Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   MIS31_RS10170 Genome accession   NZ_CP092360
Coordinates   1989247..1990287 (+) Length   346 a.a.
NCBI ID   WP_249447735.1    Uniprot ID   -
Organism   Wielerella bovis strain 07LB258     
Function   pseudopilus biogenesis (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1984247..1995287
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MIS31_RS10140 (MIS31_10140) - 1984904..1985410 (-) 507 WP_249446628.1 GAF domain-containing protein -
  MIS31_RS10145 (MIS31_10145) - 1985675..1986496 (+) 822 WP_249446629.1 beta-1,6-N-acetylglucosaminyltransferase -
  MIS31_RS10150 (MIS31_10150) cyaY 1986583..1986909 (-) 327 WP_249443838.1 iron donor protein CyaY -
  MIS31_RS10155 (MIS31_10155) - 1986989..1987171 (+) 183 WP_249442299.1 lipoprotein -
  MIS31_RS10160 (MIS31_10160) - 1987198..1987572 (+) 375 WP_249446630.1 Imm8 family immunity protein -
  MIS31_RS10165 (MIS31_10165) lysA 1987588..1988808 (+) 1221 WP_249446631.1 diaminopimelate decarboxylase -
  MIS31_RS10170 (MIS31_10170) pilT 1989247..1990287 (+) 1041 WP_249447735.1 type IV pilus twitching motility protein PilT Machinery gene
  MIS31_RS10175 (MIS31_10175) - 1990311..1991636 (+) 1326 WP_249442302.1 PilT/PilU family type 4a pilus ATPase -
  MIS31_RS10180 (MIS31_10180) - 1991959..1994850 (-) 2892 WP_249446632.1 Rne/Rng family ribonuclease -

Sequence

Protein


Download         Length: 346 a.a.        Molecular weight: 38139.85 Da        Isoelectric Point: 7.6075

>NTDB_id=656759 MIS31_RS10170 WP_249447735.1 1989247..1990287(+) (pilT) [Wielerella bovis strain 07LB258]
MQITDLLAFGVKNKASDLHLSAGLPPMIRVHGDVRRINLPEMSAEDVGNMISSIMNDLQRKNYQQNLETDFSFELPNVAR
FRVNAFNTNRGPAAVLRTIPSKVLTLEELKAPRIFQKIADTPRGLVLVTGPTGSGKSTTLAAMIDYVNDNHAGHILTIED
PIEFVHQSKKCLVNQRELHEHTHSFANALKSALREDPDIILVGEMRDPETIGLALTAAETGHLVFGTLHTTSASKTVDRI
IDVFPAGEKEMVRSMLSESLRAVIAQTLLKTKDGKGRVAAHEIMVSTPAIRNLIRENKIAQMNSTIQTGQNFGMQTLDQC
LQDLVRRNIVSLADARAKAQNPDMIV

Nucleotide


Download         Length: 1041 bp        

>NTDB_id=656759 MIS31_RS10170 WP_249447735.1 1989247..1990287(+) (pilT) [Wielerella bovis strain 07LB258]
ATGCAAATTACCGACTTGCTCGCCTTTGGCGTGAAAAACAAAGCATCAGACTTGCACCTATCCGCAGGTTTGCCGCCAAT
GATTCGTGTACACGGCGATGTGCGCCGCATCAATCTGCCCGAAATGAGTGCGGAAGATGTGGGTAACATGATTAGTTCTA
TTATGAACGACTTGCAACGCAAAAACTATCAACAAAATTTGGAAACCGACTTTTCGTTTGAATTGCCAAATGTGGCGCGT
TTCCGTGTCAATGCGTTTAACACCAATCGCGGACCCGCTGCTGTGTTGCGTACCATTCCCAGCAAAGTATTGACGCTGGA
AGAATTAAAAGCACCGCGCATTTTCCAAAAAATTGCCGATACACCGCGCGGTTTGGTTTTGGTAACAGGTCCAACAGGTT
CAGGTAAATCCACCACTTTGGCAGCAATGATTGACTATGTGAACGACAATCACGCAGGTCATATTTTAACGATTGAAGAC
CCAATTGAGTTTGTGCATCAATCCAAAAAATGCTTGGTAAACCAACGCGAATTGCACGAACACACACACAGTTTCGCCAA
CGCGCTGAAATCAGCCTTGCGTGAAGACCCAGATATTATTCTAGTGGGCGAGATGCGCGACCCTGAAACCATCGGTTTGG
CATTGACCGCTGCCGAAACAGGGCACTTGGTATTCGGTACTTTGCATACCACATCCGCTTCCAAAACCGTTGACCGTATT
ATTGACGTGTTCCCTGCGGGCGAGAAAGAGATGGTTCGTTCCATGTTGTCCGAATCATTGCGCGCCGTAATCGCACAAAC
GCTGCTGAAAACCAAAGACGGTAAAGGCCGTGTTGCCGCGCATGAAATCATGGTTTCTACACCTGCGATTCGTAACTTGA
TTCGTGAAAACAAAATTGCCCAAATGAACTCCACCATTCAAACAGGTCAAAACTTTGGTATGCAGACATTAGACCAATGT
TTGCAAGATTTGGTGCGCCGCAACATCGTATCACTCGCCGATGCACGCGCCAAAGCACAAAACCCTGACATGATTGTGTA
A

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Neisseria gonorrhoeae MS11

80.175

99.133

0.795
  pilT Neisseria meningitidis 8013

79.883

99.133

0.792
  pilT Acinetobacter nosocomialis M2

73.178

99.133

0.725
  pilT Acinetobacter baumannii D1279779

73.178

99.133

0.725
  pilT Acinetobacter baumannii strain A118

73.178

99.133

0.725
  pilT Pseudomonas stutzeri DSM 10701

72.303

99.133

0.717
  pilT Pseudomonas aeruginosa PAK

72.012

99.133

0.714
  pilT Acinetobacter baylyi ADP1

72.012

99.133

0.714
  pilT Legionella pneumophila strain Lp02

69.565

99.711

0.694
  pilT Legionella pneumophila strain ERS1305867

69.565

99.711

0.694
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.059

98.266

0.659
  pilT Vibrio cholerae strain A1552

67.059

98.266

0.659
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.292

98.844

0.497
  pilU Pseudomonas stutzeri DSM 10701

43.284

96.821

0.419
  pilU Vibrio cholerae strain A1552

43.284

96.821

0.419
  pilU Acinetobacter baylyi ADP1

39.826

99.422

0.396