Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   AXH83_RS05560 Genome accession   NZ_AP013035
Coordinates   1109144..1110247 (-) Length   367 a.a.
NCBI ID   WP_068549858.1    Uniprot ID   -
Organism   Thermosulfidibacter takaii ABI70S6     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1104144..1115247
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AXH83_RS05540 (TST_1067) dapF 1104770..1105564 (-) 795 WP_083498610.1 diaminopimelate epimerase -
  AXH83_RS05545 (TST_1068) lysA 1105566..1106822 (-) 1257 WP_068549855.1 diaminopimelate decarboxylase -
  AXH83_RS05550 (TST_1069) - 1106839..1107789 (-) 951 WP_068549856.1 hypothetical protein -
  AXH83_RS05555 (TST_1070) argH 1107762..1109144 (-) 1383 WP_068549857.1 argininosuccinate lyase -
  AXH83_RS05560 (TST_1071) pilT 1109144..1110247 (-) 1104 WP_068549858.1 type IV pilus twitching motility protein PilT Machinery gene
  AXH83_RS05565 (TST_1072) pilB 1110264..1111985 (-) 1722 WP_068549859.1 type IV-A pilus assembly ATPase PilB -
  AXH83_RS05575 (TST_1073) porA 1112188..1113303 (+) 1116 WP_068549860.1 hypothetical protein -
  AXH83_RS05580 (TST_1074) - 1113294..1114088 (+) 795 WP_068549861.1 thiamine pyrophosphate-dependent enzyme -

Sequence

Protein


Download         Length: 367 a.a.        Molecular weight: 41111.42 Da        Isoelectric Point: 8.0452

>NTDB_id=65431 AXH83_RS05560 WP_068549858.1 1109144..1110247(-) (pilT) [Thermosulfidibacter takaii ABI70S6]
MSESTYPATMEQLLRIAVEQNATDLHISTGNPPRIRVHGRLIKIEHLPPLTPADTQRLCYSVMNEHQRKIFEQNNEVDFS
FGIARLSRFRANVFMQRGSVAGAFRRIPFEIIPLENLGLPPVVKGFVTKPKGLILVTGPTGSGKSTTLAAMIDRINSTME
KHIITIEDPIEYLHQHKKCLVNQREVGADTQSFKDALRYVLRQDPDVVLIGEMRDLESIAAALTVAETGHLTFATLHTNS
AIETINRIIDVFPPHQQSQVRAQLSHVLEGIITQKLLPRSDGRGLVLAAEVLVPTPAVRNLIREDKIHQVYTVMQAGQSH
HGMQTMNQALASLYERGLITYEQAVTSSYNPDELIPMLDRIKMRLQA

Nucleotide


Download         Length: 1104 bp        

>NTDB_id=65431 AXH83_RS05560 WP_068549858.1 1109144..1110247(-) (pilT) [Thermosulfidibacter takaii ABI70S6]
ATGAGTGAGAGTACTTATCCAGCTACTATGGAGCAGTTGTTAAGGATAGCAGTTGAGCAAAATGCCACAGACCTGCACAT
AAGCACAGGAAATCCTCCTCGTATAAGGGTCCACGGAAGATTGATTAAAATAGAGCATCTACCTCCTCTGACTCCGGCGG
ATACCCAGAGGCTTTGTTACAGTGTTATGAACGAGCACCAGAGAAAGATTTTTGAGCAGAATAATGAGGTGGATTTTTCC
TTTGGCATTGCTAGGCTCTCAAGGTTCAGGGCAAATGTCTTCATGCAAAGGGGCTCTGTGGCCGGTGCTTTCAGGCGTAT
TCCTTTTGAGATTATTCCATTAGAGAACCTGGGCTTGCCTCCGGTCGTTAAGGGTTTTGTAACAAAGCCTAAAGGTCTTA
TCTTGGTTACAGGTCCTACAGGTTCCGGTAAGTCAACAACTTTGGCGGCTATGATAGATAGGATTAACTCAACTATGGAG
AAGCACATTATTACCATTGAGGATCCCATAGAGTATTTGCATCAACATAAAAAATGTCTTGTGAATCAAAGAGAAGTCGG
CGCGGATACACAGTCTTTTAAGGACGCTCTTAGATATGTATTAAGACAAGATCCCGACGTTGTACTAATTGGTGAAATGA
GAGATCTTGAAAGTATAGCCGCGGCTTTGACAGTTGCTGAAACGGGTCACTTGACCTTTGCTACATTGCATACAAACTCT
GCTATTGAAACCATTAACAGAATCATAGATGTTTTCCCTCCACATCAGCAGAGTCAGGTTAGAGCCCAGCTTTCACATGT
TTTGGAGGGCATCATAACTCAAAAGCTCTTACCCAGATCTGACGGGAGAGGTTTGGTTCTTGCTGCTGAGGTGTTGGTTC
CCACACCAGCTGTGAGGAACCTGATAAGGGAAGATAAGATTCACCAGGTTTACACTGTGATGCAGGCAGGACAGTCCCAC
CATGGTATGCAGACCATGAACCAAGCCTTGGCAAGCCTTTACGAAAGAGGCTTGATCACCTATGAACAAGCGGTCACAAG
CTCTTATAACCCAGACGAGCTTATACCCATGTTGGATAGAATAAAAATGAGGTTGCAGGCATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(15-277)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Legionella pneumophila strain Lp02

55.162

92.371

0.51
  pilT Legionella pneumophila strain ERS1305867

55.162

92.371

0.51
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.216

93.188

0.496
  pilT Neisseria meningitidis 8013

51.754

93.188

0.482
  pilT Pseudomonas aeruginosa PAK

51.312

93.46

0.48
  pilT Neisseria gonorrhoeae MS11

51.462

93.188

0.48
  pilT Pseudomonas stutzeri DSM 10701

50.729

93.46

0.474
  pilT Acinetobacter baumannii D1279779

50.581

93.733

0.474
  pilT Acinetobacter baumannii strain A118

50.581

93.733

0.474
  pilT Acinetobacter nosocomialis M2

50.581

93.733

0.474
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

51.807

90.463

0.469
  pilT Vibrio cholerae strain A1552

51.807

90.463

0.469
  pilT Acinetobacter baylyi ADP1

49.854

93.46

0.466
  pilU Pseudomonas stutzeri DSM 10701

44.126

95.095

0.42
  pilU Vibrio cholerae strain A1552

40.115

95.095

0.381
  pilU Acinetobacter baylyi ADP1

39.942

93.46

0.373

Multiple sequence alignment