Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   MJO48_RS18115 Genome accession   NZ_CP092458
Coordinates   4122919..4123932 (-) Length   337 a.a.
NCBI ID   WP_239787823.1    Uniprot ID   -
Organism   Dickeya fangzhongdai strain 643b     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4117919..4128932
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MJO48_RS18090 (MJO48_18090) - 4118131..4119717 (+) 1587 WP_038920105.1 methyl-accepting chemotaxis protein -
  MJO48_RS18095 (MJO48_18095) - 4120024..4120935 (+) 912 WP_239787813.1 PucR family transcriptional regulator -
  MJO48_RS18100 (MJO48_18100) codA 4120938..4122188 (+) 1251 WP_239787820.1 cytosine deaminase -
  MJO48_RS18105 (MJO48_18105) - 4122345..4122548 (+) 204 WP_239787822.1 hypothetical protein -
  MJO48_RS18110 (MJO48_18110) - 4122583..4122774 (-) 192 WP_239789221.1 hypothetical protein -
  MJO48_RS18115 (MJO48_18115) pilT 4122919..4123932 (-) 1014 WP_239787823.1 type IV pilus twitching motility protein PilT Machinery gene
  MJO48_RS18120 (MJO48_18120) - 4123960..4124673 (+) 714 WP_107760887.1 YggS family pyridoxal phosphate-dependent enzyme -
  MJO48_RS18125 (MJO48_18125) proC 4124707..4125528 (+) 822 WP_239787832.1 pyrroline-5-carboxylate reductase -
  MJO48_RS18130 (MJO48_18130) - 4125572..4126126 (+) 555 WP_038920114.1 YggT family protein -
  MJO48_RS18135 (MJO48_18135) yggU 4126123..4126413 (+) 291 WP_038920115.1 DUF167 family protein YggU -
  MJO48_RS18140 (MJO48_18140) - 4126496..4127089 (+) 594 WP_038920116.1 XTP/dITP diphosphatase -
  MJO48_RS18145 (MJO48_18145) hemW 4127082..4128221 (+) 1140 WP_049853840.1 radical SAM family heme chaperone HemW -
  MJO48_RS18150 (MJO48_18150) - 4128353..4128481 (+) 129 WP_038666077.1 entericidin A/B family lipoprotein -

Sequence

Protein


Download         Length: 337 a.a.        Molecular weight: 36834.01 Da        Isoelectric Point: 6.7155

>NTDB_id=657753 MJO48_RS18115 WP_239787823.1 4122919..4123932(-) (pilT) [Dickeya fangzhongdai strain 643b]
MDVSEWVRSSVKQNVSDLHLCSGHPPVWRIDGQLVADAAQPPLHNDELEQWCGSWLEPACEQQLRQSGQTDCALTLPEGQ
RLRANLFMQRHGLSAALRLIPSHQPDLQALGAPPVVAELADRPDGLILVTGATGSGKSTTLAAMIASLNQRRARHIITLE
DPIEFLHVSQRCLIQQREIGRHSDAFASALRAALREDPDVLLLGELRDSETIRLALTAAETGHLVLATLHTRRAVQSVDR
LVDVFPAEEKAFVRTQLAASLQAVVAQKLLPGAQGGRVALFEVLLATPAVSNLIREGKTHQLPSLLQTGGQVGMQTFEQS
YQQRCREGRLRSEMNAG

Nucleotide


Download         Length: 1014 bp        

>NTDB_id=657753 MJO48_RS18115 WP_239787823.1 4122919..4123932(-) (pilT) [Dickeya fangzhongdai strain 643b]
ATGGATGTGAGTGAATGGGTGCGGTCTAGTGTAAAACAGAATGTGTCCGATCTGCACCTGTGCAGCGGTCATCCGCCGGT
TTGGCGTATCGACGGGCAACTGGTGGCGGATGCTGCGCAGCCGCCGTTGCACAACGACGAGCTGGAGCAGTGGTGCGGCA
GCTGGCTGGAACCAGCCTGCGAGCAGCAACTGCGGCAAAGCGGGCAGACCGACTGCGCGCTGACGCTGCCGGAGGGGCAG
CGGTTACGGGCCAATCTGTTTATGCAGCGCCACGGCCTGTCGGCGGCGCTGCGCCTGATCCCGTCACATCAGCCGGATCT
ACAGGCGTTGGGCGCGCCGCCGGTGGTAGCGGAGCTGGCCGATCGTCCCGATGGTCTGATTCTGGTGACCGGCGCAACCG
GCAGCGGTAAATCCACCACGCTGGCGGCGATGATCGCCTCGCTCAATCAACGCCGTGCGCGCCACATTATTACGCTGGAA
GACCCGATTGAGTTCCTGCATGTCAGCCAACGCTGTCTGATTCAACAGCGGGAGATTGGCCGTCATAGCGATGCGTTCGC
CAGTGCGCTGCGGGCGGCGCTGCGCGAAGACCCGGACGTGCTGCTGCTCGGCGAGTTGCGCGACAGCGAGACGATTCGGC
TGGCGCTGACGGCGGCGGAAACCGGTCATCTGGTGTTGGCGACGCTGCATACCCGGCGGGCGGTGCAGTCGGTGGACCGG
CTGGTGGATGTCTTTCCCGCCGAGGAGAAAGCGTTTGTTCGCACGCAACTGGCCGCCAGTCTGCAGGCGGTGGTGGCGCA
GAAACTGTTGCCGGGCGCGCAAGGCGGGCGCGTGGCGCTGTTTGAAGTACTGCTCGCCACCCCGGCGGTCAGCAACCTGA
TCCGCGAAGGTAAAACCCACCAGTTGCCGAGCCTGCTGCAAACCGGCGGCCAGGTCGGCATGCAGACCTTTGAACAGAGT
TACCAGCAGCGATGCCGGGAGGGGAGGCTGCGGTCTGAAATGAATGCCGGTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(62-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.305

97.329

0.49
  pilT Vibrio cholerae strain A1552

50.305

97.329

0.49
  pilT Neisseria meningitidis 8013

48.657

99.407

0.484
  pilT Pseudomonas stutzeri DSM 10701

49.394

97.923

0.484
  pilT Pseudomonas aeruginosa PAK

48.649

98.813

0.481
  pilT Neisseria gonorrhoeae MS11

48.503

99.11

0.481
  pilT Acinetobacter baylyi ADP1

49.085

97.329

0.478
  pilT Acinetobacter nosocomialis M2

48.171

97.329

0.469
  pilT Legionella pneumophila strain Lp02

49.53

94.659

0.469
  pilT Legionella pneumophila strain ERS1305867

49.53

94.659

0.469
  pilT Acinetobacter baumannii D1279779

48.758

95.549

0.466
  pilT Acinetobacter baumannii strain A118

48.758

95.549

0.466
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.768

94.659

0.433
  pilU Vibrio cholerae strain A1552

37.982

100

0.38
  pilU Pseudomonas stutzeri DSM 10701

37.725

99.11

0.374
  pilU Acinetobacter baylyi ADP1

38.199

95.549

0.365