Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   OXB_RS15545 Genome accession   NZ_AP013294
Coordinates   3108281..3109318 (-) Length   345 a.a.
NCBI ID   WP_041075346.1    Uniprot ID   A0A0A8JLD1
Organism   Bacillus sp. OxB-1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3103281..3114318
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OXB_RS15520 (OXB_3114) - 3103948..3104637 (-) 690 WP_052484104.1 hypothetical protein -
  OXB_RS15525 (OXB_3115) - 3104630..3105322 (-) 693 WP_041075342.1 hypothetical protein -
  OXB_RS15530 (OXB_3116) pilM 3105325..3106263 (-) 939 WP_041075343.1 pilus assembly protein PilM -
  OXB_RS15535 (OXB_3117) - 3106528..3106944 (-) 417 WP_041075344.1 type II secretion system protein -
  OXB_RS15540 (OXB_3118) - 3107067..3108278 (-) 1212 WP_041075345.1 type II secretion system F family protein -
  OXB_RS15545 (OXB_3119) pilT 3108281..3109318 (-) 1038 WP_041075346.1 type IV pilus twitching motility protein PilT Machinery gene
  OXB_RS15550 (OXB_3120) - 3109330..3110988 (-) 1659 WP_041075347.1 ATPase, T2SS/T4P/T4SS family -
  OXB_RS15555 (OXB_3121) - 3111003..3112469 (-) 1467 WP_041075348.1 VanW family protein -
  OXB_RS15560 (OXB_3122) - 3112459..3112824 (-) 366 WP_144399727.1 hypothetical protein -
  OXB_RS18120 (OXB_3123) - 3112918..3114063 (-) 1146 WP_052484105.1 hypothetical protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38129.56 Da        Isoelectric Point: 6.8414

>NTDB_id=65604 OXB_RS15545 WP_041075346.1 3108281..3109318(-) (pilT) [Bacillus sp. OxB-1]
MKQVIDNLLTAAFKAKASDIHLTVGVPPIFRVHGDLKRYGENPVDENFTESVAQLTVPGKLYSQFKEHGQIDYSYEVAGA
ARFRVNAFQQRGNVSLAFRTIPTQIPTIEDLQMPPTLKNLAETRQGLILVTGPTGSGKSTTLAAMIRYINESMRRHIITL
EDPIEYVHQHGASIIDQREVGFDTDSFAAGLRAALRQDPDVILVGEMRDLETISTAITAAETGHLVFATLHTWSAASTID
RIIDVFPPGQQAQIRVQLAGVLTAVISQRLFSTADRQGRRAATEIMIKNPAIANLIRTEKVHQIPNVIQTNRGAGMHMMA
MSVKNFLDQGIITYENARPYIEGEE

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=65604 OXB_RS15545 WP_041075346.1 3108281..3109318(-) (pilT) [Bacillus sp. OxB-1]
ATGAAGCAAGTGATCGACAACTTATTGACAGCGGCGTTCAAGGCGAAGGCGTCCGATATTCATCTGACCGTCGGCGTGCC
ACCGATATTCCGGGTGCATGGTGATTTGAAACGGTACGGAGAAAATCCGGTAGATGAAAATTTCACAGAGTCCGTTGCCC
AATTGACCGTACCTGGAAAATTATATAGTCAGTTCAAGGAGCATGGCCAAATCGACTATTCGTATGAAGTGGCAGGCGCT
GCGCGGTTCCGGGTGAACGCATTCCAACAGCGGGGCAATGTCTCCCTTGCGTTCCGTACGATTCCTACGCAAATTCCGAC
CATCGAGGATCTGCAGATGCCGCCGACGTTAAAAAACTTGGCCGAAACACGCCAAGGGCTCATTTTGGTGACGGGTCCGA
CCGGGTCGGGGAAATCGACAACATTGGCTGCGATGATCCGCTATATCAATGAATCAATGCGACGGCATATCATCACGTTG
GAAGATCCGATCGAATATGTCCATCAACACGGAGCCTCCATCATCGATCAGAGGGAAGTCGGATTCGATACTGATTCCTT
CGCGGCCGGCTTGCGGGCTGCATTGCGGCAGGATCCCGATGTCATACTTGTCGGGGAGATGCGGGATTTAGAGACGATTT
CGACAGCTATTACCGCGGCAGAGACTGGTCACCTCGTCTTTGCGACACTGCATACATGGAGCGCGGCGTCGACAATCGAC
CGGATCATCGATGTATTTCCGCCAGGACAACAAGCGCAAATTCGCGTGCAATTGGCAGGCGTGTTGACAGCTGTCATATC
CCAGCGTTTATTCTCGACAGCGGATCGACAAGGTAGACGGGCGGCGACCGAGATCATGATCAAGAACCCGGCAATCGCAA
ACTTGATCCGTACGGAGAAGGTTCATCAGATTCCGAACGTCATTCAGACGAATCGAGGGGCAGGGATGCATATGATGGCG
ATGTCCGTCAAAAACTTCCTTGATCAGGGGATCATCACGTATGAAAACGCTAGGCCTTATATAGAGGGTGAAGAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0A8JLD1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Neisseria meningitidis 8013

51.009

100

0.513
  pilT Neisseria gonorrhoeae MS11

50.72

100

0.51
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.351

96.522

0.496
  pilT Pseudomonas aeruginosa PAK

50.599

96.812

0.49
  pilT Acinetobacter baylyi ADP1

49.267

98.841

0.487
  pilT Acinetobacter nosocomialis M2

50

96.812

0.484
  pilT Acinetobacter baumannii D1279779

50

96.812

0.484
  pilT Acinetobacter baumannii strain A118

50

96.812

0.484
  pilT Pseudomonas stutzeri DSM 10701

49.701

96.812

0.481
  pilT Vibrio cholerae strain A1552

48.817

97.971

0.478
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.817

97.971

0.478
  pilT Legionella pneumophila strain Lp02

48.649

96.522

0.47
  pilT Legionella pneumophila strain ERS1305867

48.649

96.522

0.47
  pilU Pseudomonas stutzeri DSM 10701

42.943

96.522

0.414
  pilU Acinetobacter baylyi ADP1

40.719

96.812

0.394
  pilU Vibrio cholerae strain A1552

41.139

91.594

0.377

Multiple sequence alignment