Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   MIS45_RS09055 Genome accession   NZ_CP092361
Coordinates   1853753..1854793 (+) Length   346 a.a.
NCBI ID   WP_249446020.1    Uniprot ID   -
Organism   Wielerella bovis strain CCUG 44465     
Function   pseudopilus biogenesis (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1848753..1859793
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MIS45_RS09020 (MIS45_09020) - 1849236..1849559 (-) 324 WP_249450281.1 hypothetical protein -
  MIS45_RS09025 (MIS45_09025) - 1849646..1850152 (-) 507 WP_249450282.1 GAF domain-containing protein -
  MIS45_RS09030 (MIS45_09030) - 1850221..1851117 (+) 897 WP_249450283.1 beta-1,6-N-acetylglucosaminyltransferase -
  MIS45_RS09035 (MIS45_09035) cyaY 1851204..1851530 (-) 327 WP_249449581.1 iron donor protein CyaY -
  MIS45_RS09040 (MIS45_09040) - 1851610..1851792 (+) 183 WP_249450284.1 lipoprotein -
  MIS45_RS09045 (MIS45_09045) - 1851819..1852193 (+) 375 WP_249450285.1 Imm8 family immunity protein -
  MIS45_RS09050 (MIS45_09050) lysA 1852209..1853429 (+) 1221 WP_249450286.1 diaminopimelate decarboxylase -
  MIS45_RS09055 (MIS45_09055) pilT 1853753..1854793 (+) 1041 WP_249446020.1 type IV pilus twitching motility protein PilT Machinery gene
  MIS45_RS09060 (MIS45_09060) - 1854817..1856142 (+) 1326 WP_249450287.1 PilT/PilU family type 4a pilus ATPase -
  MIS45_RS09065 (MIS45_09065) - 1856408..1857205 (-) 798 WP_249450288.1 S24 family peptidase -
  MIS45_RS09070 (MIS45_09070) - 1857585..1857830 (+) 246 WP_249450289.1 helix-turn-helix domain-containing protein -

Sequence

Protein


Download         Length: 346 a.a.        Molecular weight: 38123.85 Da        Isoelectric Point: 7.6075

>NTDB_id=656782 MIS45_RS09055 WP_249446020.1 1853753..1854793(+) (pilT) [Wielerella bovis strain CCUG 44465]
MQITDLLAFGVKNKASDLHLSAGLPPMIRVHGDVRRINLPEMSAEDVGNMISSIMNDLQRKNYQQNLETDFSFELPNVAR
FRVNAFNTNRGPAAVLRTIPSKVLTLEELKAPRIFQKIADTPRGLVLVTGPTGSGKSTTLAAMIDYVNDNHAGHILTIED
PIEFVHQSKKCLVNQRELHEHTHSFANALKSALREDPDIILVGEMRDPETIGLALTAAETGHLVFGTLHTTSAAKTVDRI
IDVFPAGEKEMVRSMLSESLRAVIAQTLLKTKDGKGRVAAHEIMVSTPAIRNLIRENKIAQMNSTIQTGQNFGMQTLDQC
LQDLVRRNIVSLADARAKAQNPDMIV

Nucleotide


Download         Length: 1041 bp        

>NTDB_id=656782 MIS45_RS09055 WP_249446020.1 1853753..1854793(+) (pilT) [Wielerella bovis strain CCUG 44465]
ATGCAAATTACCGACTTGCTCGCCTTTGGCGTGAAAAACAAAGCATCAGACTTGCACCTATCCGCAGGTTTGCCGCCAAT
GATTCGTGTACACGGCGATGTGCGCCGCATCAATCTGCCCGAAATGAGTGCGGAAGATGTGGGTAACATGATTAGTTCTA
TTATGAACGACTTGCAACGCAAAAACTATCAACAAAATTTGGAAACCGACTTTTCGTTTGAATTACCAAATGTGGCGCGT
TTCCGTGTCAATGCGTTTAACACCAATCGCGGACCCGCTGCTGTGTTGCGTACCATTCCCAGCAAAGTATTGACGCTGGA
AGAATTAAAAGCACCGCGTATTTTCCAAAAAATTGCCGATACACCGCGCGGTTTGGTTTTGGTAACAGGTCCAACAGGTT
CAGGTAAATCCACCACTTTGGCAGCGATGATTGACTATGTGAACGACAATCACGCAGGTCATATTTTAACGATTGAAGAC
CCAATTGAGTTTGTGCATCAATCCAAAAAATGCTTGGTAAACCAACGTGAATTGCACGAACACACACACAGTTTCGCCAA
CGCGCTGAAATCAGCCTTGCGTGAAGACCCAGATATTATTCTAGTGGGCGAGATGCGCGATCCTGAAACCATCGGTTTGG
CATTGACCGCTGCCGAAACAGGGCACTTGGTATTCGGTACTTTGCATACCACTTCCGCTGCCAAAACCGTTGACCGTATT
ATTGACGTGTTCCCTGCGGGCGAGAAAGAGATGGTTCGTTCCATGTTGTCCGAATCATTGCGCGCCGTAATCGCACAAAC
GCTGCTGAAAACCAAAGACGGTAAAGGTCGTGTTGCCGCGCATGAAATCATGGTTTCTACACCTGCGATTCGTAACTTGA
TTCGTGAAAACAAAATTGCCCAAATGAACTCCACCATTCAAACAGGTCAAAACTTTGGTATGCAGACATTAGACCAATGT
TTGCAAGATTTGGTGCGCCGCAACATCGTATCATTAGCTGATGCACGCGCCAAAGCACAAAACCCTGACATGATTGTGTA
A

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Neisseria gonorrhoeae MS11

80.466

99.133

0.798
  pilT Neisseria meningitidis 8013

80.175

99.133

0.795
  pilT Acinetobacter nosocomialis M2

73.469

99.133

0.728
  pilT Acinetobacter baumannii D1279779

73.469

99.133

0.728
  pilT Acinetobacter baumannii strain A118

73.469

99.133

0.728
  pilT Pseudomonas stutzeri DSM 10701

72.595

99.133

0.72
  pilT Acinetobacter baylyi ADP1

72.303

99.133

0.717
  pilT Pseudomonas aeruginosa PAK

72.303

99.133

0.717
  pilT Legionella pneumophila strain Lp02

69.565

99.711

0.694
  pilT Legionella pneumophila strain ERS1305867

69.565

99.711

0.694
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.353

98.266

0.662
  pilT Vibrio cholerae strain A1552

67.353

98.266

0.662
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.292

98.844

0.497
  pilU Pseudomonas stutzeri DSM 10701

43.284

96.821

0.419
  pilU Vibrio cholerae strain A1552

43.284

96.821

0.419
  pilU Acinetobacter baylyi ADP1

39.826

99.422

0.396