Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   H7R53_RS02055 Genome accession   NZ_AP022087
Coordinates   459552..460229 (-) Length   225 a.a.
NCBI ID   WP_024216246.1    Uniprot ID   -
Organism   Escherichia coli strain WP4-S18-ESBL-07     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 454552..465229
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H7R53_RS02040 (WP4S18E07_04060) chiA 455910..458612 (+) 2703 WP_024216245.1 bifunctional chitinase/lysozyme -
  H7R53_RS02045 (WP4S18E07_04070) bfd 458781..458975 (+) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  H7R53_RS02050 (WP4S18E07_04080) bfr 459047..459523 (+) 477 WP_000675504.1 bacterioferritin -
  H7R53_RS02055 (WP4S18E07_04090) pilD 459552..460229 (-) 678 WP_024216246.1 prepilin peptidase GspO Machinery gene
  H7R53_RS02060 (WP4S18E07_04100) gspM 460229..460690 (-) 462 WP_024216247.1 type II secretion system protein GspM -
  H7R53_RS02065 (WP4S18E07_04110) gspL 460687..461850 (-) 1164 WP_024216248.1 type II secretion system protein GspL -
  H7R53_RS02070 (WP4S18E07_04120) gspK 461865..462848 (-) 984 WP_024216249.1 type II secretion system minor pseudopilin GspK -
  H7R53_RS02075 (WP4S18E07_04130) gspJ 462841..463428 (-) 588 WP_001600564.1 type II secretion system minor pseudopilin GspJ -
  H7R53_RS02080 (WP4S18E07_04140) gspI 463421..463798 (-) 378 WP_001041743.1 type II secretion system minor pseudopilin GspI -
  H7R53_RS02085 (WP4S18E07_04150) gspH 463795..464304 (-) 510 WP_024216250.1 type II secretion system minor pseudopilin GspH -
  H7R53_RS02090 (WP4S18E07_04160) gspG 464312..464749 (-) 438 WP_001202877.1 type II secretion system major pseudopilin GspG -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24855.71 Da        Isoelectric Point: 7.6590

>NTDB_id=65770 H7R53_RS02055 WP_024216246.1 459552..460229(-) (pilD) [Escherichia coli strain WP4-S18-ESBL-07]
MTMLLPLFILVGFMAGYFVNAMAYHLSPLEDKTALTFRQVLIHFRQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFALTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQSGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQHRTITTIAFGPWLALSSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=65770 H7R53_RS02055 WP_024216246.1 459552..460229(-) (pilD) [Escherichia coli strain WP4-S18-ESBL-07]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATGGCAGGTTATTTTGTTAATGCTATGGCCTATCACCTCTC
GCCTTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGATTCATTTCAGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGCGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGCACTCACGCTCAGTGTTATTGATTTTCGCACTCAACTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTCTGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCGTGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCACTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACACCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCAGCAGCA
TGATAAACCTGGGGTATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

33.721

100

0.387

Multiple sequence alignment