Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   PPC_RS28880 Genome accession   NZ_AP014522
Coordinates   6401495..6402529 (-) Length   344 a.a.
NCBI ID   WP_041120249.1    Uniprot ID   -
Organism   Pseudomonas protegens Cab57     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6396495..6407529
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PPC_RS28850 (PPC_5778) ruvX 6396638..6397075 (+) 438 WP_011064005.1 Holliday junction resolvase RuvX -
  PPC_RS28855 (PPC_5779) pyrR 6397191..6397697 (+) 507 WP_011064006.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  PPC_RS28860 (PPC_5780) - 6397723..6398727 (+) 1005 WP_011064007.1 aspartate carbamoyltransferase catalytic subunit -
  PPC_RS28865 (PPC_5781) - 6398724..6399995 (+) 1272 WP_041120247.1 dihydroorotase -
  PPC_RS28870 (PPC_5782) - 6400123..6400542 (-) 420 WP_011064009.1 NINE protein -
  PPC_RS28875 (PPC_5783) - 6400793..6401416 (+) 624 WP_011064010.1 C40 family peptidase -
  PPC_RS28880 (PPC_5784) pilT 6401495..6402529 (-) 1035 WP_041120249.1 type IV pilus twitching motility protein PilT Machinery gene
  PPC_RS28885 (PPC_5785) - 6402720..6403418 (+) 699 WP_041120251.1 YggS family pyridoxal phosphate-dependent enzyme -
  PPC_RS28890 (PPC_5786) proC 6403453..6404271 (+) 819 WP_019095289.1 pyrroline-5-carboxylate reductase -
  PPC_RS28895 (PPC_5787) - 6404282..6404872 (+) 591 WP_011064014.1 YggT family protein -
  PPC_RS28900 (PPC_5788) - 6404887..6405180 (+) 294 WP_041120253.1 DUF167 domain-containing protein -
  PPC_RS28905 (PPC_5789) metX 6405344..6406483 (+) 1140 WP_011064016.1 homoserine O-succinyltransferase MetX -
  PPC_RS28910 (PPC_5790) metW 6406491..6407111 (+) 621 WP_011064017.1 methionine biosynthesis protein MetW -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37426.20 Da        Isoelectric Point: 7.0747

>NTDB_id=65770 PPC_RS28880 WP_041120249.1 6401495..6402529(-) (pilT) [Pseudomonas protegens Cab57]
MDITELLRLSASRGASDLHLSAGLAPMLRIDGDIRPLPGPALEGPQVQALIHELMSEAQRQAFAACNDLDFAYELPGVAR
FRVNAFRHARGVGAVLRLIPARVQSLEELGLGEVFRQITEAPRGLVLVTGPTGSGKSTTLAAMIDHLNRHRQQHILTIED
PIEFVHPQKNCLLHQREVQRDTQSFATALRAALREDPDVILLGELRDLETIRLALTAAETGHLVLATLHTSSAAKTIDRL
VDVFPGEEKALVRSMLAESLQAVVAQVLVKKVGGGRVAAHEIMLGSPAIRNLIREDKVAQMYSAIQTGGAQGMRTLDMSL
KALLGQGLISRDQAREQARMPESF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=65770 PPC_RS28880 WP_041120249.1 6401495..6402529(-) (pilT) [Pseudomonas protegens Cab57]
ATGGACATCACCGAACTCTTGCGCTTGAGCGCTTCCCGGGGCGCTTCGGACCTGCATCTGTCCGCCGGGCTGGCGCCGAT
GTTGCGCATCGATGGCGATATTCGCCCGCTGCCGGGCCCGGCCCTGGAAGGGCCACAGGTGCAGGCGCTGATCCATGAAC
TGATGAGCGAAGCACAGCGCCAGGCGTTCGCCGCCTGCAATGACCTGGATTTCGCCTATGAACTGCCGGGTGTGGCGCGG
TTTCGGGTCAATGCCTTCCGGCACGCCCGTGGCGTCGGTGCGGTGCTGCGCCTGATTCCCGCGCGGGTACAGAGCCTGGA
GGAGCTGGGGCTGGGGGAAGTGTTTCGGCAGATTACCGAGGCGCCCCGCGGGCTGGTGCTGGTGACCGGGCCCACCGGCA
GCGGCAAGTCCACCACACTGGCGGCGATGATCGACCACCTGAACCGGCACCGCCAGCAGCACATCCTGACCATCGAAGAC
CCTATCGAATTCGTGCATCCGCAGAAAAACTGCCTGCTGCACCAGCGCGAAGTGCAGCGCGATACCCAGAGCTTCGCCAC
GGCCTTGCGCGCGGCGCTGCGGGAAGATCCCGACGTGATCCTGCTGGGCGAACTGCGGGACCTGGAAACCATCCGCCTGG
CCCTGACCGCCGCGGAAACCGGGCACCTGGTACTGGCCACCCTGCACACCTCGTCGGCGGCCAAGACCATCGACCGGCTG
GTGGACGTGTTTCCGGGGGAGGAAAAGGCCCTGGTGCGCTCGATGCTGGCCGAGTCGCTGCAGGCAGTGGTTGCGCAAGT
GCTGGTGAAGAAGGTGGGCGGTGGCCGGGTAGCGGCGCACGAGATCATGCTGGGCAGCCCGGCGATCCGTAACCTGATCC
GCGAGGACAAGGTGGCGCAGATGTATTCGGCGATCCAGACGGGCGGCGCCCAGGGCATGCGCACCCTGGACATGAGCCTC
AAGGCATTGCTCGGGCAGGGGTTGATCAGCCGCGATCAGGCGCGGGAGCAGGCGCGGATGCCGGAGAGCTTTTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(13-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

70.64

100

0.706
  pilT Pseudomonas stutzeri DSM 10701

70.64

100

0.706
  pilT Acinetobacter nosocomialis M2

65.988

100

0.66
  pilT Acinetobacter baumannii D1279779

65.988

100

0.66
  pilT Acinetobacter baumannii strain A118

65.988

100

0.66
  pilT Acinetobacter baylyi ADP1

65.988

100

0.66
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

62.647

98.837

0.619
  pilT Vibrio cholerae strain A1552

62.647

98.837

0.619
  pilT Legionella pneumophila strain Lp02

61.538

98.256

0.605
  pilT Legionella pneumophila strain ERS1305867

61.538

98.256

0.605
  pilT Neisseria meningitidis 8013

58.261

100

0.584
  pilT Neisseria gonorrhoeae MS11

57.971

100

0.581
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.754

100

0.529
  pilU Vibrio cholerae strain A1552

41.791

97.384

0.407
  pilU Pseudomonas stutzeri DSM 10701

40.896

97.384

0.398
  pilU Acinetobacter baylyi ADP1

38.551

100

0.387

Multiple sequence alignment