Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   MF271_RS11295 Genome accession   NZ_CP092190
Coordinates   1722458..1723651 (+) Length   397 a.a.
NCBI ID   WP_239048887.1    Uniprot ID   -
Organism   Deinococcus sp. KNUC1210     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1717458..1728651
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MF271_RS11280 (MF271_11280) pgeF 1718204..1718983 (+) 780 WP_239048885.1 peptidoglycan editing factor PgeF -
  MF271_RS11285 (MF271_11285) - 1718980..1719618 (+) 639 WP_370657300.1 YqeG family HAD IIIA-type phosphatase -
  MF271_RS11290 (MF271_11290) pilB 1719676..1722342 (+) 2667 WP_239048886.1 type II/IV secretion system protein Machinery gene
  MF271_RS11295 (MF271_11295) pilT 1722458..1723651 (+) 1194 WP_239048887.1 type IV pilus twitching motility protein PilT Machinery gene
  MF271_RS11300 (MF271_11300) - 1723755..1723976 (+) 222 WP_239048888.1 hypothetical protein -
  MF271_RS11305 (MF271_11305) - 1724312..1725235 (+) 924 WP_239048889.1 GNAT family N-acetyltransferase -
  MF271_RS11310 (MF271_11310) - 1725219..1725878 (-) 660 WP_239048890.1 DsbA family protein -
  MF271_RS11315 (MF271_11315) - 1725933..1726594 (+) 662 Protein_1723 trimeric intracellular cation channel family protein -
  MF271_RS11320 (MF271_11320) - 1726613..1727221 (-) 609 WP_239048891.1 TMEM175 family protein -
  MF271_RS11325 (MF271_11325) - 1727231..1727725 (-) 495 WP_239048892.1 CAP domain-containing protein -
  MF271_RS11330 (MF271_11330) - 1727770..1728534 (-) 765 WP_239048893.1 metallophosphoesterase -

Sequence

Protein


Download         Length: 397 a.a.        Molecular weight: 43370.51 Da        Isoelectric Point: 7.2031

>NTDB_id=656298 MF271_RS11295 WP_239048887.1 1722458..1723651(+) (pilT) [Deinococcus sp. KNUC1210]
MQTAADITDILRVAVEKGASDVILTASLPPQFKVHGVYSSFDFTELTPTETRKLMYSMMNEKQQRNFEEKRELDFSFALG
DKARFRVNTFVQRGSVGGVMRLIPTNIKSVGDLGLPQSVVDVANAPRGLVLVTGPTGSGKSTTLAAMIDYINTNKKLHIV
TIEDPIEFMHQHKSSIINQREVGSDTHDFQNALRAVLRQAPDVILVGEMRDYETIKAAVTAAETGHLVMGTLHTNSAPES
IDRIVDVFPEEQQEQIRVQLANNLVAVMTQQLLPRIDGGRVLAYELLIANPAVRALIREGKTYQIMSVMQTGAREGMVTM
DAYLAGLYRRRLITHDIGMERAVDSKEFARLAADPSAGNALTAGLNTAANGMGSQPRPTATTVSTPSSTAGNPYGRR

Nucleotide


Download         Length: 1194 bp        

>NTDB_id=656298 MF271_RS11295 WP_239048887.1 1722458..1723651(+) (pilT) [Deinococcus sp. KNUC1210]
ATGCAAACAGCCGCCGACATCACCGATATTCTGCGCGTTGCCGTCGAGAAAGGCGCCAGCGACGTGATTCTGACCGCCTC
GCTGCCGCCCCAGTTCAAGGTACACGGCGTGTATTCGAGCTTCGACTTCACCGAACTGACCCCGACCGAGACGCGCAAGC
TGATGTACAGCATGATGAACGAGAAACAGCAGCGCAACTTCGAGGAAAAACGCGAGCTGGACTTCTCGTTTGCGCTCGGC
GACAAGGCCCGTTTCCGCGTCAATACCTTCGTGCAGCGCGGCAGCGTGGGCGGCGTGATGCGTCTGATCCCCACCAATAT
CAAGTCGGTGGGTGACCTCGGGCTGCCGCAGAGCGTGGTCGATGTCGCCAACGCGCCGCGTGGTCTGGTGCTGGTGACCG
GTCCCACCGGCTCGGGCAAATCGACCACGCTGGCCGCCATGATCGACTACATCAACACCAACAAGAAGCTGCACATCGTG
ACCATCGAAGACCCCATCGAGTTCATGCATCAGCACAAGAGCAGCATCATCAATCAGCGCGAGGTCGGGTCAGATACCCA
CGACTTCCAGAACGCGCTCAGAGCGGTGCTGCGTCAGGCCCCCGACGTGATTCTGGTGGGCGAAATGCGAGACTACGAGA
CCATCAAGGCCGCCGTGACCGCCGCCGAAACCGGACACCTGGTGATGGGAACGCTGCACACCAACAGCGCTCCGGAGAGC
ATCGACCGTATCGTAGACGTCTTCCCCGAGGAGCAGCAGGAACAGATCCGGGTACAGCTCGCCAACAACCTGGTCGCCGT
CATGACGCAGCAGCTCCTGCCCCGTATCGACGGCGGACGCGTGCTGGCCTACGAACTCCTGATCGCCAATCCGGCGGTTC
GCGCCCTGATCCGCGAGGGCAAGACCTACCAGATCATGAGCGTGATGCAGACCGGTGCACGTGAAGGCATGGTCACGATG
GACGCCTATCTGGCAGGCCTGTACCGCCGACGCCTCATCACGCACGACATCGGCATGGAACGTGCGGTGGACAGCAAGGA
ATTCGCACGTCTGGCCGCCGATCCGTCTGCGGGCAATGCACTTACCGCCGGACTCAACACCGCAGCCAACGGCATGGGCA
GTCAGCCACGTCCCACCGCCACCACGGTCAGTACGCCTTCGAGCACCGCCGGCAACCCCTACGGACGGCGATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-274)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

76.071

100

0.761
  pilT Neisseria meningitidis 8013

51.884

86.902

0.451
  pilT Acinetobacter baumannii D1279779

54.407

82.872

0.451
  pilT Acinetobacter baumannii strain A118

54.407

82.872

0.451
  pilT Acinetobacter nosocomialis M2

54.407

82.872

0.451
  pilT Neisseria gonorrhoeae MS11

51.163

86.65

0.443
  pilT Pseudomonas stutzeri DSM 10701

51.02

86.398

0.441
  pilT Pseudomonas aeruginosa PAK

51.02

86.398

0.441
  pilT Acinetobacter baylyi ADP1

53.049

82.62

0.438
  pilT Legionella pneumophila strain Lp02

48.98

86.398

0.423
  pilT Legionella pneumophila strain ERS1305867

48.98

86.398

0.423
  pilT Vibrio cholerae strain A1552

50.305

82.62

0.416
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.305

82.62

0.416
  pilU Pseudomonas stutzeri DSM 10701

46.356

86.398

0.401
  pilU Acinetobacter baylyi ADP1

42.241

87.657

0.37