Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   MIS33_RS09565 Genome accession   NZ_CP092359
Coordinates   1915440..1916480 (+) Length   346 a.a.
NCBI ID   WP_249451605.1    Uniprot ID   -
Organism   Wielerella bovis strain 08LB461     
Function   pseudopilus biogenesis (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1910440..1921480
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MIS33_RS09535 (MIS33_09535) - 1911097..1911603 (-) 507 WP_249446628.1 GAF domain-containing protein -
  MIS33_RS09540 (MIS33_09540) - 1911868..1912689 (+) 822 WP_249446629.1 beta-1,6-N-acetylglucosaminyltransferase -
  MIS33_RS09545 (MIS33_09545) cyaY 1912776..1913102 (-) 327 WP_249443838.1 iron donor protein CyaY -
  MIS33_RS09550 (MIS33_09550) - 1913182..1913364 (+) 183 WP_249442299.1 lipoprotein -
  MIS33_RS09555 (MIS33_09555) - 1913391..1913765 (+) 375 WP_249451487.1 Imm8 family immunity protein -
  MIS33_RS09560 (MIS33_09560) lysA 1913781..1915001 (+) 1221 WP_249446631.1 diaminopimelate decarboxylase -
  MIS33_RS09565 (MIS33_09565) pilT 1915440..1916480 (+) 1041 WP_249451605.1 type IV pilus twitching motility protein PilT Machinery gene
  MIS33_RS09570 (MIS33_09570) - 1916504..1917829 (+) 1326 WP_249442302.1 PilT/PilU family type 4a pilus ATPase -
  MIS33_RS09575 (MIS33_09575) - 1918152..1921043 (-) 2892 WP_249446632.1 Rne/Rng family ribonuclease -

Sequence

Protein


Download         Length: 346 a.a.        Molecular weight: 38169.87 Da        Isoelectric Point: 7.6075

>NTDB_id=656733 MIS33_RS09565 WP_249451605.1 1915440..1916480(+) (pilT) [Wielerella bovis strain 08LB461]
MQITDLLAFGVKNKASDLHLSAGLPPMIRVHGDVRRINLPEMSAEDVGNMISSIMNDLQRKNYQQNLETDFSFELPNVAR
FRVNAFNTNRGPAAVLRTIPSKVLTLEELKAPRIFQKIADTPRGLVLVTGPTGSGKSTTLAAMIDYVNDNHAGHILTIED
PIEFVHQSKKCLVNQRELHEHTHSFANALKSALREDPDIILVGEMRDPETIGLALTAAETGHLVFGTLHTTSASKTVDRI
IDVFPAGEKEMVRSMLSESLRAVIAQTLLKTKDGKGRVAAHEIMVSTPAIRNLIRENKITQMNSTIQTGQNFGMQTLDQC
LQDLVRRNIVSLADARAKAQNPDMIV

Nucleotide


Download         Length: 1041 bp        

>NTDB_id=656733 MIS33_RS09565 WP_249451605.1 1915440..1916480(+) (pilT) [Wielerella bovis strain 08LB461]
ATGCAAATTACCGACTTGCTCGCCTTTGGCGTGAAAAACAAAGCATCAGACTTGCACCTATCCGCAGGTTTGCCGCCAAT
GATTCGTGTACACGGCGATGTGCGCCGCATCAATCTGCCCGAAATGAGTGCGGAAGATGTGGGTAACATGATTAGTTCTA
TTATGAACGACTTGCAACGCAAAAACTATCAACAAAATTTGGAAACCGACTTTTCGTTTGAATTGCCAAATGTGGCGCGT
TTCCGTGTCAATGCGTTTAACACCAATCGCGGACCCGCTGCTGTGTTGCGTACCATTCCCAGCAAAGTATTGACGCTGGA
AGAATTAAAAGCACCGCGCATTTTCCAAAAAATTGCCGATACACCGCGCGGTTTGGTTTTGGTAACAGGTCCAACAGGTT
CAGGTAAATCCACCACTTTGGCAGCAATGATTGACTATGTGAACGACAATCACGCAGGTCATATTTTAACGATTGAAGAC
CCAATTGAGTTTGTGCATCAATCCAAAAAATGCTTGGTAAACCAACGCGAATTGCACGAACACACACACAGTTTCGCCAA
CGCGCTGAAATCAGCCTTGCGTGAAGACCCAGATATTATTCTAGTGGGCGAGATGCGCGACCCTGAAACCATCGGTTTGG
CATTGACCGCTGCCGAAACAGGGCACTTGGTATTCGGTACTTTGCATACCACATCCGCTTCCAAAACCGTTGACCGTATT
ATTGACGTGTTCCCTGCGGGCGAGAAAGAGATGGTTCGTTCCATGTTGTCCGAATCATTGCGCGCCGTAATCGCACAAAC
GCTGCTGAAAACCAAAGACGGTAAAGGCCGTGTTGCCGCGCATGAAATCATGGTTTCTACACCTGCGATTCGTAACTTGA
TTCGTGAAAACAAAATTACCCAAATGAACTCCACCATTCAAACAGGTCAAAACTTTGGTATGCAGACATTAGACCAATGT
TTGCAAGATTTGGTGCGCCGCAACATCGTATCACTCGCCGATGCACGCGCCAAAGCACAAAACCCTGACATGATTGTGTA
A

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Neisseria gonorrhoeae MS11

80.466

99.133

0.798
  pilT Neisseria meningitidis 8013

80.175

99.133

0.795
  pilT Acinetobacter nosocomialis M2

72.886

99.133

0.723
  pilT Acinetobacter baumannii D1279779

72.886

99.133

0.723
  pilT Acinetobacter baumannii strain A118

72.886

99.133

0.723
  pilT Pseudomonas stutzeri DSM 10701

72.012

99.133

0.714
  pilT Pseudomonas aeruginosa PAK

71.72

99.133

0.711
  pilT Acinetobacter baylyi ADP1

71.72

99.133

0.711
  pilT Legionella pneumophila strain Lp02

69.275

99.711

0.691
  pilT Legionella pneumophila strain ERS1305867

69.275

99.711

0.691
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

66.765

98.266

0.656
  pilT Vibrio cholerae strain A1552

66.765

98.266

0.656
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.292

98.844

0.497
  pilU Pseudomonas stutzeri DSM 10701

43.284

96.821

0.419
  pilU Vibrio cholerae strain A1552

43.284

96.821

0.419
  pilU Acinetobacter baylyi ADP1

39.826

99.422

0.396