Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   HP572_RS15740 Genome accession   NZ_CP092070
Coordinates   3511993..3513009 (-) Length   338 a.a.
NCBI ID   WP_240583696.1    Uniprot ID   -
Organism   Pectobacterium sp. PL64     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3506993..3518009
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HP572_RS15725 (HP572_15725) nsrR 3507342..3507767 (-) 426 WP_010279087.1 nitric oxide-sensing transcriptional repressor NsrR -
  HP572_RS15730 (HP572_15730) - 3508093..3509664 (+) 1572 WP_240583694.1 methyl-accepting chemotaxis protein -
  HP572_RS15735 (HP572_15735) - 3510192..3512024 (+) 1833 WP_240583695.1 diguanylate cyclase -
  HP572_RS15740 (HP572_15740) pilT 3511993..3513009 (-) 1017 WP_240583696.1 type IV pilus twitching motility protein PilT Machinery gene
  HP572_RS15745 (HP572_15745) - 3513030..3513743 (+) 714 WP_240583697.1 YggS family pyridoxal phosphate-dependent enzyme -
  HP572_RS15750 (HP572_15750) proC 3513862..3514683 (+) 822 WP_043902046.1 pyrroline-5-carboxylate reductase -
  HP572_RS15755 (HP572_15755) - 3514733..3515287 (+) 555 WP_010279107.1 YggT family protein -
  HP572_RS15760 (HP572_15760) yggU 3515284..3515574 (+) 291 WP_010279110.1 DUF167 family protein YggU -
  HP572_RS15765 (HP572_15765) - 3515592..3516185 (+) 594 WP_240583698.1 XTP/dITP diphosphatase -
  HP572_RS15770 (HP572_15770) hemW 3516178..3517320 (+) 1143 WP_240583699.1 radical SAM family heme chaperone HemW -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 36697.93 Da        Isoelectric Point: 6.4240

>NTDB_id=655742 HP572_RS15740 WP_240583696.1 3511993..3513009(-) (pilT) [Pectobacterium sp. PL64]
MELDEWMARSVKHNASDLHLCSGHPPVLRVDGQLQPENTLPRLTPEQVAQWCDAWLEPAQREQLRQAGQVDGALMLPDGQ
RLRVNVFRQREGLSAALRIIPSAQPSLEALHAPPVLSTLLEKPNGLILITGATGSGKSTTLAAMLGALNDSSDRHVITLE
DPIEFIHASRRCLIQQREIGAHSASFALALRAALREDPDVILLGELRDTETIRLALTAAETGHLVLSTLHTRSASQAVDR
LIDVFPGEEKAYVRSQLATCLQAVVTQRLLPAAQGGRIALYEVLTATAAVSNLIREGKTHQLPGLIQTGAAAGMQTFEQS
YQQRCRDGLISHSCDLAV

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=655742 HP572_RS15740 WP_240583696.1 3511993..3513009(-) (pilT) [Pectobacterium sp. PL64]
ATGGAGTTGGATGAGTGGATGGCGCGTAGTGTAAAACATAATGCCTCGGATCTGCACCTTTGTAGCGGTCACCCGCCGGT
GTTGCGTGTGGACGGGCAGCTACAGCCTGAAAATACCTTACCACGTTTAACGCCGGAGCAGGTGGCACAATGGTGTGATG
CCTGGCTCGAACCGGCTCAACGTGAGCAACTGCGGCAGGCTGGTCAGGTGGACGGCGCGTTAATGCTGCCCGATGGACAA
CGCCTGCGGGTGAATGTGTTTCGCCAACGTGAAGGGCTGTCTGCGGCGCTGCGTATTATCCCGTCCGCTCAGCCTTCATT
GGAAGCGCTACATGCGCCACCCGTTCTATCGACGCTGCTGGAGAAGCCGAACGGACTGATCCTGATTACCGGGGCGACGG
GGAGCGGCAAATCCACGACGCTGGCGGCCATGCTTGGGGCGTTAAATGACAGCAGCGATCGCCATGTGATTACGCTGGAA
GATCCGATCGAGTTTATCCATGCCAGCCGACGCTGTCTGATTCAACAGCGGGAGATCGGTGCGCACAGTGCATCGTTTGC
ACTGGCGCTACGGGCCGCATTGCGGGAGGACCCCGATGTGATTCTGCTGGGGGAATTGCGCGATACGGAGACGATTCGGC
TGGCGCTGACGGCGGCGGAAACCGGCCATCTGGTGTTGTCGACGCTGCATACACGTAGTGCATCGCAGGCGGTCGATCGT
CTGATTGACGTCTTTCCCGGTGAAGAGAAAGCCTATGTTCGTAGCCAGTTAGCTACCTGTTTGCAGGCCGTCGTGACGCA
AAGGCTGTTGCCCGCTGCGCAAGGTGGTCGAATCGCGCTTTATGAAGTGCTGACGGCGACTGCGGCAGTCAGTAACCTGA
TTCGGGAAGGGAAAACGCACCAGCTTCCCGGCCTGATTCAAACCGGAGCCGCTGCGGGAATGCAAACGTTCGAGCAAAGC
TACCAGCAGCGCTGTCGGGATGGGCTTATTTCACACAGTTGCGACCTTGCCGTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

51.515

97.633

0.503
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

51.515

97.633

0.503
  pilT Neisseria meningitidis 8013

50.904

98.225

0.5
  pilT Neisseria gonorrhoeae MS11

50.602

98.225

0.497
  pilT Pseudomonas stutzeri DSM 10701

49.547

97.929

0.485
  pilT Acinetobacter baylyi ADP1

49.245

97.929

0.482
  pilT Legionella pneumophila strain Lp02

49.245

97.929

0.482
  pilT Legionella pneumophila strain ERS1305867

49.245

97.929

0.482
  pilT Pseudomonas aeruginosa PAK

49.245

97.929

0.482
  pilT Acinetobacter nosocomialis M2

48.943

97.929

0.479
  pilT Acinetobacter baumannii D1279779

48.943

97.929

0.479
  pilT Acinetobacter baumannii strain A118

48.943

97.929

0.479
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.136

99.704

0.42
  pilU Pseudomonas stutzeri DSM 10701

40.541

98.521

0.399
  pilU Vibrio cholerae strain A1552

39.359

100

0.399
  pilU Acinetobacter baylyi ADP1

38.509

95.266

0.367