Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   MA641_RS15685 Genome accession   NZ_CP091997
Coordinates   3219066..3220046 (-) Length   326 a.a.
NCBI ID   WP_023223875.1    Uniprot ID   A0A3T9Y8K5
Organism   Salmonella enterica subsp. enterica serovar Kentucky strain N20-2289     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3214066..3225046
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MA641_RS15655 (MA641_15650) endA 3214524..3215231 (+) 708 WP_000286124.1 deoxyribonuclease I -
  MA641_RS15660 (MA641_15655) rsmE 3215308..3216039 (+) 732 WP_001222488.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  MA641_RS15665 (MA641_15660) gshB 3216059..3217006 (+) 948 WP_000593248.1 glutathione synthase -
  MA641_RS15670 (MA641_15665) - 3217222..3217785 (+) 564 WP_001053171.1 YqgE/AlgH family protein -
  MA641_RS15675 (MA641_15670) ruvX 3217785..3218201 (+) 417 WP_001285491.1 Holliday junction resolvase RuvX -
  MA641_RS15680 (MA641_15675) - 3218248..3218934 (-) 687 WP_023223876.1 IclR family transcriptional regulator -
  MA641_RS15685 (MA641_15680) pilT 3219066..3220046 (-) 981 WP_023223875.1 type IV pilus twitching motility protein PilT Machinery gene
  MA641_RS15690 (MA641_15685) - 3220064..3220768 (+) 705 WP_000997812.1 YggS family pyridoxal phosphate-dependent enzyme -
  MA641_RS15695 (MA641_15690) - 3220787..3221353 (+) 567 WP_001094848.1 YggT family protein -
  MA641_RS15700 (MA641_15695) yggU 3221350..3221640 (+) 291 WP_001277203.1 DUF167 family protein YggU -
  MA641_RS15705 (MA641_15700) - 3221648..3222241 (+) 594 WP_001174769.1 XTP/dITP diphosphatase -
  MA641_RS15710 (MA641_15705) hemW 3222234..3223370 (+) 1137 WP_023223874.1 radical SAM family heme chaperone HemW -
  MA641_RS15715 (MA641_15710) - 3223462..3224469 (-) 1008 WP_023223873.1 DUF1202 domain-containing protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 36153.44 Da        Isoelectric Point: 7.8166

>NTDB_id=654928 MA641_RS15685 WP_023223875.1 3219066..3220046(-) (pilT) [Salmonella enterica subsp. enterica serovar Kentucky strain N20-2289]
MNMEEIVTLSVKHNVSDLHLCNAWPARWRKQGRMEIAPFTAPDVDRLLLDWLNDAQQYQWRTHGQLDFAVSLSGTRRLRA
SAFTHQQGTSLALRLLPERCPDLAEIQMPPIVPALLASENGLILVTGATGCGKSTTLAAMVGHLNQHADKHILTLEDPIE
YRYTSKRCLIQQREIGQHCATFAAGLRAALRQDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKEPVRSQLAGSLRAVLSQKLEVDRQDGRVALFELLINTPATGNLIREGKLHQLAHVIQTGQQQGMMTFAQSAQWR
QAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=654928 MA641_RS15685 WP_023223875.1 3219066..3220046(-) (pilT) [Salmonella enterica subsp. enterica serovar Kentucky strain N20-2289]
ATGAATATGGAAGAAATTGTGACGCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGTAACGCCTGGCCTGCGCG
ATGGCGCAAACAAGGACGCATGGAGATCGCGCCTTTTACCGCGCCAGATGTCGACAGGCTTCTGCTTGATTGGCTTAATG
ATGCGCAACAATATCAGTGGCGAACGCACGGCCAGCTTGATTTCGCCGTCTCGCTGTCTGGCACGCGGCGCCTACGCGCC
AGTGCGTTCACACATCAACAGGGAACGTCGCTGGCGCTACGGCTATTGCCCGAGCGTTGTCCTGATCTGGCGGAAATCCA
GATGCCGCCGATAGTACCCGCACTGCTTGCCAGCGAGAACGGACTGATTCTGGTCACTGGCGCTACTGGCTGCGGGAAGT
CCACCACGCTGGCGGCGATGGTGGGACATCTTAATCAACATGCCGATAAACACATCCTGACGCTGGAAGATCCTATTGAG
TACCGTTACACCAGCAAGCGTTGCCTGATACAGCAGCGGGAAATCGGCCAGCATTGCGCCACTTTTGCCGCCGGGCTGCG
CGCAGCGTTACGTCAAGATCCCGATGTCATTTTACTGGGAGAGCTGCGTGACAGCGAGACCATTCGCCTGGCGCTGACGG
CGGCGGAAACGGGGCATCTGGTACTGGCAACCCTGCATACTCGCGGTGCGGCGCAGGCGGTGGAAAGATTAGTGGATAGT
TTTCCGGCGCAGGAAAAAGAACCGGTACGCAGTCAACTGGCGGGTAGTCTGCGCGCCGTACTATCACAAAAACTGGAGGT
CGACAGACAGGATGGGCGCGTGGCGTTGTTTGAATTACTGATTAATACGCCAGCCACAGGGAATTTGATCCGTGAAGGCA
AACTCCATCAGTTGGCCCACGTGATACAAACCGGGCAGCAGCAGGGGATGATGACATTCGCCCAGAGCGCGCAATGGCGT
CAGGCGCAGGGACGACTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(75-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3T9Y8K5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.318

100

0.485
  pilT Vibrio cholerae strain A1552

48.318

100

0.485
  pilT Neisseria meningitidis 8013

46.951

100

0.472
  pilT Neisseria gonorrhoeae MS11

46.646

100

0.469
  pilT Pseudomonas aeruginosa PAK

44.954

100

0.451
  pilT Acinetobacter baylyi ADP1

44.648

100

0.448
  pilT Acinetobacter baumannii D1279779

44.648

100

0.448
  pilT Acinetobacter baumannii strain A118

44.648

100

0.448
  pilT Pseudomonas stutzeri DSM 10701

44.648

100

0.448
  pilT Acinetobacter nosocomialis M2

44.343

100

0.445
  pilT Legionella pneumophila strain ERS1305867

44.34

97.546

0.433
  pilT Legionella pneumophila strain Lp02

44.34

97.546

0.433
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.453

97.546

0.414
  pilU Vibrio cholerae strain A1552

38.871

97.853

0.38
  pilU Pseudomonas stutzeri DSM 10701

36.637

100

0.374
  pilU Acinetobacter baylyi ADP1

36.42

99.387

0.362