Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   MF265_RS21350 Genome accession   NZ_CP092184
Coordinates   4425675..4426697 (-) Length   340 a.a.
NCBI ID   WP_239043868.1    Uniprot ID   -
Organism   Serratia marcescens strain YHYF1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4420675..4431697
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MF265_RS21320 (MF265_21320) - 4421488..4422000 (+) 513 WP_171454748.1 SprT family zinc-dependent metalloprotease -
  MF265_RS21325 (MF265_21325) endA 4422102..4422797 (+) 696 WP_085337148.1 deoxyribonuclease I -
  MF265_RS21330 (MF265_21330) rsmE 4422867..4423598 (+) 732 WP_239043865.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  MF265_RS21335 (MF265_21335) gshB 4423609..4424559 (+) 951 WP_239043866.1 glutathione synthase -
  MF265_RS21340 (MF265_21340) - 4424693..4425256 (+) 564 WP_239043867.1 YqgE/AlgH family protein -
  MF265_RS21345 (MF265_21345) ruvX 4425256..4425678 (+) 423 WP_033632095.1 Holliday junction resolvase RuvX -
  MF265_RS21350 (MF265_21350) pilT 4425675..4426697 (-) 1023 WP_239043868.1 type IV pilus twitching motility protein PilT Machinery gene
  MF265_RS21355 (MF265_21355) - 4426718..4427425 (+) 708 WP_015379048.1 YggS family pyridoxal phosphate-dependent enzyme -
  MF265_RS21360 (MF265_21360) proC 4427445..4428266 (+) 822 WP_004937440.1 pyrroline-5-carboxylate reductase -
  MF265_RS21365 (MF265_21365) - 4428298..4428852 (+) 555 WP_033632091.1 YggT family protein -
  MF265_RS21370 (MF265_21370) yggU 4428849..4429142 (+) 294 WP_016930091.1 DUF167 family protein YggU -
  MF265_RS21375 (MF265_21375) - 4429283..4429876 (+) 594 WP_171454752.1 XTP/dITP diphosphatase -
  MF265_RS21380 (MF265_21380) hemW 4429869..4431011 (+) 1143 WP_088381924.1 radical SAM family heme chaperone HemW -
  MF265_RS21385 (MF265_21385) - 4431049..4431483 (-) 435 WP_019456096.1 DUF29 domain-containing protein -

Sequence

Protein


Download         Length: 340 a.a.        Molecular weight: 36388.80 Da        Isoelectric Point: 8.2066

>NTDB_id=656212 MF265_RS21350 WP_239043868.1 4425675..4426697(-) (pilT) [Serratia marcescens strain YHYF1]
MDIDEFVALSVKHNASDLHLCAGHPPMLRIDGELQPLAAAPTLTAQGVQALCNGLLNAQQRESLRRLGQVDLALHRPSGE
RLRANVFQQSAGISLALRRIAGQSPSLAELAAPAIIPALLRCADGLILVTGATGSGKSTTLAAMIDEINRHQPRHVLTLE
DPIEFLHRSRRSLIQQREIGRDSHSFDAALRAALREDPDVILLGELRDIATIRLALTAAETGHLVLATLHTRSAPQAVER
LVDVFPAEEKPYVRAQLAGSLQAVIAQKLMRRPGGGRVAIFEVLTATAAVSNLIREGKTHQLASVLQTGAQSGMQTFKQG
LQQRIDAGVLGECAGEGTRG

Nucleotide


Download         Length: 1023 bp        

>NTDB_id=656212 MF265_RS21350 WP_239043868.1 4425675..4426697(-) (pilT) [Serratia marcescens strain YHYF1]
ATGGATATCGATGAATTCGTGGCCCTTAGTGTAAAGCATAATGCTTCCGATCTGCACCTTTGTGCCGGTCATCCGCCGAT
GTTGCGCATCGACGGTGAACTGCAGCCGCTGGCGGCGGCGCCAACGCTGACGGCGCAAGGCGTGCAGGCCCTTTGCAACG
GATTGCTGAATGCGCAGCAGCGCGAGAGCCTGCGGCGGCTGGGGCAGGTCGATCTGGCGTTGCACAGGCCGAGCGGGGAG
CGGCTGCGGGCCAACGTTTTTCAGCAAAGCGCGGGGATTTCTCTCGCGTTGCGGCGTATCGCCGGGCAATCGCCTTCGCT
TGCCGAGCTGGCGGCGCCGGCCATCATCCCGGCGCTGCTGCGGTGCGCCGACGGATTGATCCTGGTCACCGGCGCCACCG
GCAGCGGCAAGTCCACCACGCTGGCGGCGATGATCGACGAGATCAACCGGCACCAGCCGCGGCACGTTTTGACGCTGGAG
GATCCGATCGAATTCCTGCACCGCAGCCGGCGATCGCTGATCCAGCAGCGTGAGATCGGCCGTGACAGCCACAGCTTCGA
TGCGGCGTTGCGCGCCGCGCTGCGCGAAGATCCGGATGTGATCCTGCTGGGTGAGCTGCGCGATATTGCCACCATCCGGC
TGGCGCTCACCGCGGCGGAGACCGGGCATCTGGTGCTGGCGACGCTGCACACCCGCAGCGCGCCGCAGGCGGTGGAACGG
CTGGTGGACGTGTTTCCGGCGGAAGAGAAACCCTATGTGCGCGCCCAGCTGGCCGGCAGCCTGCAAGCGGTGATCGCGCA
AAAGCTGATGAGGCGCCCTGGCGGCGGACGCGTGGCGATCTTTGAAGTGCTGACGGCGACGGCGGCGGTCAGCAACCTGA
TCCGCGAAGGGAAAACCCATCAGTTGGCGAGCGTGTTGCAAACCGGCGCGCAGTCCGGCATGCAAACCTTTAAGCAAGGT
TTGCAGCAGCGGATCGACGCCGGCGTGTTGGGGGAGTGCGCGGGGGAGGGAACGAGGGGTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

54.242

97.059

0.526
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

54.242

97.059

0.526
  pilT Acinetobacter baylyi ADP1

51.057

97.353

0.497
  pilT Acinetobacter baumannii D1279779

50.606

97.059

0.491
  pilT Acinetobacter baumannii strain A118

50.606

97.059

0.491
  pilT Acinetobacter nosocomialis M2

50.606

97.059

0.491
  pilT Neisseria meningitidis 8013

50.453

97.353

0.491
  pilT Neisseria gonorrhoeae MS11

50.151

97.353

0.488
  pilT Legionella pneumophila strain ERS1305867

49.096

97.647

0.479
  pilT Legionella pneumophila strain Lp02

49.096

97.647

0.479
  pilT Pseudomonas aeruginosa PAK

49.091

97.059

0.476
  pilT Pseudomonas stutzeri DSM 10701

48.485

97.059

0.471
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.258

100

0.453
  pilU Vibrio cholerae strain A1552

40.123

95.294

0.382
  pilU Acinetobacter baylyi ADP1

39.628

95

0.376
  pilU Pseudomonas stutzeri DSM 10701

38.509

94.706

0.365