGCAGCGCGAGAGCCTGCGGCGGCTGGGGCAGGTCGATCTGGCGTTGCACAGGCCGGGCGGGGAG
CGGCTGCGGGCCAACGTTTTTCAACAAAGCGCGGGGATTTCTCTCGCGTTGCGGCGTATCGCCGGGCAATCGCCTTCGCT
TGCCGAGCTGGCGGCGCCGGCCATTGTCCCGGCGTTGCTGCGGCGCGACGACGGGCTTATCCTGGTCACCGGCGCCACCG
GCAGCGGCAAGTCCACCACGTTGGCGGCGATGATCGACGAGATCAACCGGCACCAGCAGCGGCATATTTTGACGCTGGAG
GATCCGATCGAATTCCTGCACCGCAGCCGGCGATCGCTGATCCAGCAGCGGGAGATCGGCCGCGACAGCCACAGCTTCGA
TGCGGCGTTGCGCGCCGCGCTGCGCGAAGATCCGGATGTGATCCTGCTGGGCGAGCTGCGCGATACCGCCACCATCCGGC
TGGCGCTCACTGCGGCGGAGACCGGCCACCTGGTGCTGGCGACGCTGCATACCCGCAGCGCGCCGCAGGCGGTGGAACGG
CTGGTGGACGTGTTTCCGGCGGAAGAGAAACCCTATGTGCGCGCCCAGCTGGCCGGCAGTCTGCAGGCGGTGATCGCGCA
AAAGCTGATGAGGCGCCCCGGCGGCGGACGCGTGGCGATCTTTGAAGTGCTGACGGCGACGGCGGCGGTCAGCAACCTGA
TCCGCGAAGGGAAAACGCATCAGTTGGCGAGCGTGTTGCAAACCGGCGCACAGTCCGGCATGCAAACCTTTGAGCAAGGT
TTGCAGCAGCGGATCGACGCCGGCGTGTTGGGGGAGTGCGCGGGGGAAAGGGCGGGCGTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

54.848

97.059

0.532
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

54.848

97.059

0.532
  pilT Neisseria meningitidis 8013

50.294

100

0.503
  pilT Acinetobacter baylyi ADP1

51.36

97.353

0.5
  pilT Pseudomonas aeruginosa PAK

49.267

100

0.494
  pilT Acinetobacter nosocomialis M2

50.909

97.059

0.494
  pilT Acinetobacter baumannii strain A118

50.909

97.059

0.494
  pilT Acinetobacter baumannii D1279779

50.909

97.059

0.494
  pilT Neisseria gonorrhoeae MS11

49.704

99.412

0.494
  pilT Pseudomonas stutzeri DSM 10701

48.673

99.706

0.485
  pilT Legionella pneumophila strain Lp02

48.529

100

0.485
  pilT Legionella pneumophila strain ERS1305867

48.529

100

0.485
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.029

100

0.453
  pilU Pseudomonas stutzeri DSM 10701

38.643

99.706

0.385
  pilU Acinetobacter baylyi ADP1

40.248

95

0.382
  pilU Vibrio cholerae strain A1552

39.877

95.882

0.382