Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   MJP36_RS04035 Genome accession   NZ_CP092411
Coordinates   917520..918551 (+) Length   343 a.a.
NCBI ID   WP_244384923.1    Uniprot ID   -
Organism   Pseudomonas palleroniana strain Q1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 912520..923551
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MJP36_RS04005 (MJP36_04005) - 912897..913331 (-) 435 WP_090368220.1 DUF4426 domain-containing protein -
  MJP36_RS04010 (MJP36_04010) metW 913353..913973 (-) 621 WP_060753467.1 methionine biosynthesis protein MetW -
  MJP36_RS04015 (MJP36_04015) - 913981..915120 (-) 1140 WP_090368222.1 homoserine O-succinyltransferase MetX -
  MJP36_RS04020 (MJP36_04020) - 915311..915898 (-) 588 WP_060753465.1 YggT family protein -
  MJP36_RS04025 (MJP36_04025) proC 915924..916742 (-) 819 WP_090368224.1 pyrroline-5-carboxylate reductase -
  MJP36_RS04030 (MJP36_04030) - 916776..917462 (-) 687 WP_244384922.1 YggS family pyridoxal phosphate-dependent enzyme -
  MJP36_RS04035 (MJP36_04035) pilT 917520..918551 (+) 1032 WP_244384923.1 type IV pilus twitching motility protein PilT Machinery gene
  MJP36_RS04040 (MJP36_04040) - 918689..919324 (-) 636 WP_244384924.1 C40 family peptidase -
  MJP36_RS04045 (MJP36_04045) - 919575..920003 (+) 429 WP_060753460.1 NINE protein -
  MJP36_RS04050 (MJP36_04050) - 920135..921406 (-) 1272 WP_104994927.1 dihydroorotase -
  MJP36_RS04055 (MJP36_04055) - 921403..922407 (-) 1005 WP_090368230.1 aspartate carbamoyltransferase catalytic subunit -
  MJP36_RS04060 (MJP36_04060) pyrR 922432..922938 (-) 507 WP_060753457.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  MJP36_RS04065 (MJP36_04065) ruvX 923011..923448 (-) 438 WP_003195067.1 Holliday junction resolvase RuvX -

Sequence

Protein


Download         Length: 343 a.a.        Molecular weight: 37153.90 Da        Isoelectric Point: 7.7478

>NTDB_id=657182 MJP36_RS04035 WP_244384923.1 917520..918551(+) (pilT) [Pseudomonas palleroniana strain Q1]
MDITQLLTASVRRGASDLHLSAGLAPMLRIDGEVWPLEGSILSPPQVADLLSPLLNQHQQKDFETSLETDFAFELPGVAR
FRANVFRHDRGMGAVFRTIAQVRSLESLGLGEVFQRIAQLPRGLVLVTGPTGSGKSTTLAAMIDHLNQHRRQHILTLEDP
IEFIHKPKSALINQRQVHRDTHSFSAALRSALREDPDVILVGELRDLETIRLALTAAETGHLVLGTLHTASAAKTVDRLV
DVFPAGEKAMVRSMLSESLQAVVSQALVKKIGGGRVAAHEIMLGTPAIRNLIREDKGAQMVSAIQTGGALGMKTLDMSLK
ALVSEGLVSREAARELARVPTDI

Nucleotide


Download         Length: 1032 bp        

>NTDB_id=657182 MJP36_RS04035 WP_244384923.1 917520..918551(+) (pilT) [Pseudomonas palleroniana strain Q1]
ATGGATATCACGCAATTGCTCACGGCCAGCGTGCGCCGTGGCGCCTCAGACCTGCATTTGTCCGCCGGTCTGGCGCCGAT
GCTGCGCATCGATGGCGAAGTCTGGCCCCTGGAGGGGTCGATACTTTCGCCACCGCAAGTTGCGGACTTATTGAGCCCTT
TGCTCAATCAACACCAACAAAAGGATTTCGAAACATCTCTTGAAACGGATTTTGCATTTGAATTGCCTGGCGTGGCGCGC
TTTCGGGCCAATGTGTTCCGACACGATCGCGGCATGGGGGCGGTGTTTCGTACCATTGCTCAGGTGCGAAGCCTGGAAAG
CCTGGGCCTCGGGGAGGTGTTCCAGCGTATCGCCCAATTGCCTCGTGGGCTGGTACTGGTGACCGGGCCGACCGGGTCGG
GCAAGTCCACGACACTGGCGGCGATGATCGACCACCTCAACCAGCATCGGCGCCAGCACATCCTCACCCTCGAAGACCCC
ATCGAATTTATCCACAAACCCAAATCGGCGTTGATCAACCAGCGGCAGGTGCACCGTGATACCCACAGCTTCTCGGCGGC
CCTGCGTTCGGCGTTGCGCGAAGACCCGGATGTGATTCTGGTGGGAGAGCTGCGCGACCTGGAAACCATCCGCCTCGCGC
TGACGGCGGCCGAGACCGGGCACCTGGTCCTGGGCACCCTGCACACCGCGTCCGCGGCGAAAACCGTGGACAGGCTGGTG
GATGTATTCCCGGCGGGGGAAAAGGCCATGGTGCGTTCGATGCTGTCGGAGTCTTTGCAGGCCGTCGTGTCCCAGGCGCT
GGTCAAGAAGATCGGCGGCGGGCGGGTAGCGGCTCACGAGATCATGCTGGGCACGCCGGCCATTCGTAACCTGATCCGCG
AGGACAAGGGTGCGCAGATGGTTTCAGCGATCCAGACCGGTGGGGCATTAGGGATGAAGACGTTGGATATGAGCTTGAAG
GCGCTGGTGAGCGAGGGGCTGGTCAGCCGGGAGGCTGCGCGGGAGCTGGCGAGGGTTCCCACAGACATATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(112-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

71.221

100

0.714
  pilT Pseudomonas stutzeri DSM 10701

68.895

100

0.691
  pilT Acinetobacter nosocomialis M2

67.742

99.417

0.673
  pilT Acinetobacter baumannii D1279779

67.449

99.417

0.671
  pilT Acinetobacter baumannii strain A118

67.449

99.417

0.671
  pilT Acinetobacter baylyi ADP1

67.155

99.417

0.668
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

64.438

95.918

0.618
  pilT Vibrio cholerae strain A1552

64.438

95.918

0.618
  pilT Legionella pneumophila strain Lp02

62.13

98.542

0.612
  pilT Legionella pneumophila strain ERS1305867

62.13

98.542

0.612
  pilT Neisseria gonorrhoeae MS11

60.882

99.125

0.603
  pilT Neisseria meningitidis 8013

60.882

99.125

0.603
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.275

100

0.496
  pilU Vibrio cholerae strain A1552

38.841

100

0.391
  pilU Acinetobacter baylyi ADP1

38.15

100

0.385
  pilU Pseudomonas stutzeri DSM 10701

38.806

97.668

0.379