Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   LPY66_RS07310 Genome accession   NZ_CP092282
Coordinates   1542750..1543802 (+) Length   350 a.a.
NCBI ID   WP_337987422.1    Uniprot ID   -
Organism   Dehalobacter sp. DCM     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1537750..1548802
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LPY66_RS07290 (LPY66_07255) - 1537853..1538089 (+) 237 WP_337987418.1 hypothetical protein -
  LPY66_RS07295 (LPY66_07260) - 1538116..1538334 (+) 219 WP_337987419.1 hypothetical protein -
  LPY66_RS07300 (LPY66_07265) - 1538407..1540680 (+) 2274 WP_337987420.1 SpaA isopeptide-forming pilin-related protein -
  LPY66_RS07305 (LPY66_07270) - 1540992..1542740 (+) 1749 WP_337987421.1 GspE/PulE family protein -
  LPY66_RS07310 (LPY66_07275) pilT 1542750..1543802 (+) 1053 WP_337987422.1 type IV pilus twitching motility protein PilT Machinery gene
  LPY66_RS07315 (LPY66_07280) - 1543839..1545038 (+) 1200 WP_337987423.1 type II secretion system F family protein -
  LPY66_RS07320 - 1545148..1545576 (+) 429 WP_337987424.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  LPY66_RS07325 (LPY66_07290) - 1545813..1545974 (-) 162 WP_337987425.1 hypothetical protein -
  LPY66_RS07330 (LPY66_07295) pilM 1546202..1547200 (+) 999 WP_337987426.1 pilus assembly protein PilM -
  LPY66_RS07335 (LPY66_07300) - 1547237..1547779 (+) 543 WP_337987427.1 PilN domain-containing protein -

Sequence

Protein


Download         Length: 350 a.a.        Molecular weight: 39282.08 Da        Isoelectric Point: 7.0272

>NTDB_id=656457 LPY66_RS07310 WP_337987422.1 1542750..1543802(+) (pilT) [Dehalobacter sp. DCM]
MDIFELFRHTYDAKASDLHITVGIPPVIRANGSLRSLAYEALTPTDVMSLLQQIMKPVQLKELENKHELDFSFSHSGFGR
FRVNVYKQRGTFSAAFRSVMGRIPSIEELGLPPVINELTSKQRGLILVTGPTGSGKTTTLAYMIDKMNHERKSHIITLED
PIEYLYKHDQCIINQREVGSDVDSFSNGLRAALRQDPDVILVGEMRDLETISIAVTAAETGHLVLSTLHTVGAAKTIDRI
VDVFPPHQQQQIRIQLSTVLEGIVSQQLLPTADQKSRTVAVEVMLANNAIRNLIRENKTYQIQNVIQTGAKQGMQTMDGS
LLSLYRQGTITQEDVFKYCMETDIVRRYIN

Nucleotide


Download         Length: 1053 bp        

>NTDB_id=656457 LPY66_RS07310 WP_337987422.1 1542750..1543802(+) (pilT) [Dehalobacter sp. DCM]
ATGGATATCTTTGAATTATTCCGGCATACGTACGACGCCAAAGCATCAGACCTGCATATCACCGTCGGCATCCCGCCGGT
GATTAGGGCTAACGGCAGCCTTAGATCGCTGGCTTACGAAGCGTTAACACCCACTGATGTGATGAGCTTGCTCCAGCAGA
TCATGAAGCCAGTGCAGTTGAAAGAACTGGAAAATAAGCATGAATTGGATTTTTCCTTTTCCCACAGCGGATTTGGCCGT
TTCAGGGTTAATGTCTATAAACAGAGAGGAACATTCAGCGCGGCTTTCCGATCGGTCATGGGCAGGATCCCCTCCATCGA
GGAGCTGGGACTGCCGCCGGTCATCAATGAGTTAACGAGCAAACAGCGCGGACTGATTTTAGTGACAGGGCCGACGGGAA
GCGGCAAAACGACAACCTTGGCCTACATGATCGATAAAATGAATCACGAACGAAAATCGCACATCATTACCTTGGAAGAT
CCGATCGAATATCTGTATAAACATGATCAATGCATTATTAACCAGCGGGAAGTCGGGAGTGATGTCGACAGCTTCTCCAA
TGGACTGCGGGCGGCACTGCGCCAGGATCCGGATGTTATCTTAGTCGGCGAGATGCGGGATCTGGAAACGATTAGCATTG
CGGTTACGGCAGCGGAAACAGGGCATTTGGTATTGTCTACACTGCATACGGTCGGTGCTGCGAAAACAATCGACCGTATC
GTTGATGTCTTTCCGCCACATCAGCAGCAGCAAATCCGGATTCAGCTCTCTACAGTCTTGGAAGGAATCGTCTCCCAGCA
ATTATTGCCGACGGCGGATCAAAAGAGCCGTACTGTCGCTGTAGAAGTGATGCTGGCCAATAACGCGATCCGCAACTTGA
TCCGCGAGAATAAAACCTATCAGATTCAAAATGTCATTCAAACCGGTGCAAAACAGGGGATGCAAACTATGGACGGAAGC
CTTCTCAGCTTATACCGTCAGGGGACGATCACCCAGGAAGACGTGTTTAAATATTGCATGGAGACGGATATCGTCAGACG
CTATATTAATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(8-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50

100

0.5
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.851

95.714

0.477
  pilT Vibrio cholerae strain A1552

49.851

95.714

0.477
  pilT Legionella pneumophila strain Lp02

50.301

94.857

0.477
  pilT Legionella pneumophila strain ERS1305867

50.301

94.857

0.477
  pilT Acinetobacter baylyi ADP1

50.303

94.286

0.474
  pilT Acinetobacter baumannii strain A118

49.547

94.571

0.469
  pilT Acinetobacter nosocomialis M2

49.547

94.571

0.469
  pilT Acinetobacter baumannii D1279779

49.547

94.571

0.469
  pilT Neisseria meningitidis 8013

49.249

95.143

0.469
  pilT Neisseria gonorrhoeae MS11

47.522

98

0.466
  pilT Pseudomonas stutzeri DSM 10701

48.949

95.143

0.466
  pilT Pseudomonas aeruginosa PAK

48.802

95.429

0.466
  pilU Pseudomonas stutzeri DSM 10701

40.688

99.714

0.406
  pilU Acinetobacter baylyi ADP1

40.588

97.143

0.394
  pilU Vibrio cholerae strain A1552

41.796

92.286

0.386