Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   MIS44_RS04470 Genome accession   NZ_CP092357
Coordinates   835909..836949 (-) Length   346 a.a.
NCBI ID   WP_249449580.1    Uniprot ID   -
Organism   Wielerella bovis strain 10LB456     
Function   pseudopilus biogenesis (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 830909..841949
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MIS44_RS04455 (MIS44_04455) - 830945..831100 (+) 156 WP_249445692.1 hypothetical protein -
  MIS44_RS04460 (MIS44_04460) - 831346..834237 (+) 2892 WP_249449313.1 Rne/Rng family ribonuclease -
  MIS44_RS04465 (MIS44_04465) - 834560..835885 (-) 1326 WP_249442302.1 PilT/PilU family type 4a pilus ATPase -
  MIS44_RS04470 (MIS44_04470) pilT 835909..836949 (-) 1041 WP_249449580.1 type IV pilus twitching motility protein PilT Machinery gene
  MIS44_RS04475 (MIS44_04475) lysA 837124..838344 (-) 1221 WP_249449314.1 diaminopimelate decarboxylase -
  MIS44_RS04480 (MIS44_04480) - 838360..838734 (-) 375 WP_249449315.1 Imm8 family immunity protein -
  MIS44_RS04485 (MIS44_04485) - 838761..838943 (-) 183 WP_249442299.1 lipoprotein -
  MIS44_RS04490 (MIS44_04490) cyaY 839023..839349 (+) 327 WP_249449581.1 iron donor protein CyaY -
  MIS44_RS04495 (MIS44_04495) - 839436..840257 (-) 822 WP_249449316.1 beta-1,6-N-acetylglucosaminyltransferase -
  MIS44_RS04500 (MIS44_04500) - 840522..841028 (+) 507 WP_249449317.1 GAF domain-containing protein -

Sequence

Protein


Download         Length: 346 a.a.        Molecular weight: 38138.86 Da        Isoelectric Point: 8.0273

>NTDB_id=656668 MIS44_RS04470 WP_249449580.1 835909..836949(-) (pilT) [Wielerella bovis strain 10LB456]
MQITDLLAFGVKNKASDLHLSAGLPPMIRVHGDVRRINLPEMSAEDVGNMISSIMNDLQRKNYQQNLETDFSFELPNVAR
FRVNAFNTNRGPAAVLRTIPSKVLTLEELKAPRIFQKIADTPRGLVLVTGPTGSGKSTTLAAMIDYVNDNHAGHILTIED
PIEFVHQSKKCLVNQRELHEHTHSFANALKSALREDPDIILVGEMRDPETIGLALTAAETGHLVFGTLHTTSASKTVNRI
IDVFPAGEKEMVRSMLSESLRAVIAQTLLKTKDGKGRVAAHEIMVSTPAIRNLIRENKIAQMNSTIQTGQNFGMQTLDQC
LQDLVRRNIVSLADARAKAQNPDMIV

Nucleotide


Download         Length: 1041 bp        

>NTDB_id=656668 MIS44_RS04470 WP_249449580.1 835909..836949(-) (pilT) [Wielerella bovis strain 10LB456]
ATGCAAATTACCGACTTGCTCGCCTTTGGCGTGAAAAACAAAGCATCAGACTTGCACCTATCCGCAGGTTTGCCGCCAAT
GATTCGTGTACACGGCGATGTGCGCCGCATCAATCTGCCCGAAATGAGTGCGGAAGATGTGGGTAACATGATTAGTTCTA
TTATGAACGACTTGCAACGCAAAAACTATCAACAAAATTTGGAAACCGACTTTTCGTTTGAATTGCCAAATGTGGCGCGT
TTCCGTGTCAATGCGTTTAACACCAATCGCGGACCCGCTGCTGTGTTGCGTACCATTCCCAGCAAAGTATTGACGCTGGA
AGAATTAAAAGCACCGCGTATTTTCCAAAAAATTGCCGATACACCGCGCGGTTTGGTTTTGGTAACAGGTCCAACAGGTT
CAGGTAAATCCACCACTTTGGCAGCGATGATTGACTATGTGAACGACAATCACGCAGGTCATATTTTAACGATTGAAGAC
CCAATTGAGTTTGTGCATCAATCCAAAAAATGCTTGGTAAACCAACGCGAATTGCACGAACACACACACAGTTTCGCCAA
CGCGCTGAAATCAGCCTTGCGTGAAGACCCAGATATTATTCTAGTGGGCGAGATGCGCGACCCTGAAACCATCGGTTTGG
CATTGACCGCTGCCGAAACAGGGCACTTGGTATTCGGTACTTTGCATACCACATCCGCTTCCAAAACCGTTAACCGTATT
ATTGACGTGTTCCCTGCGGGCGAGAAAGAGATGGTTCGTTCCATGTTGTCCGAATCATTACGCGCCGTAATCGCACAAAC
GCTGCTGAAAACCAAAGACGGTAAAGGTCGTGTTGCTGCACATGAAATCATGGTTTCTACACCTGCGATTCGTAACTTGA
TTCGTGAAAACAAAATTGCCCAAATGAACTCCACCATTCAAACAGGTCAAAACTTTGGTATGCAGACATTAGACCAATGT
TTGCAAGATTTGGTGCGCCGCAATATCGTATCATTAGCTGATGCACGCGCCAAAGCACAAAACCCTGACATGATTGTGTA
A

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Neisseria gonorrhoeae MS11

79.883

99.133

0.792
  pilT Neisseria meningitidis 8013

79.592

99.133

0.789
  pilT Acinetobacter nosocomialis M2

72.886

99.133

0.723
  pilT Acinetobacter baumannii D1279779

72.886

99.133

0.723
  pilT Acinetobacter baumannii strain A118

72.886

99.133

0.723
  pilT Pseudomonas stutzeri DSM 10701

72.012

99.133

0.714
  pilT Acinetobacter baylyi ADP1

71.72

99.133

0.711
  pilT Pseudomonas aeruginosa PAK

71.72

99.133

0.711
  pilT Legionella pneumophila strain Lp02

69.855

99.711

0.697
  pilT Legionella pneumophila strain ERS1305867

69.855

99.711

0.697
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

66.765

98.266

0.656
  pilT Vibrio cholerae strain A1552

66.765

98.266

0.656
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50

98.844

0.494
  pilU Vibrio cholerae strain A1552

43.284

96.821

0.419
  pilU Pseudomonas stutzeri DSM 10701

42.985

96.821

0.416
  pilU Acinetobacter baylyi ADP1

39.826

99.422

0.396